AF647-NHS ester triTEA (6C-Linker) - BioReagent, Biological Stain, for Fluorescence analysis, for Microscopy, ≥98%(HPLC) , CAS No.407627-61-6

CAS: 407627-61-6 Cat. No.: A1455333 Formula: C₅₈H₉₄N₆O₁₆S₄ Peso molecolare: 1259.66

GRADE & PURITY BioReagent, Biological Stain, for Fluorescence analysis, for Microscopy, ≥98%(HPLC)

Storage

Protected from light,Store at -20°C,Desiccated,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Application

Cell Staining, Fluorescence Analysis, Protein/Antibody Labeling

Panoramica

AF647 NHS Ester is a far-red fluorescent dye with good photostability. Its fluorescence signal decays slowly during long-duration experiments and maintains stable fluorescence for extended periods, which is beneficial for long-term tracking and observation of biomolecules. It has a high fluorescence quantum yield. The activated ester group of this dye can react with amino groups (primarily primary amines) on target biomolecules to form stable amide bonds, thereby labeling biomacromolecules with AF647 fluorescent moieties.

Spectral properties: 

Excitation/absorption maximum: 651 nm 

Emission maximum: 671 nm 

Extinction coefficient (ε): 265000 

Fluorescence quantum yield (Φ): 0.33 

Precautions for Fluorescent Products: 

1. Activated esters are prone to hydrolysis. After taking out from the refrigerator, allow the vial to warm to room temperature in a dry environment before opening. Since the reagent quantity is small, gently tap the vial several times before opening to ensure all powder falls to the bottom.

2. It is recommended to prepare the stock solution fresh at first use. Use the dissolved reagent as soon as possible to guarantee experimental performance.

3. Fresh anhydrous DMSO is required for dissolution. Water contamination will cause hydrolysis of the activated ester and compromise experimental results.

4. The prepared stock solution is susceptible to hydrolysis upon exposure to water. If not fully consumed in one experiment, aliquot the solution (e.g., 5–8 μL per tube), seal with parafilm, wrap with aluminum foil, place in a well-sealed plastic bag containing desiccant, and store hermetically at ≤ -20°C protected from light.

5. It is recommended to optimize cell density, dye/probe concentration and incubation time in preliminary tests before formal experiments to determine optimal experimental conditions.

Specifications

Specifiche e purezza

BioReagent, Biological Stain, for Fluorescence analysis, for Microscopy, ≥98%(HPLC)

Stabilità e conservazione

Store at -20℃ long term (24 months). Store in the dark. Desiccated. Avoid freeze/thaw cycle.

Condizioni di conservazione di stoccaggio

Protected from light,Store at -20°C,Desiccated,Avoid repeated freezing and thawing

Spedito in

Ice chest + Ice pads

Questo prodotto richiede spedizione a catena fredda. I servizi di terra e altri servizi economici non sono disponibili.

Grado

Biological Stain, BioReagent, per l'analisi a fluorescenza, per la microscopia

Purezza

≥98%(HPLC)

Nomi e identificatori

• CAS alternativo: 407627-60-5 (free base)
• Peso molecolare: 1259.66

Proprietà chimiche e fisiche

• Solubilità: Soluble in DMSO