6× His Peptide

Cat. No.: H743510

QUALITÀ & PUREZZA 5.0 mg/mL

Panoramica

The 6X His Peptide is commonly used for the competitive elution of His tag fusion proteins bound to His antibodies. It can be used to elute His tag fusion proteins from Anti-His Magnetic Beads and conventional Anti-His antibodies during immunoprecipitation.This product competitively elutes the 6X His fusion protein. The eluted protein sample does not contain the light and heavy chains of Anti-His antibody, thus effectively eliminating the interference of anibodies with downstream Western blot analysis.The amino acid sequence of 6X His Peptide is His-His-His-His-His-His (HHHHHH).The main parameters of 6X His Peptide are as follows

Precautions：

The solution of this product should be aliquoted and stored at -20℃ or lower temperatures to avoid repeated freeze-thaw cycles.This product is for R&D only. Not for drug, household, or other uses.For your safety and health, please wear a lab coat and disposable gloves during the operation.

Instructions for Use：

1. For 25mg provided in powder form, centrifuge at 8,000-12,000×g for 10-30 seconds before opening to collect the powder at the bottom of the tube. Dissolve it in TBS by gently pipetting or shaking. Do not vortex vigorously to avoid denaturation and inactivation of the peptide.

2. P9811-1mg and P9811-5mg can be directly used for preparing elution buffer.

3. Competitive elution with the 6× His Peptide: This elution method is non-denaturing, with high elution efficiency, and the eluted sample does not contain the light and heavy chains of His antibodies.a. Preparation of 6× His tag elution buffer: Based on the required amount of elution buffer, dilute an appropriate amount of 6× His Peptide stock solution with TBS to a final concentration of 150μg/ml.b. Add 100μl of 6×  His tag elution buffer (150μg/ml) to each immunoprecipitated sample, and incubate on ice or at 4℃ for 30 minutes to 2 hours with shaking. In order to improve the elution efficiency, the incubation time can be extended or the elution can be repeated. The volume of the 6× His tag elution buffer is generally 5 times that of the bead or gel suspension.c. Centrifuge at 6000×g for 30 seconds at 4℃, and carefully transfer the supernatant to a new tube. The supernatant contains the 6× His fusion protein and its protein complexes. Be careful not to touch the resin when taking the supernatant.

4. Store the eluted 6× His fusion protein at 4℃ for immediate use, or at -20℃ for long-term storage.

Specifiche

Product Name

6× His Peptide

Specifiche e purezza

5.0 mg/mL

Tipo di molecola

Peptidi

Product Line

Prodotti biologici

Stoccaggio e spedizione

Concentrazione

5.0 mg/mL

Condizioni di conservazione di stoccaggio

Store at -20°C

Spedito in

Ice chest + Ice pads

Stabilità e conservazione

Stored at 4℃ for up to 1 year.