ADP-specific Glucokinase, CAS No.173585-07-4

CAS: 173585-07-4 Cat. No.: A1439124 Numero EC: 838-695-5

GRADE & PURITY Bioactive, Recombinant, ActiBioPure™, High Performance, EnzymoPure™, ≥90%(SDS-PAGE), Protein concentration: See COA; ≥500 U/mg protein

Accession #

Q9V2Z6

Protein Tag

N-His, C-His

Expression system

E. coli

Bioactivity

≥500 U/mg protein

Panoramica

ADP-specific Glucokinase is an ADP-dependent glucokinase (EC 2.7.1.147), with its gene derived from Pyrococcus furiosus. It exists as a dimer, with a monomer molecular weight of 47 kD. It uses ADP as the phosphate donor to catalyze the conversion of glucose to glucose-6-phosphate. This product is recombinantly expressed in E. coli, can tolerate temperatures up to 90°C, and has an optimal temperature of 50°C.

Reaction formula

Glucose + ADP →Glucose-6-phosphate + AMP  

Product Properties

Source: Expressed in E. coli

Label: N-His & C-His

Purity: ≥ 90% (analyzed by SDS-PAGE)

Molecular weight: 100 kDa

Buffer: 20 mM Tris-HCl, 500 mM NaCl, 10% Glycerol, pH 7.5.

Activity: ≥500 U/mg protein

Unit definition: One unit of enzyme activity is defined as the amount of enzyme required to consume 1.0 μmol of ADP per minute under conditions of 50°C and pH 7.5.

Usage Instructions

1. Add the substrate and 10 mM MgCl₂ to the reaction system; adjust the pH to 7.5 with appropriate buffer salts, then add a suitable amount of ADP-specific Glucokinase (as needed for the reaction);

2. After mixing, incubate the sample at 50°C for 4 hours or overnight.

【Note】 When scaling up the reaction system, adjust the incubation time or enzyme dosage according to actual conditions; low-speed shaking can be used for mixing.

【Note】 Most common biological buffers (e.g., Tris or PBS) are applicable. Buffers outside this pH range (e.g., acetate buffer) may be usable, but the incubation time or enzyme dosage needs to be optimized based on actual conditions.

Precautions

For your safety and health, wear a lab coat and disposable gloves during operation. This product is for research use only!

Specifications

Product Name

ADP-specific Glucokinase, CAS No.173585-07-4

Grado

ActiBioPure™, Bioactive, High Performance, Recombinant, EnzymoPure™

Specifiche e purezza

Bioactive,Recombinant,ActiBioPure™,High Performance,EnzymoPure™,≥90%(SDS-PAGE),Protein concentration: See COA; ≥500 U/mg protein

Meccanismi biochimici e fisiologici

Catalyzes the ADP-dependent phosphorylation of D-glucose to D-glucose 6-phosphate and glucosamine to glucosamine 6-phosphate. Can also use CDP as the phosphoryl group donor and D-1,5-anhydroglucitol as the phosphoryl group acceptor.

Bioattività

≥500 U/mg protein

Numero d'ordine

Q9V2Z6

Peso molecolare previsto

100 kDa

CAS

173585-07-4

Tipo di molecola

Enzima

Stoccaggio e spedizione

Concentrazione

Protein concentration: See COA; ≥500 U/mg protein

Condizioni di conservazione di stoccaggio

Store at -20°C,Avoid repeated freezing and thawing

Spedito in

Ice chest + Ice pads

Stabilità e conservazione

Store at -20℃ long term (18 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Definizione dell'unità

The amount of enzyme required to consume 1.0 μmole of ADP per minute at 50 ºC and pH 7.5.

Domande frequenti

What is the purity of this product, and how is it determined?

This product is supplied at ≥90% purity, determined by SDS-PAGE. Lot-specific values are stated on the Certificate of Analysis.

What does Bioactive mean?

Bioactive indicates that the product has been processed and tested for biological and biochemical applications. Specifications may include endotoxin limits, microbial controls, sterility, low DNase/RNase/protease activity, and verified biological activity where relevant.

What does Recombinant mean?

Recombinant is a defined analytical purity tier suitable for workflows requiring a specific validated property. It specifies a minimum assay percentage, controlled limits on common impurities (water, heavy metals, residue on ignition), and lot-to-lot consistency. Recombinant is one of several purity tiers — choose based on the sensitivity and regulatory requirements of your method.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What are the CAS number, molecular formula and molecular weight?

The CAS Number is 173585-07-4.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.