GRADE & PURITYBioReagent?BioReagent grade — tested suitable for life-science and molecular-biology use. Use for cell culture, assays, and biochemical work needing biological compatibility.
BioReagent BioReagent for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Protected from light,Store at -20°C Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Panoramica
Aconitase (ACO) is an enzyme in the tricarboxylic acid cycle that catalyzes the conversion of citrate to isocitrate. Citrate itself is not easily oxidized; under the action of aconitase, through dehydration and hydration reactions, the hydroxyl group is transferred from the β-carbon atom to the α-carbon atom, generating isocitrate which is readily dehydrogenated and oxidized, preparing for further oxidative decarboxylation reactions. This product provides a simple, convenient, and rapid method for assaying ACO activity, suitable for detecting samples such as animal tissues, plant tissues, cells, and serum (plasma). Its principle is that ACO catalyzes the conversion of citrate to isocitrate. Isocitrate is then oxidatively decarboxylated, reducing NAD⁺ to generate NADH, resulting in an increase in absorbance at 340 nm.
Specifications
Specifiche e purezza
BioReagent
Grado
BioReagent
Applicazione
Enzyme activity assay
Tipo di campione
Plasma, Siero, Tessuti animali, Tessuti vegetali
Metodo di rilevamento
Colorimetric Method
Detection instrument
Microplate reader,UV Spectrophotometer
Detection wavelength
340nm
Stoccaggio e spedizione
Condizioni di conservazione di stoccaggio
Protected from light,Store at -20°C
Spedito in
Ice chest + Ice pads
Stabilità e conservazione
Store at -20℃ for 6 months. Store in the dark.
Documentazione
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
Certificati (CoA, COO, BSE/TSE e tabella di analisi)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:
Calcolatori di soluzioni
Molarity Calculator
Determine the necessary mass, volume, or concentration for preparing a solution.
Dilution Calculator
Determine the dilution needed to prepare a stock solution.
Reconstitution Calculator
Recensioni
Recensioni dei clienti
Domande frequenti
How is this product shipped?
This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.
What does BioReagent mean?
BioReagent indicates that the product has been processed and tested for biological and biochemical applications. Specifications may include endotoxin limits, microbial controls, sterility, low DNase/RNase/protease activity, and verified biological activity where relevant.
How should this product be stored?
Store at ?20 °C, protected from light. The material is light-sensitive — keep it in its original opaque or amber container.
What documentation is provided?
Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.
Need help choosing the grade?
Our grade selection guide covers purity, stabilizer status, and application suitability for all variants in our catalog.
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Products are supplied to verified businesses, institutions, and qualified professionals for research and development use only. Not for use in humans, animals, diagnosis, or therapy.
