Arpc2 Mouse Pre-designed siRNA Set A

Cat. No.: A1466799

Storage

Store at -20°C

Shipped In

Ice chest + Ice pads

Panoramica

Arpc2 Mouse Pre-designed siRNA Set A contains three designed siRNAs for Arpc2 gene (Mouse), as well as a negative control, a positive control, and a FAM-labeled negative control. Components Arpc2 siRNA-1: 5 nmol (HPLC) Arpc2 siRNA-2: 5 nmol (HPLC) Arpc2 siRNA-3: 5 nmol (HPLC) siRNA Negative Control: 5 nmol (HPLC) FAM-labeled siRNA Negative Control: 5 nmol (HPLC) GAPDH siRNA Positive Control:5 nmol (HPLC)

Specifications

Condizioni di conservazione di stoccaggio

Store at -20°C

Spedito in

Ice chest + Ice pads

Questo prodotto richiede spedizione a catena fredda. I servizi di terra e altri servizi economici non sono disponibili.

Product Line

Prodotti biologici

Application Protocols

Tested applications, recommended dilutions, and protocols are provided only when explicitly listed in product data.

• Validated applications: Not specified for this item; refer to CoA/Spec Sheet.
• Recommended working concentration: Not specified for this item; refer to CoA/Spec Sheet.
• Positive control(s) and assay references: Not specified for this item; refer to CoA/Spec Sheet.

Please consult the CoA/Spec Sheet or contact technical support for assay-specific instructions for Arpc2 Mouse Pre-designed siRNA Set A.

Biological Roles

Focus: Biological context of the target gene Arpc2 in mouse (general, literature). No clinical or therapeutic claims are made.

• Gene/protein: Arpc2 encodes the p34 subunit of the Arp2/3 complex, a seven-subunit assembly that nucleates branched actin filaments.
• Cellular function: Arpc2 contributes to stabilizing the Arp2/3 core and mediates interactions with nucleation-promoting factors (e.g., WASP/Scar/WAVE). It is critical for initiating branched actin networks at membranes.
• Processes impacted: Cell migration, endocytosis, lamellipodia/filopodia dynamics, vesicle trafficking, cell shape polarity, and cytokinesis.
• Pathway positioning: Downstream of Rho-family GTPases via NPFs; integrates signals to remodel the cortical actin cytoskeleton.
• Phenotypic consequences of loss-of-function (model systems, literature): Reduced lamellipodia formation, impaired chemotaxis, altered adhesion turnover, and defects in endocytic internalization.
• Experimental utility of siRNA: Transient knockdown allows time-resolved interrogation of cytoskeletal remodeling without permanent genomic alteration.

Item-specific biological claims (e.g., observed knockdown efficiency, sequence coverage, isoform specificity): Not specified for this item; refer to CoA/Spec Sheet.

Buffer Applications

This product is not a classical buffering agent. However, correct buffer selection is critical for siRNA stability and performance (general, literature guidance).

• Stock preparation buffers: RNase-free water or 1× TE (10 mM Tris–HCl, 1 mM EDTA, pH 7.5–8.0). TE can enhance long-term stability by chelating divalent cations.
• Working solutions: Dilute into serum-free medium for complexation with transfection reagent, then replace with complete medium after 4–24 h depending on reagent guidance.
• Ionic strength: Moderate monovalent salt (e.g., 50–150 mM NaCl/KCl) supports duplex stability; high Mg2+ can catalyze RNA cleavage—avoid free Mg2+ in storage buffers.
• RNase control: Use RNase-free plastics, filtered tips, and treat surfaces with RNase decontaminants. Include RNase inhibitor only where compatible with downstream applications; typically unnecessary for standard cell transfection.

Item-specific buffer composition recommendations: Not specified for this item; refer to CoA/Spec Sheet.

Green Alternatives

For nucleic-acid reagents, environmental considerations emphasize aqueous systems, minimized hazardous solvents, and cold-chain efficiency.

Greener practice recommendations (general):

• Use aqueous buffers (RNase-free water or TE) exclusively; avoid chlorinated or high-VOC solvents in any ancillary steps.
• Prefer lipid-based transfection protocols that operate in serum-free media for short durations, then restore complete media to reduce waste.
• Scale experiments to minimize reagent excess and plastic consumables; consider low-retention tips and microvolume formats.
• Consolidate shipments where possible; maintain cold-chain only as required to reduce packaging waste. This item is shipped in an ice chest with ice pads (item-specific fact below).

Comparison (general context):

• Electroporation vs lipid transfection: Electroporation reduces chemical waste but can consume higher electrical energy and may decrease cell viability in some lines; lipids produce plastic waste from consumables but often require milder handling.

Item-specific notes:

• Shipped in: Ice chest + ice pads (provided product data).
• Storage: −20 °C (provided product data). Optimize inventory management to limit repeat shipments and reduce cold-pack waste.

Pharmaceutical Uses

This is a research-use siRNA reagent and is not intended for human or veterinary use and not for diagnostic or therapeutic applications.

Formulation/CMC context (general, literature):

• Research siRNA is typically supplied for in vitro applications. Pharmaceutical-grade oligonucleotides (not implied for this item) require defined GMP synthesis, stringent impurity profiles, endotoxin control, and stability-indicating methods.
• Excipient systems for clinical formulations (e.g., lipid nanoparticles, buffered sucrose) are beyond the scope of this product and are not claimed here.

Item-specific pharmacopeial status, excipients, and GMP statements: Not specified for this item; refer to CoA/Spec Sheet.

Physical Properties

Item-specific physico-chemical specifications are not provided in the listing.

• Appearance: Not specified for this item; refer to CoA/Spec Sheet.
• Solubility: siRNA is readily soluble in RNase-free water or TE buffer (literature). Typical stock concentrations: 10–100 µM (literature). Item-specific recommended diluent/concentration: Not specified for this item; refer to CoA/Spec Sheet.
• pH stability (general, literature): siRNA is stable near neutral pH (≈7–8); prolonged exposure to alkaline conditions can cause strand scission. Acidic conditions may promote depurination/deamination over time.
• Thermal behavior (general, literature): Duplex melting temperature (Tm) depends on sequence/length and salt; typical 19–21mer siRNA Tm often 60–85 °C in 100 mM monovalent salt, but exact Tm is sequence-dependent. For this item: Not specified; refer to CoA/Spec Sheet.
• Hygroscopicity: Lyophilized oligos can be mildly hygroscopic; minimize ambient exposure (general).
• UV characteristics (general): siRNA absorbs at 260 nm; extinction coefficient is sequence-dependent. For this item: Not specified; refer to CoA/Spec Sheet.

Do not treat any of the above as product specifications. Where numerical values are essential to your workflow (e.g., extinction coefficient, nmol amount, concentration), consult the item’s CoA/Spec Sheet.

Quality and Grades

Grade/Purity for this item is not stated in the listing.

• Declared grade/purity: Not specified for this item; refer to CoA/Spec Sheet.

Context for siRNA quality (general, literature):

• Research-grade siRNA is commonly supplied desalted and duplexed, often QC’d by MALDI-TOF or ESI-MS for mass confirmation and by UV spectrophotometry for quantitation (A260). Duplex integrity may be verified by native PAGE.
• Higher stringency options (not implied for this item unless stated): HPLC or PAGE purification, additional RNase-free certification, and endotoxin testing for sensitive cell systems.
• Stabilizer/format: Some suppliers add RNase-free salts/buffers, or provide lyophilized duplexes for improved storage. For this item, format, stabilizers, and residual solvents/salts are Not specified; refer to CoA/Spec Sheet.

What the grade means in practice (general):

• For routine cell culture knockdown, research-grade duplexes with confirmed mass and OD are typically sufficient.
• For in vivo or primary cell applications, researchers often seek ultra-pure, low-endotoxin materials and defined chemical modifications; such attributes are not claimed here and must be verified in the CoA/Spec Sheet.

Recommendation: Verify the nmol amount, purification method, residual salt levels, endotoxin (if relevant), and OD260 data on the CoA before experimental planning.

Reaction and Applications

This product is intended for RNA interference (RNAi) experiments to reduce expression of the mouse Arpc2 gene in cultured cells or ex vivo systems. No chemical synthesis or bench organic reactions are foreseen for this reagent.

Applications (general, literature):

• Target validation: Assess phenotypes arising from transient Arpc2 knockdown, supporting studies of the Arp2/3 complex in actin nucleation and cell motility.
• Pathway analysis: Dissect cytoskeletal remodeling, lamellipodia formation, endocytosis, and related signaling by reducing Arpc2 levels.
• Assay development: Generate positive controls for high-content imaging or migration assays that are sensitive to actin dynamics.
• Omics integration: Combine siRNA knockdown with RNA-seq or proteomics to map downstream transcriptional or proteostatic effects.

Practical tips (general):

• Use RNase-free technique; thaw on ice, spin briefly, and keep concentrated stocks to minimize freeze–thaw.
• Start with a dose matrix (e.g., 1–50 nM final) and screen several transfection reagents; optimal conditions are cell-line specific.
• Employ multiple, independent siRNA duplexes to confirm on-target effects and reduce off-target concerns; a pre-designed set typically facilitates this strategy. For this item, the number of duplexes in the set is Not specified; refer to CoA/Spec Sheet.
• Validate knockdown by qPCR (24–48 h) and Western blot (48–96 h), accounting for target protein half-life.

Item-specific sequences, modifications, and recommended dosing: Not specified for this item; refer to CoA/Spec Sheet.

Reaction Conditions

This product is not used in traditional chemical reactions. The relevant “conditions” are those for efficient cellular delivery (general, literature guidance). Item-specific optimal parameters are not provided.

General transfection condition ranges:

• Cell lines: Adherent or suspension mammalian cells; optimize per cell type.
• Dosing: Start with 5–25 nM final siRNA; extend 1–50 nM for optimization. Higher doses may increase off-target effects (literature).
• Complexation: Mix siRNA with lipid/polymer transfection reagent in serum-free medium; incubate 10–30 min to form complexes.
• Exposure time: 4–24 h before media change, depending on reagent tolerability.
• Readouts: mRNA reduction typically detectable at 24–48 h; protein reduction 48–96 h depending on turnover.
• Controls: Include non-targeting siRNA, positive knockdown control, and mock transfection.

Environmental parameters (general):

• Temperature/CO2: Standard mammalian culture (37 °C, 5% CO2) unless working with specialized lines.
• Medium: Antibiotic-free during transfection; restore antibiotics after complex removal if required.

Item-specific guidance on reagent brand, ratios, sequences, and knockdown efficiency: Not specified for this item; refer to CoA/Spec Sheet.

Safety and Handling

Safety overview for an siRNA research reagent. Specific GHS details are not provided for this item; always consult the SDS for authoritative information.

• GHS/Classification: Not specified for this item; refer to SDS.
• Signal word / H-statements / Pictograms: Not specified for this item; refer to SDS.

General laboratory precautions (best practice):

• Handle using aseptic, RNase-free technique. Wear standard PPE: lab coat, nitrile gloves, safety glasses.
• Avoid generating aerosols; work in a clean area to prevent RNase contamination. Consider RNaseZap or equivalent decontamination of work surfaces and tools (general guidance).
• Incompatibilities (general): Avoid strong acids/bases and nucleases. Do not expose to prolonged elevated temperatures; protect from repeated freeze–thaw cycles.
• First aid (general): If contact with eyes/skin occurs, rinse with water. If inhaled (aerosol), move to fresh air. If ingested, rinse mouth. Seek medical attention if symptoms persist. Defer to SDS.
• Spill response: Wipe with absorbent material pre-wetted with suitable buffer or water; dispose of per institutional biosafety procedures.
• Waste: Dispose as laboratory biochemical waste according to local regulations; avoid drain disposal unless permitted.

Special risks (general for nucleic acids):

• Low inherent chemical hazard, but biological contamination risk (RNases) can compromise experimental integrity.
• Some formulations may contain salts or preservatives; for this item, formulation components are Not specified; consult CoA/SDS.

Solvent Selection

As a nucleic acid duplex, this product is designed for aqueous dissolution rather than organic solvents.

General solvent guidance for siRNA (literature):

• Primary solvents: RNase-free water or 1× TE (10 mM Tris–HCl, 1 mM EDTA, pH 7.5–8.0).
• Avoid: Protic organic solvents and strong bases/acids that can degrade RNA. Short exposures to ethanol/isopropanol occur during precipitation steps but are not used for stock solutions.
• Additives: For challenging cell types, siRNA is diluted into serum-free, antibiotic-free transfection medium just prior to complexation with a transfection reagent.

Polarity/compatibility (general):

• siRNA is highly polar and anionic, requiring aqueous buffers with monovalent salts (e.g., 10–150 mM Na+, K+) for stability and proper duplex behavior.

When to choose water vs TE (general):

• Use RNase-free water if you plan to complex immediately with cationic lipids/polymers; divalent chelation is unnecessary.
• Use TE for long-term frozen stocks; EDTA chelates trace Mg2+ that can catalyze RNA hydrolysis.

Item-specific diluent recommendations, concentration targets, and excipients: Not specified for this item; refer to CoA/Spec Sheet.

Storage and Reconstitution

Item-specific facts:

• Storage condition: Store at −20 °C (provided product data).
• Shipping: Ice chest + ice pads (provided product data).
• Research use: For research use only (provided product data).

General guidance for siRNA (literature; verify against CoA/Spec Sheet):

• Upon receipt: Inspect packaging temperature; centrifuge briefly to collect material. Maintain RNase-free conditions.
• Reconstitution: Dissolve in RNase-free water or 1× TE (pH 7.5–8.0) to a convenient stock (e.g., 20–100 µM). The exact nmol amount and target concentration for this item are Not specified; refer to CoA/Spec Sheet.
• Aliquoting: Prepare single-use aliquots to avoid repeated freeze–thaw. Store aliquots at −20 °C (short/medium term) or −80 °C (long term), protected from light if dye-labeled (no labeling is specified for this item).
• Handling: Thaw on ice, mix gently (no vortexing of dilute solutions to minimize shearing/foam), and keep on ice during setup.
• Shelf life: Follow CoA/SDS for stability and expiry. Absent item-specific data, reconstituted siRNA is commonly stable for several months at −80 °C and weeks at −20 °C when RNase-free (literature).
• Avoid: RNase contamination, alkaline pH, divalent cations, and multiple freeze–thaw cycles.

For definitive instructions (amount per vial, buffer composition if pre-formulated, and stability claims), consult the CoA/Spec Sheet for SKU A1466799.

Structure and Identity

Brief: Arpc2 Mouse Pre-designed siRNA Set A is an RNA interference reagent designed to transiently knock down the mouse Arpc2 gene (Actin-related protein 2/3 complex subunit 2). Item-specific identifiers such as sequence and oligo chemistry are not provided in the listing.

• SKU: A1466799
• Product type: Pre-designed siRNA set targeting mouse Arpc2
• CAS: Not specified for this item; refer to CoA/Spec Sheet.
• Molecular formula: Not specified for this item; refer to CoA/Spec Sheet.
• Molecular weight: Not specified for this item; refer to CoA/Spec Sheet.
• InChIKey / SMILES: Not applicable to polynucleotide mixtures; Not specified for this item; refer to CoA/Spec Sheet.

Structural features (general, literature):

• siRNA typically comprises a double-stranded 19–21 nt RNA duplex with 2-nt 3′ overhangs; antisense strand guides RISC to the target mRNA.
• Backbone: phosphodiester RNA; some products may include stabilizing modifications (e.g., 2′-O-methyl, phosphorothioate) at select positions, but specific modifications for this item are Not specified; refer to CoA/Spec Sheet.
• 2D description: Two antiparallel RNA strands (sense/antisense) forming an A-form helix; terminal 3′ overhangs (if present) reduce off-target loading (literature).

Identity elements specific to this item:

• Target gene: Arpc2 (mouse) inferred from product name; exact transcript isoform(s) and targeting sequences are Not specified for this item; refer to CoA/Spec Sheet.
• Set composition (number of duplexes, individual nmol amounts): Not specified for this item; refer to CoA/Spec Sheet.

Synthetic Utility

Not a small-molecule building block. This product is a functional siRNA duplex set for gene knockdown and is not typically used as a reagent in chemical synthesis.

Nevertheless, from a methodological perspective (general):

• siRNA serves as a biological tool to interrogate synthetic biology circuits and validate targets prior to small-molecule inhibitor development.
• In chemical biology workflows, siRNA knockdown can help deconvolute target engagement for probe compounds and guide structure–activity relationship (SAR) campaigns by confirming on-target phenotypes.

Any claims about reactive handles, conjugation chemistry, or linker functional groups for this item: Not specified for this item; refer to CoA/Spec Sheet.

Target Specificity

Only product-data-derived details can be asserted here.

• Target gene: Arpc2 (from product name)
• Species: Mouse (from product name)

Further specifics such as targeted transcript IDs/isoforms, exact siRNA sequences, off-target assessment, and in silico design criteria are Not specified for this item; refer to CoA/Spec Sheet.