Aniline Blue Stain Solution (2%) - 2%

Cat. No.: A1516049

GRADE & PURITY 2%

Synonyms

Aniline Blue Staining Solution

Storage

Room temperature

Shipped In

Normal

Application

Cell Staining

Panoramica

In a narrow sense, connective tissue contains three types of fibers: collagen fibers, reticular fibers, and elastic fibers. Among these, collagen fibers are the most widely distributed and abundant fiber type. Masson’s Trichrome Staining, also known as Masson staining, is the most classic method for connective tissue staining and an authoritative and standard technique for collagen fiber staining. Trichrome staining generally stains nuclei and selectively demonstrates collagen fibers and muscle fibers. The staining principle is related to the molecular size of anionic dyes and tissue permeability: Molecular size is reflected by molecular weight. Small molecules easily penetrate dense tissues with low permeability, whereas large molecules can only enter loose tissues with high permeability. Since light green or aniline blue has a large molecular weight, muscle fibers appear red and collagen fibers appear green (light green) or blue (aniline blue) after Masson staining. This method is mainly used to distinguish collagen fibers from muscle fibers.

Aniline Blue Staining Solution (2%) consists mainly of aniline blue and weak acid. It is often used in combination with Ponceau Fuchsin Staining Solution to stain collagen fibers. This reagent is for research use only. Not for clinical diagnosis or other purposes.

Operating Procedures (For Reference Only):

1. Perform the operation according to the specific experimental requirements.

2. Generally, immerse and stain for 1–2 minutes; the duration can be adjusted appropriately according to the staining results.

Precautions:

1. Section deparaffinization should be as thorough as possible.

2. For your safety and health, wear laboratory coat and disposable gloves during operation.

Specifications

Sinonimi

Aniline Blue Staining Solution

Specifiche e purezza

2%

Stabilità e conservazione

Store at room temperature long term (12 months).

Condizioni di conservazione di stoccaggio

Room temperature

Spedito in

Normal

Product Line

biological_products

Application Protocols

Tested applications, validated protocols, and recommended dilutions are not specified for this item; refer to CoA/Spec Sheet and SDS.

• General guidance (non-validated, literature-based; not item specification): Users typically apply the stain to fixed specimens, incubate until the desired intensity is reached, rinse gently, and mount for brightfield or fluorescence imaging depending on the medium. Optimize time, pH, and mounting medium for your specimen.

Because no manufacturer-validated procedures are provided for this SKU, users should establish laboratory-specific protocols and include appropriate controls.

Biological Roles

This section addresses what the stain reveals in biological samples (literature/general context; not item specification).

• Targeted structures: Aniline blue preferentially associates with polysaccharide-rich matrices, notably β-1,3-glucan (callose) in plants and glucan/chitin components of fungal cell walls. The dye’s sulfonated triphenylmethane framework enables ionic and noncovalent interactions with these anionic or hydrogen-bonding-rich substrates.
• Plant biology applications: Detection of callose deposition in response to stress, plasmodesmata regulation studies, and visualization of pollen tube growth. Under appropriate excitation and mounting, aniline blue can display strong fluorescence of callose, improving sensitivity relative to brightfield.
• Tissue histology: In multicomponent stains (e.g., trichrome-like protocols), aniline blue provides contrast for collagenous extracellular matrix and other connective tissue components, aiding morphometric assessments.
• Microbial structures: Staining of fungal hyphae and spores facilitates localization within host tissues or materials.

Notes and caveats

• Signal depends on fixation, pH, ionic strength, and mounting medium; control experiments with enzymatic digestion (e.g., β-1,3-glucanase) can verify specificity for callose/glucans.
• Avoid clinical or diagnostic interpretation unless validated in the appropriate regulatory framework; this product is labeled for research use only.
• Photostability and quenching can vary; minimize prolonged high-intensity illumination to preserve fluorescence where applicable.

Buffer Applications

Not a buffering reagent. This SKU is a ready-to-use aqueous stain and is not typically employed to control pH or ionic strength.

• If your protocol specifies a particular pH, adjust the rinse or mounting medium with an appropriate buffer (e.g., phosphate or citrate, selected based on staining chemistry) prepared separately.
• Maintain consistent pH across runs, as dye selectivity and intensity may vary with acidity.

Green Alternatives

This product is already water-based, which aligns with green chemistry principles by minimizing volatile organic compounds and flammability.

Comparison (literature/general; not item specification)

• Current product: 2% aqueous aniline blue
  • Pros: water as solvent (low VOC), ready-to-use (reduces handling and dye dust), consistent performance, minimal fire risk.
  • Cons: dye-containing aqueous waste requires appropriate disposal; potential persistence of triphenylmethane dyes in the environment.
• Powder dye made up in-lab
  • Pros: reduced shipping mass and packaging; customizable pH and additives.
  • Cons: dust exposure risk; variability between preparations; potential for spills and overdosing; operator time.
• Alternative stains for similar purposes
  • Calcofluor white (fluorescent brightener): strong β-glucan binding and high sensitivity; however, often supplied as powder/concentrate and may require more stringent hazard controls.
  • Alcian blue or toluidine blue (for acidic polysaccharides): different selectivity profiles; verify compatibility with the biological target and downstream imaging.

Greener practice tips

• Use minimal necessary bath volumes and adopt slide racks to reduce waste.
• Collect dye-containing rinses for appropriate disposal; avoid release to drains unless permitted.
• Validate shorter staining times and lower temperatures to save energy without compromising contrast.

Pharmaceutical Uses

This is a laboratory staining solution intended for research use and microscopy. It is not formulated as a pharmaceutical excipient or for clinical applications.

• Pharmacopeial status: Not specified for this item; refer to CoA/Spec Sheet.
• Manufacturing context (general): In preclinical research settings, stains like aniline blue can assist in histological evaluation of nonclinical specimens and materials; they are not components of drug products.
• Compliance note: For any regulated studies, confirm suitability, documentation (CoA, SDS), and method qualification per institutional SOPs.

No therapeutic or clinical diagnostic claims are made for this product.

Physical Properties

Item-specific facts (from Product Data)

• Physical state/appearance: Not specified for this item; refer to CoA/Spec Sheet. (Typical: clear to deep blue aqueous solution.)
• Storage conditions: Room temperature
• Shipping: Normal

Not specified for this item; refer to CoA/Spec Sheet

• Exact pH, density, refractive index, viscosity, UV-Vis λmax, residual solvents, impurities profile.

Literature/general values (for context; not item specification)

• Solvent system: water (typical for ready-to-use 2% w/v biological stains).
• Solubility: aniline blue dyes are highly soluble in water and lower alcohols due to multiple sulfonate groups.
• UV-Vis: strong absorption in the visible (blue dye; complementary absorption typically in orange-red region). Reported triphenylmethane dyes often show λmax ~ 580–620 nm, but exact values depend on composition, pH, and ionic strength.
• pH influence: staining contrast for polysaccharides can be pH-dependent; acidic media often enhance selectivity for callose and fungal cell walls.
• Volatility: negligible for solute; aqueous matrix non-flammable.

Note: Because “aniline blue” represents a dye mixture and this is a formulated 2% solution, property values vary with precise composition, counter-ions, and additives. Consult the CoA/Spec Sheet and SDS for definitive specifications applicable to this SKU.

Quality and Grades

Item-specific facts (from Product Data)

• Grade/Purity: Not specified for this item; refer to CoA/Spec Sheet.

Interpretation and practical notes (general guidance)

• This SKU is a ready-to-use 2% staining solution intended for laboratory research use. In such formulations, quality control typically focuses on dye identity/lot consistency, staining performance, absence of particulates/precipitates, and microbial control, rather than reagent-metal content or spectroscopic cutoffs.
• For imaging and morphometric work, lot-to-lot optical consistency (absorbance profile and staining intensity under defined conditions) is critical. Request the CoA to verify target absorbance at a reference wavelength, if provided, and any acceptance criteria (e.g., visual pass/fail on standard tissue sections or callose standards).
• Stabilizers/preservatives: Not specified for this item; refer to CoA/Spec Sheet. If present in similar products, low-level biocides help maintain clarity and prevent microbial growth; verify compatibility with downstream assays.
• Filtration/sterility: Unless specified as sterile, assume non-sterile. For cell-associated applications where sterility is important, filter through a 0.22 µm membrane immediately before use if compatible with your protocol.

For regulatory or method-validated environments, capture the lot CoA and, if needed, perform site qualification using your own positive control substrates.

Reaction and Applications

As a biological stain, this product is used for visualization rather than as a chemical reagent in synthesis. Key application domains (literature/general; not item specification):

• Plant biology: robust staining of β-1,3-glucan (callose) in plant tissues; often used to visualize pollen tubes, sieve plates, and wound-induced callose. Fluorescence can be observed under UV or blue excitation depending on formulation and mounting medium, enhancing contrast of callose deposits.
• Mycology and microbiology: strong affinity for fungal cell wall polysaccharides (chitin- and glucan-rich structures), aiding in hyphae and spore visualization in tissue sections and environmental samples.
• Histology and connective tissue: used as a blue counterstain in trichrome-type protocols to highlight collagenous extracellular matrix; specific selectivity depends on formulation and any accompanying mordants or acids used in the protocol.
• Materials and biofilms: visualization of polysaccharide-rich biofilms on surfaces and in cross-sections.

Practical tips (general)

• Specimen preparation: fixatives and prior treatments (e.g., clearing, enzymatic digestions) strongly influence staining; standardize fixation to achieve reproducibility.
• pH and ionic strength: acidic conditions often improve selectivity for callose; record the pH of your bath/mountant for consistency.
• Washing/differentiation: brief rinses in water or buffered solutions remove background; avoid prolonged washing that can reduce contrast.
• Mounting: fluorescence response and color stability can vary with mounting medium; test small samples when switching media.

Note: No specific validated protocols are provided for this SKU; users should optimize according to sample type and imaging modality.

Reaction Conditions

Not applicable. This product is used as a staining solution and not as a stoichiometric or catalytic reagent in chemical reactions.

• Operational considerations (general): Typical staining is carried out at ambient temperature with contact times ranging from seconds to tens of minutes depending on specimen and desired contrast. Rinsing steps are commonly performed with water or buffer. Exact parameters should be optimized per sample type.

No SKU-specific reaction conditions, catalysts, or yields are relevant.

Safety and Handling

Item-specific hazard data

• Signal word / H-statements / GHS classification / Pictograms: Not specified for this item; refer to SDS.

General safety considerations for aqueous aniline blue staining solutions (literature/general; not item specification)

• Likely hazards: may cause skin/eye irritation and staining; ingestion and inhalation should be avoided. Triphenylmethane dyes can be harmful if swallowed; avoid contact with mucous membranes.
• PPE: lab coat, nitrile gloves, and splash goggles; use in a well-ventilated area or fume hood if aerosols/mists may form.
• First aid (overview; defer to SDS):
  • Skin: wash with soap and water; remove contaminated clothing.
  • Eyes: rinse cautiously with water for several minutes; remove contact lenses if easy; seek medical attention for persistent irritation.
  • Ingestion: rinse mouth; do not induce vomiting; seek medical advice.
  • Inhalation: move to fresh air; seek medical attention if symptoms persist.
• Storage incompatibilities: keep away from strong oxidizers and strong acids/bases that could alter dye performance; avoid contact with reactive bleaching agents.
• Spill response: contain with absorbent, collect in appropriate waste; area may remain visibly stained—decontaminate surfaces promptly.
• Waste: dispose according to institutional and local regulations for dye-containing aqueous waste.

Always consult the product’s SDS for authoritative, SKU-specific hazard, exposure limits, and response measures.

Solvent Selection

This product is a 2% aqueous staining solution, so classical solvent selection is generally not applicable.

• Matrix: water (literature/general). Water provides excellent wetting of biological specimens and minimizes VOC emissions.
• Miscibility: completely miscible with water; may also mix with lower alcohols (e.g., ethanol) if protocol adaptations require faster drying or differentiated staining, but verify compatibility with your method.
• Polarity/dielectric: high dielectric constant (water ~78 at 25 C, literature), favoring ionic dye solubility and stable coloration.
• When to choose: use this ready-to-use solution to avoid weighing dyes, pH adjustment, and variability. Choose powder or concentrates if you need customized pH, counterstain blends, or solvent composition.

Comparison (general)

• Aqueous aniline blue vs alcoholic stains: aqueous systems are safer, less flammable, and gentler on tissues; alcoholic systems may dehydrate specimens and accelerate drying but can alter staining selectivity.

If your workflow demands non-aqueous mounting or rapid dehydration, perform staining in aqueous solution followed by graded alcohols and clearing agents per your histology protocol.

Storage and Reconstitution

Item-specific facts (from Product Data)

• Storage conditions: Room temperature
• Shipped in: Normal

Use and handling

• Format: Ready-to-use 2% aqueous staining solution; no reconstitution required.
• Protect from contamination: Keep container tightly closed. To avoid microbial contamination and particulate introduction, do not pipette from the original bottle over open specimens; decant working aliquots instead.
• Light sensitivity: Triphenylmethane dyes can fade with prolonged light exposure (literature/general). Store in the original, light-resistant container or in a dark cabinet to maximize shelf life.
• Freeze–thaw: Not applicable; avoid freezing unless specified on the CoA, as precipitation or performance changes may occur upon thawing.

Stability

• Shelf life and exact stability window: Not specified for this item; refer to CoA/Spec Sheet.

Allow the solution to equilibrate to room temperature before use if stored cool, and mix gently to ensure homogeneity. Discard if precipitates, microbial growth, or unexpected color changes are observed.

Structure and Identity

Item-specific facts (from Product Data)

• Product: Aniline Blue Stain Solution (2%)
• SKU: A1516049
• Concentration: 2% (w/v) dye solution
• CAS / CID / InChIKey / SMILES: Not specified for this item; refer to CoA/Spec Sheet.
• Molecular formula and molecular weight: Not specified for this item; refer to CoA/Spec Sheet.

Literature/general description (for context; not item specification)

• “Aniline blue” in microscopy commonly refers to a mixture of sulfonated triphenylmethane dyes (e.g., methyl blue, aniline blue WS) that are highly water-soluble and intensely blue.
• Structural features: polyaromatic triphenylmethane core bearing multiple sulfonate groups (–SO3−) to confer water solubility; typically supplied as sodium sulfonate salts. No defined stereocenters; mixtures may contain isomeric sulfonation patterns.
• 2D structural motif (verbal): three benzene rings joined via a central sp3 carbon (triarylmethyl) with auxochromic amino groups and aromatic sulfonates; extended conjugation provides strong blue color and high molar absorptivity.

Use domain

• Category Path: 全部 / 可售 / 生命科学
• Research Use Note: For research use only.

Synthetic Utility

Not a synthetic building block or reagent. This SKU is a formulated staining solution and is not typically used to perform chemical transformations.

• If dye incorporation into materials (e.g., polymer visualization) is desired, it functions as a tracer rather than a reactive component.
• For chemical synthesis involving triphenylmethane dyes or sulfonated aromatics, use dedicated reagent-grade powders with defined identity and purity, not ready-to-use staining solutions.

Target Specificity

Not an antibody or affinity reagent. No clone, isotype, or species reactivity applies.

• General note (literature context): In microscopy, aniline blue exhibits preferential staining of β-1,3-glucans (callose) and other polysaccharide-rich matrices. However, specificity can vary with pH and formulation; include appropriate positive/negative controls.

Item-specific target details are not provided for this SKU.