GRADE & PURITYRecombinant?Recombinant — produced via recombinant expression for defined sequence and consistency. Use for reproducible, animal-free proteins of known origin.EnzymoPure™?EnzymoPure™ — Aladdin's line of high-quality enzymatic solutions. Use when enzyme purity and defined activity drive assay or process performance.1 mg/ml，＞ 1.0 x 10^10 relative light units (RLU)/ml Specific activity: 1.9 x 10^11 RLU/mg purified protein
EnzymoPure™, Recombinant, 1 mg/ml，＞ 1.0 x 10^10 relative light units (RLU)/ml Specific activity: 1.9 x 10^11 RLU/mg purified protein Recombinant,EnzymoPure™ for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 2 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
Product introduction
Firefly luciferase is an enzyme that catalyzes production of light from luciferin in the presence of Mg2+-ATP and oxygen. The reaction of this enzyme with luciferin, ATP, and O2 results in the emission of light. Luciferase activity can be inhibited by general anesthetics including isoflurane and ketamine/medetomidine thereby affecting the sensitivity of bioluminescence imaging.
Application
The reaction of this enzyme with luciferin, ATP, and O2 results in the emission of light. Luciferase can be used to detect trace amounts of ATP. Firefly luciferase is also one of the most commonly utilized reporter genes for the study of gene expression. The bioluminescent reaction catalyzed by luciferase is one of the most sensitive analytical tools for measuring gene expression. < or equal to one femtomole of ATP can be detected using 0.2 μg of luciferase.
Specifications
Product Name
Biotinylated Luciferase from E. coli, CAS No.61970-00-1
1.Wang Zhengwei, Lin Jiani, Tan Guisen, Pan Tingzheng, Zhang Hongtian, Liu Jiangang. (2025) miR-1270 suppresses glioblastoma development by transcriptional inhibition of nuclear factor IX. MOLECULAR BIOLOGY REPORTS, 52 (1):(1-10). [PMID:40815328][10.1007/s11033-025-10915-3]
2.Weiwei Liu, Qiuyue Ou, Meichang Feng, Yue Li, Linghan Huang, Xuan Peng, Xiaoqiang Zhong, Guoqiang Huang, Hengbo Wang, Jinlong Guo. (2025) Cold-Induced Promoter Methylation Attenuates ScMYB7-Mediated Repression of the CBF Pathway: A Proposed Mechanism for Sugarcane Cold Adaptation. PLANT CELL AND ENVIRONMENT, [PMID:40947544][10.1111/pce.70180]
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