5-Ethynyl-dUTP(5-EdUTP) - ≥95%, 100 mM in water , CAS No.111289-87-3(free acid)

CAS: 111289-87-3(free acid) Cat. No.: E275713 Molecular Weight: 492.16(free acid)

GRADE & PURITY ≥95%, 100 mM in water

Synonyms

5-Ethynyl-2'-deoxyuridine5'-triphosphatesodiumsalt

Storage

Store at -20°C,Desiccated

Shipped In

Ice chest + Ice pads

Overview

Product Description

Incorporated into DNA by PCR to produce ethynyl-functionalized DNA.

Product Application

ncorporation into DNA by PCR with family B polymerases (Pwo, Deep Vent exo- or KOD XL)

Incorporation into DNA by Primer Extension with family A polymerase Taq and family B polymerases (Pwo, Deep Vent exo- or KOD XL)

The resulting ethynyl-functionalized DNA can subsequently be processed via Cu(I)-catalyzed click chemistry that offers the choice

to introduce a Biotin group (via Azides of Biotin) for subsequent purification tasks

to introduce fluorescent group (via Azides of fluorescent dyes) for subsequent microscopic imaging

to crosslink the DNA to Azide-functionalized biomolecules e.g.proteins 

Specifications

Synonyms

5-Ethynyl-2'-deoxyuridine5'-triphosphatesodiumsalt

Specifications & Purity

≥95%, 100 mM in water

Biochemical and Physiological Mechanisms

Incorporated into DNA by Primer Extension with family A polymerase Taq and family B polymerases.

Storage

Store at -20°C, Desiccated

Shipped In

Ice chest + Ice pads

This product requires cold chain shipping. Ground and other economy services are not available.

Note

Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 6 months. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour. Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.

Purity

≥95%

Names and Identifiers

• Molecular Weight: 492.16(free acid)

Chemical and Physical Properties

• Solubility: Supplied in water (100 mM)