5MPN - ≥98% , CAS No.47208-82-2

CAS: 47208-82-2 Cat. No.: M649540 Formula: C₁₅H₁₉N₃O₄ Peso molecolare: 305.33 Numero EC: 664-935-1 PubChem CID: 4060327

QUALITÀ & PUREZZA ≥98%

Storage

Store at -20°C

Shipped In

Ice chest + Ice pads

Panoramica

5MPN is a first-in-class, potent, orally active and selective 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 4 ( PFKFB4 ) inhibitor. 5MPN appears to be a competitive inhibitor of the F6P binding site ( K i =8.6 μM). 5MPN does not inhibit PFK-1 or PFKFB3. 5MPN targets the sugar metabolism of tumors and suppresses proliferation of multiple human cancer cell lines

In Vitro

5MPN (0~30 μM; 24 hours; H460 cells) inhibits the expression of PFKFB4. 5MPN (0~50 μM; 0~72 hours; H460 NSCLC cells) first reduces the intracellular concentration of F2,6BP, glycolysis and ATP, which in turn results in a reduction in cell proliferation. 5MPN (0 and 10 μM; 6, 12 and 24 hours; H460 cells) induces cells apoptosis. 5MPN (0 and 10 μM; 6, 12 and 24 hours; H460 cells) arrests cell cycle progression. 5MPN (0.1, 1 or 10 µM) significantly inhibits PFKFB4 activity. 5MPN (H460 cells) leads to a dose-dependent decrease in the intracellular F2,6BP concentration. 5MPN (0~30 μM; over 48 hours; H460, H1299, H441, H522 and A549 cells) makes a dose-dependent reduction in cells growth. 5MPN (0~30 μM; 24 hours; H460 cells) inhibits PFKFB4 expression causing the observed reduction in H460 cell proliferation. 5MPN causes a G1 arrest in LLC cells in vitro similar to H460 cells. MCE has not independently confirmed the accuracy of these methods. They are for reference only. Western Blot AnalysisCell Line: H460 cells Concentration: 0~30 μΜ Incubation Time: 24 hours Result: Inhibited the expression of PFKFB4 . Cell Proliferation AssayCell Line: H460 NSCLC cells Concentration: 0~50 μM Incubation Time: 0~72 hours Result: Resulted in a reduction in cell proliferation. Apoptosis AnalysisCell Line: H460 cells Concentration: 0 and 10 μΜ Incubation Time: 6, 12 and 24 hours Result: Induced cells apoptosis. Cell Cycle AnalysisCell Line: H460 cells Concentration: 0 and 10 μΜ Incubation Time: 6, 12 and 24 hours Result: Arrested cell cycle progression.

In Vivo

5MPN (120 mg/kg; p.o.) suppresses the growth of Lewis lung carcinomas (LLC) grown in syngeneic mice and H460 human lung adenocarcinoma xenografts grown in athymic mice without affecting body weight . 5MPN causes a reduction in Ki67-positive cells in the LLC xenografts suggesting that 5MPN may be reducing cell cycle progression in vivo . MCE has not independently confirmed the accuracy of these methods. They are for reference only. Animal Model: C57BL/6 mice Dosage: 120 mg/kg Administration: P.o. Result: Suppressed the growth of Lewis lung carcinomas (LLC) grown in syngeneic mice and H460 human lung adenocarcinoma xenografts grown in athymic mice without affecting body weight.

Form:Solid

IC50& Target:KI: 8.6 μM (PFKFB4)

Specifiche

Specifiche e purezza

≥98%

Meccanismi biochimici e fisiologici

5MPN is a first-in-class, potent, orally active and selective 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 4 ( PFKFB4 ) inhibitor. 5MPN appears to be a competitive inhibitor of the F6P binding site ( K i =8.6 μM). 5MPN does not inhibit PFK-1 or PF

Condizioni di conservazione di stoccaggio

Store at -20°C

Spedito in

Ice chest + Ice pads

Questo prodotto richiede spedizione a catena fredda. I servizi di terra e altri servizi economici non sono disponibili.

Purezza

≥98%

Nomi e identificatori

Sorrisi canonici

COC1=CC=CC2=C(C=CN=C21)NCCCCCO[N+](=O)[O-]

Isomeri SMILES

COC1=CC=CC2=C(C=CN=C21)NCCCCCO[N+](=O)[O-]

PubChem CID

4060327

Peso molecolare

305.33

Proprietà chimiche e fisiche

• Solubilità: DMSO : 100 mg/mL (327.51 mM; Need ultrasonic)
• Peso molecolare: 305.330 g/mol
• XLogP3: 3.500
• Hydrogen Bond Donor Count: 1
• Hydrogen Bond Acceptor Count: 6
• Rotatable Bond Count: 8
• Exact Mass: 305.138 Da
• Monoisotopic Mass: 305.138 Da
• Topological Polar Surface Area: 89.200 Ų
• Heavy Atom Count: 22
• Formal Charge: 0
• Complexity: 337.000
• Isotope Atom Count: 0
• Defined Atom Stereocenter Count: 0
• Undefined Atom Stereocenter Count: 0
• Defined Bond Stereocenter Count: 0
• Undefined Bond Stereocenter Count: 0
• The total count of all stereochemical bonds: 0
• Covalently-Bonded Unit Count: 1

Domande frequenti

What is the purity of this product?

This product is supplied at ≥98% purity (chemical assay). Lot-specific values are stated on the Certificate of Analysis.

How should this product be stored?

Store at ?20 °C. Freezer storage is required to maintain the specified shelf life.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What are the CAS number, molecular formula and molecular weight?

The CAS Number is 47208-82-2, the molecular formula is C15H19N3O4, and the molecular weight is 305.33 g/mol.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.