AMF Mouse mAb - Primary antibody, specific to AMF, IgG1

Cat. No.: Ab088587
AVAILABLE TO ORDER
GRADE & PURITY Carrier Free ? Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays. Validated ? Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated. ExactAb™ ? ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications. 1.0mg/mL
Application
ELISA, IF/ICC, IHC, WB
Host species
Mouse
Reactive species
Human
Isotype
Mouse IgG1
Clone Number
1B7D7
Conjugation
Unconjugated
 ·  off list, applied to all prices below.
Size
Status
Price
Qty
10μl
Ab088587-10μl
4
$79.90
50μl
Ab088587-50μl
2
$259.90
100μl
Ab088587-100μl
1-2 wks(?)
Item is derived from our semi-finished stock and is processed in 1-2 weeks.
$439.90
1ml
Ab088587-1ml
1-2 wks(?)
Item is derived from our semi-finished stock and is processed in 1-2 weeks.
$3,999.90
Enter a quantity for the sizes you want to add.
🧪

Why this grade

ExactAb™, Validated, Carrier Free, 1.0mg/mL Carrier Free,ExactAb™,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.

🌡

Storage & shipping

Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.

📋

Quality documents

SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.

📚

Literature proof

Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.

Overview

Besides it's role as a glycolytic enzyme, mammalian GPI can function as a tumor-secreted cytokine and an angiogenic factor (AMF) that stimulates endothelial cell motility. GPI is also a neurotrophic factor (Neuroleukin) for spinal and sensory neurons.

Specifications

Product Name
AMF Mouse mAb - Primary antibody, specific to AMF, IgG1
Synonyms
AMF antibody | Aurocrine motility factor antibody | Autocrine motility factor antibody | DKFZp686C13233 antibody | EC 5.3.1.9 antibody | G6PI_HUMAN antibody | Glucose phosphate isomerase antibody | Glucose-6-phosphate isomerase antibody | GNPI antibody |
Specifications & Purity
ExactAb™, Validated, Carrier Free, 1.0mg/mL
Host species
Mouse
Specificity
AMF
Immunogen
Purified recombinant fragment of human GPI(AA:1-558) expressed in E.Coli.
Positive Control
WB: Hep G2, A431, K562, HeLa, A549, MCF-7 cell lysates. ICC/IF: L-02 cells. IHC: Human Kidney tissues.
Conjugation
Unconjugated
Grade
Carrier Free, ExactAb™, Validated
Isotype
IgG1
Light Chain Type
kappa
SDS-PAGE
150kDa
Purification Method
Protein G purified
Concentration
1.0mg/mL
Storage and Shipping
Shape
Liquid
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Images

AMF Mouse mAb (Ab088587) - Western Blot
All lanes: AMF Mouse mAb (Ab088587) at 1/1000 dilution

Samples: Lysates at 20 µg per lane

Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/10000 dilution

Predicted band size: 63 kDa
Observed band size: 57 kDa

AMF Mouse mAb (Ab088587) - ICC/IF
AMF Mouse mAb (Ab088587) at 1/1000 dilution staining AMF in L-02 cells by Immunocytochemistry/ Immunofluorescence. An AF488 conjugated Goat polyclonal to mouse IgG1 was used as secondary antibody. Green staining in image show positive staining with AMF Mouse mAb, actin filaments were stained red with DY-554 phalloidin and nuclei stained blue with DRAQ5 fluorescent DNA dye.

AMF Mouse mAb (Ab088587) - IHC
Immunohistochemical analysis of paraffin-embedded human Kidney tissues using AMF Mouse mAb (Ab088587) .

Application
ApplicationDilution info
WB

1/500-1/2000

IF/ICC

1/200-1/1000

ELISA

1/10000

IHC

1/200-1/1000

Documentation

📋 Safety Data Sheet (SDS)

Comprehensive hazard, handling, storage, and regulatory compliance document.

Download SDS →

✅ Certificate of Analysis (COA)

Lot-specific quality data. Enter your lot number to retrieve the exact COA.

Look up COA →

📊 Datasheet

Quick-reference summary of product specifications and applications.

View datasheet →

🔬 Specification Sheet

Full quality attributes and acceptance criteria for this grade.

View spec sheet →

Advanced Data

Associated Targets(Human)
GPI Tbio Glucose-6-phosphate isomerase (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)
Mechanisms of Action
Certificates(CoA,COO,BSE/TSE and Analysis Chart)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

2 results found

Lot NumberCertificate TypeDateItem
ZJ23F0700700Certificate of AnalysisMay 19, 2026 Ab088587
ZJ23F0700701Certificate of AnalysisMay 19, 2026 Ab088587
Documents & Articles
Solution Calculators
Reviews

Customer Reviews

Need help choosing the grade?

Our grade selection guide covers purity, stabilizer status, and application suitability for all variants in our catalog.

View Carrier Free grade guide → View Validated grade guide → View ExactAb™ grade guide →

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