AGX51 - 10mM in DMSO , CAS No.330834-54-3

CAS: 330834-54-3 Cat. No.: A654898 Formula: C₂₇H₂₉NO₄ Peso molecolare: 431.52

GRADE & PURITY 10mM in DMSO

Storage

Store at -80°C

Shipped In

Dry ice packs + Cold packs

Panoramica

AGX51 is a first-in-class pan- Id (inhibitors of DNA-binding/differentiation proteins) antagonist and degrader. AGX51 inhibits the Id1-E47 interaction, leading to ubiquitin-mediated degradation of Ids, cell growth arrest, and reduces viability. AGX51 inhibits the TNBC cell lines with IC 50 s of nearly 25 μM. AGX51 can be used for the research of cancer

In Vitro

AGX51 (0-80 μM; 24 h) decreases ID1 protein levels in 4T1 cells. ?\nAGX51 (40 μM; 0-72 h) decreases ID1 levels protein with a 40 μM concentratio in 4T1 cells. ?\nAGX51 (40 μM; 24 h) influences 4T1 cells , ER+, HER2+, TNBC and three breast cancer PDX cell ines. ?\nAGX51 (0-80 μM; 24-48 h) influences cell cycle of 4T1 cells. ?\nAGX51 (40 μM; 4-24 h) influences phospho-histone H3 levels in 4T1 cells. ?\nAGX51 (40 μM; 24 h) influences ROS levels in 4T1 cells. MCE has not independently confirmed the accuracy of these methods. They are for reference only. Western Blot AnalysisCell Line: 4T1 cells Concentration: 0, 5, 10, 20, 40 and 80 μM Incubation Time: 24 hours Result: Decreased ID1 protein levels starting at a concentration of 40 μM in 4T1 cells. Western Blot AnalysisCell Line: 4T1 cells Concentration: 40 μM Incubation Time: 0, 2, 4, 8 , 12, 24, 48 and 72 hours Result: Decreased ID1 protein levels starting at 4 h, while until 24 h ID1 protein completely loss. Cell Viability AssayCell Line: 4T1 cells, HMLE RAS Twist , MDA-MB-157, MDA-MB-436, MDA-MB-231, MDA-MB-453, BT-474, MDA-MB-361, SK-BR-3, MCF-7, T47-D, PDX-BR7, PDX-IBT and PDX-BR11 Concentration: 40 μM Incubation Time: 24 hours Result: Inhibited 4T1, HMLE RAS Twist , MDA-MB-157, MDA-MB-436, SK-BR-3, MCF-7, PDX-BR7, PDX-IBT and PDX-BR11 cell lines with IC 50 s of 26.66, 8.7, 22.28, 30.91, 36.55, 60, 10.89, 11.97 and 18.56 μM, respectively. Cell Cycle AnalysisCell Line: 4T1 cells Concentration: 40 μM Incubation Time: 24 and 48 hours Result: Affected cell cycle of 4T1 cells with a G0/G1 phase accumulation. Cell Viability AssayCell Line: 4T1 cells Concentration: 40 μM Incubation Time: 4 and 24 hours Result: Reduced phospho-histone H3 levels in 4T1 cells. Cell Viability AssayCell Line: 4T1 cells Concentration: 40 μM Incubation Time: 24 hours Result: Increased ROS level of 4T1 cells and indicated ROS production is a main mechanism of cell killing.

In Vivo

AGX51 (50 mg/kg; i.p. twice a day for 4 weeks) inhibits lung metastasis . ?\nAGX51 (15 mg/kg; i.p. twice a day for 3 weeks) exibits anti-tumor activity with autochronous cancer . MCE has not independently confirmed the accuracy of these methods. They are for reference only. Animal Model: Balb/c mice with luciferase-labeled 4T1 cells Dosage: 50 mg/kg Administration: Intraperitoneal injection; 60 mg/kg twice a day; for 4 weeks Result: Inhibited lung metastasis development. Animal Model: A/J mice with AOM colon tumor model Dosage: 15 mg/kg Administration: Intraperitoneal injection; 15 mg/kg twice a day; for 3 weeks Result: Dreased the colon tumors and exhibited anti-tumor activity in AOM colon tumor mice.

IC50& Target:IC50: 26.66 μM (4T1), 8.7 μM (HMLE RAS Twist), 22.28 μM (MDA-MB-157), 30.91 μM (MDA-MB-436), 36.55 μM (SK-BR-3), 60 μM (MCF-7), 10.89 μM (PDX-BR7), 11.97 μM (PDX-IBT) , 18.56 μM (PDX-BR11)

Specifications

Specifiche e purezza

10mM in DMSO

Meccanismi biochimici e fisiologici

AGX51 is a first-in-class pan- Id (inhibitors of DNA-binding/differentiation proteins) antagonist and degrader. AGX51 inhibits the Id1-E47 interaction, leading to ubiquitin-mediated degradation of Ids, cell growth arrest, and reduces viability. AGX51 inhi

Condizioni di conservazione di stoccaggio

Store at -80°C

Spedito in

Dry ice packs + Cold packs

Questo prodotto richiede spedizione a catena fredda. I servizi di terra e altri servizi economici non sono disponibili.

Tipo di azione

ANTAGONIST

Nomi e identificatori

• Peso molecolare: 431.52

Domande frequenti

How should this product be stored?

Store at ?80 °C. Ultra-low temperature storage is required to maintain the specified shelf life.

How is this product shipped?

This product ships frozen with dry ice and cold packs. Unpack on arrival and transfer it to the storage condition stated above; handle dry ice with insulated gloves in a ventilated area.

What are the CAS number, molecular formula and molecular weight?

The CAS Number is 330834-54-3, the molecular formula is C27H29NO4, and the molecular weight is 431.52 g/mol.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.