GRADE & PURITYBioReagent?BioReagent grade — tested suitable for life-science and molecular-biology use. Use for cell culture, assays, and biochemical work needing biological compatibility.
BioReagent BioReagent for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at 2-8°C,Protected from light Ships Wet ice Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Panoramica
Amylase (AMS), also known as 1,4-α-D-glucan hydrolase, is a general term for a class of enzymes capable of catalyzing the hydrolysis of starch and glycogen. Based on the substrate used for detection, its assay methods are primarily divided into two categories: one employs natural starch as the substrate (such as the iodine-starch method adopted by this kit), and the other utilizes defined substrates (such as maltopentaose or 4-nitrophenyl maltoside (4-NP-G)).
This kit is based on the classic iodine-starch colorimetric principle. The α-amylase in the sample catalyzes the hydrolysis of the α-1,4-glycosidic bonds in starch molecules, producing glucose, maltose, dextrins, and other products. In the reaction system, iodine solution binds to the unhydrolyzed starch to form a blue complex. The higher the α-amylase activity, the less starch remains, resulting in a lighter blue color. By measuring the absorbance at 660 nm using a microplate reader and comparing it with a blank control, the amylase activity units in the sample can be calculated.
It is suitable for detecting endogenous amylase activity in various biological samples such as cell or tissue lysates/homogenates, plasma, serum, and urine. This product is intended for research use only and is not suitable for clinical diagnosis or other purposes.
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What does BioReagent mean?
BioReagent indicates that the product has been processed and tested for biological and biochemical applications. Specifications may include endotoxin limits, microbial controls, sterility, low DNase/RNase/protease activity, and verified biological activity where relevant.
How should this product be stored?
Store at 2–8 °C, protected from light. The material is light-sensitive — keep it in its original opaque or amber container.
How is this product shipped?
This product ships chilled on wet ice. Unpack on arrival and transfer it to the storage condition stated above.
What documentation is provided?
Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.
Need help choosing the grade?
Our grade selection guide covers purity, stabilizer status, and application suitability for all variants in our catalog.
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Products are supplied to verified businesses, institutions, and qualified professionals for research and development use only. Not for use in humans, animals, diagnosis, or therapy.
