Storage buffer: Purified Water, 0.05% ProClin 300
Magnetic Particle Chemiluminescence Immunoassay is an analytical method that combines magnetic separation technology, chemiluminescence technology, and immunoassay technology. Magnetic particles, as carriers conjugated with antibodies or antigens, can capture target molecules. Due to their superparamagnetic properties, they enable multiple magnetic separations, facilitating full automation of detection instruments and accelerating testing speed. Currently, magnetic particle chemiluminescence immunoassay technology is widely used in biomedical detection due to its high sensitivity, broad detection range, and high automation.
Amine Magnetic Beads are surface-modified with amino groups (-NH₂). These groups can covalently bind to amino groups on proteins via coupling reagents such as glutaraldehyde, forming Schiff bases (imines). The resulting structure can be stabilized by reductive amination using reducing agents, yielding a stable secondary amine linkage.
检测标志物改成：Detection Marker
捕获抗体改成：Capture Antibody
磁性微球改成：Magnetic beads
检测标志物与抗体反应后改成：After reaction between detection marker and antibody
加磁场进行分离改成：Magnetic separation is applied
磁铁改成：Magnet
检测抗原改成：Detected Antigen
酶标抗体改成：Enzyme-labeled Antibody
Product Features
Diffuse Structure: The magnetic core consists of superparamagnetic γ-Fe₂O₃ nanoparticles.
Iron Oxide Content: 12% - 20% (varies depending on the specific bead type).
Particle Size: Standard sizes of 1.2 μm and 2.8 μm.
Highly Uniform Particle Size with CV < 5% between Batches: Ensures stable and reliable performance.
Excellent Dispersion and Low Detection Background: Features a uniquely designed, proprietary coating layer that exhibits extremely low non-specific adsorption.
Production Capacity of 2 kg per Batch: Meets the routine demands of chemiluminescence customers.
High Stability: No oxidation/discoloration or magnetic core leakage occurs after long-term storage.
Instructions for Use
1. Conjugation Conditions
(1) PBS (pH 7.4) is recommended as the conjugation buffer. Buffers containing primary amine groups (e.g., Tris, glycine) should be avoided.
(2) For IgG antibodies, the recommended conjugation ratio is IgG:Beads = 10–30 μg:1 mg. However, due to the diversity of proteins, screening different ratios is advised for optimal results.
(3) After conjugation, blocking can be performed using small molecules containing amino groups (e.g., ethanolamine). Additionally, blocking with proteins such as BSA can be considered as needed.
2. Precautions
(1) Before use, thoroughly vortex the magnetic beads to ensure a uniform suspension.
(2) Drying or freezing can cause bead aggregation and may affect the activity of surface functional groups. Please adhere to the recommended storage conditions.
3. Recommended Conjugation Protocol
Item
| Details
|
Conjugation Buffer
| 10 mM PBS, pH 7.4
|
Glutaraldehyde Solution
| Diluted to 0.5% with pure water; prepare fresh
|
Sodium Cyanoborohydride Solution
| 5 M Sodium Cyanoborohydride (dissolved in 1 M NaOH)
|
Blocking Buffer
| 0.1 M Ethanolamine, pH 7.4
|
Storage Buffer
| PBS containing 0.5% BSA, pH 7.4 |
4. Conjugation Method
(1) Thoroughly vortex the amino magnetic beads to suspend evenly, and transfer 10 mg to a centrifuge tube.
(2) Place the tube on a magnetic rack for 1–2 minutes and remove the supernatant.
(3) Add 1.0 mL of Conjugation Buffer, vortex to disperse, and remove the supernatant as in step (2).
(4) Repeat the wash with Conjugation Buffer once more.
(5) Add 1.0 mL of Glutaraldehyde Solution, vortex to disperse. Maintain suspension with mixing and react at 25°C for 1 hour protected from light. Remove the supernatant as in step (2).
(6) Add 1.0 mL of Conjugation Buffer, vortex to disperse, and remove the supernatant as in step (2).
(7) Repeat the wash with Conjugation Buffer twice.
(8) Add 1.0 mL of Conjugation Buffer, vortex to disperse. Then add 200 μg of antibody, vortex to disperse. Maintain suspension with mixing and react at 25°C for 2 hours protected from light.
(9) Add 10 μL of Sodium Cyanoborohydride Solution. Maintain suspension with mixing and incubate at 25°C for 1 hour. Remove the supernatant as in step (2).
(10) Add 1.0 mL of Blocking Buffer, vortex to disperse. Maintain suspension with mixing and incubate at room temperature for 30 minutes. Remove the supernatant as in step (2).
(11) Repeat the wash with Storage Buffer three times.
(12) Add 1.0 mL of Storage Buffer, vortex to mix, resulting in a magnetic bead suspension at 10 mg/mL. Store at 2–8°C.
ZJ26F0231884