Blood Sodium Content Assay Kit (Micro Method)

Cat. No.: B1521959

GRADE & PURITY BioReagent

Panoramica

Serum sodium plays an important role in maintaining normal extracellular fluid volume, osmotic pressure, and the acid-base balance of body fluids. This product is suitable for detecting serum samples. Its principle is based on the fact that sodium in the serum forms a precipitate with potassium pyroantimonate reagent in a weakly alkaline solution. The amount of precipitate is proportional to the sodium concentration. The sodium content in the serum can be determined by measuring the turbidity.

Specifications

Specifiche e purezza

BioReagent

Grado

BioReagent

Applicazione

Cell Metabolism

Tipo di campione

Siero

Metodo di rilevamento

Colorimetric Method

Detection instrument

Microplate reader,Visible Spectrophotometer

Detection wavelength

520nm

Stoccaggio e spedizione

Condizioni di conservazione di stoccaggio

Store at 2-8°C,Protected from light

Spedito in

Wet ice

Stabilità e conservazione

Store at 2-8℃ for 6 months. Store in the dark.

Application Protocols

Item-specific, validated protocols are not provided in the Product Data; consult the kit insert. The following is general guidance for a microplate colorimetric Na+ assay (literature/general):

• Materials:
  • Sodium standards (traceable), assay buffer/reagent mix, microplate, plate reader (vis range), Na+-free water, plastic pipette tips and tubes.
• Workflow:
  1. Equilibrate reagents to room temperature; protect light-sensitive reagents with foil.
  2. Prepare a standard curve (e.g., 0–200 mmol/L) by serial dilution using Na+-free water or matrix-matched diluent.
  3. Pipette standards, blanks, and appropriately diluted samples into wells (in duplicate or triplicate).
  4. Add the chromogenic reagent/buffer mix to each well in equal volume; mix gently without bubbles.
  5. Incubate for the recommended time until color develops (monitor kinetics to confirm plateau).
  6. Measure absorbance at the specified wavelength; use a reference wavelength if indicated.
  7. Generate the calibration curve; interpolate sample concentrations and apply dilution factors.
  8. Verify QC acceptance criteria (back-calculated standards within tolerance; consistent replicates).
• Notes:
  • Use fresh standards each run; avoid glass to minimize Na+ contamination.
  • For turbid or colored matrices, run matrix blanks and perform spike-recovery.

Exact reagent volumes, wavelengths, and timings for SKU B1521959 are not specified here.

Biological Roles

• Sodium ion (Na+) functions (literature/general):
  • Principal extracellular cation governing osmotic balance and extracellular fluid volume.
  • Critical for membrane potential, action potential propagation, and secondary active transport (e.g., Na+/glucose cotransport).
  • Influences enzyme activities and protein conformations via ionic strength and electrostatic screening.
  • Participates in pH regulation through Na+/H+ exchangers and bicarbonate transport.
• Experimental implications:
  • Monitoring Na+ in cell culture media can indicate evaporation, contamination, or formulation errors.
  • In biochemical assays, Na+ concentration affects protein stability, binding kinetics, and spectroscopic baselines.
  • Accurate Na+ quantification is essential when formulating buffers to maintain isotonicity in in vitro systems.
• Interferences and matrix effects (literature/general):
  • High K+, Ca2+, Mg2+, and ammonium can impact some Na+ colorimetric chemistries; masking agents or selective ligands are commonly used.
  • Hemolysis, lipemia, and high protein content can scatter light or bind dyes, altering apparent absorbance; sample clarification and appropriate blanks help mitigate.

Note: The above describes biological roles of Na+ in research contexts and does not imply clinical diagnostic use.

Buffer Applications

This product is an assay kit for Na+ quantification rather than a dedicated buffering agent.

• Relevance (literature/general):
  • The kit can be used to verify Na+ content in prepared buffers and saline solutions (e.g., PBS, HBSS) to ensure intended ionic strength in research workflows.
  • When preparing calibration standards or diluents, use Na+-free water and accurately weighed NaCl or traceable standards to avoid systematic bias.
• Practical tips:
  • Avoid glass volumetrics for standards when ultra-trace Na+ control is required; high-grade plasticware is preferred to minimize Na+ leaching.
  • Filter turbid buffer samples prior to measurement to reduce light scattering artifacts in colorimetric reads.

Item-specific buffer formulations or recipes: Not specified for this item; refer to CoA/Spec Sheet.

Green Alternatives

• Micro-method advantages (greener-by-design, literature/general):
  • Reduced reagent and sample volumes lower chemical consumption and waste generation.
  • Aqueous systems often avoid hazardous organic solvents.
  • Room-temperature operation in many colorimetric assays reduces energy usage compared with techniques requiring flame or plasma.

Comparison (general):

• Sodium determination options (literature/general):
  • Flame photometry: Sensitive but requires fuel gases and generates combustion emissions; higher energy and safety burden.
  • Ion-selective electrode (ISE): Minimal reagents but needs frequent calibration and can suffer from protein/fouling interferences.
  • Colorimetric micro assays (this kit type): Low volume, simple optics; chemical waste is typically low-to-moderate and largely aqueous.

Greener practice tips:

• Prepare only the reagent volume needed for the day.
• Consolidate washes and use multi-channel pipetting to minimize disposables.
• Segregate hazardous from non-hazardous waste to reduce disposal impact.
• Verify whether any reagent contains heavy metals or halogenated components; if present, collect separately (item-specific composition not specified).

Pharmaceutical Uses

This product is for research use only (Product Data) and is not intended for clinical diagnostic or therapeutic applications.

• Potential roles in non-clinical pharmaceutical R&D/QC (literature/general):
  • Quantification of Na+ in formulation development samples (e.g., saline-based excipient systems) to confirm target ionic strength.
  • Support in compatibility studies where Na+ concentration influences protein stability or excipient interactions.
  • In-process checks of rinse waters and buffer concentrates for manufacturing development labs (research stage).

Regulatory status, pharmacopeial alignment, and method validation parameters (accuracy/precision/robustness): Not specified for this item; users should develop/validate methods internally if needed.

Physical Properties

• Physical state: Multi-reagent kit (solutions and/or lyophilized solids). Exact physical forms: Not specified for this item; refer to CoA/Spec Sheet.
• Color/appearance of individual reagents: Not specified for this item; refer to CoA/Spec Sheet.
• Boiling point, melting point, density, refractive index, pKa, logP: Not applicable for a multi-component kit; not specified for this item.
• Solubility: Kit reagents are typically aqueous or water-miscible (literature/general). Any reconstitution solvent requirements should follow the kit insert.
• Optical properties: Micro-method sodium assays are commonly read in the visible range (for colorimetric chemistries), e.g., 540–660 nm depending on the chromophore (literature/general). The exact wavelength for this item is not specified; refer to the kit insert.
• Light sensitivity: Product should be protected from light per Product Data; keep reagents in amber vials or foil-wrap as applicable.

Quality and Grades

• Grade: BioReagent (Product Data). This typically indicates suitability for biological research applications where reagent background and impurities are controlled to minimize assay interference (general definition). It is not a clinical diagnostic grade and not intended for human or animal use.
• What BioReagent means in practice (general):
  • Low background signal and consistent reagent performance in common biochemical assays.
  • Controlled bioburden and particulates to reduce baseline drift and optical scattering.
  • Batch-to-batch consistency appropriate for research workflows.
• Item-specific specifications (e.g., lot acceptance criteria, linear range, LoD, interference screen, stabilizers): Not specified for this item; refer to CoA/Spec Sheet and kit insert.
• Documentation: For quantitative work, calibrate with the supplied or recommended Na+ standards from the same lot when possible to ensure traceability (general best practice).

Reaction and Applications

This kit supports quantitative measurement of sodium ion in biological or aqueous research samples using a micro-volume colorimetric/photometric format (general description).

• Application scope (research use only):
  • Determination of Na+ in cell culture supernatants, tissue homogenates, environmental water samples, or buffer formulations (literature/general).
  • High-throughput screening in microplates due to micro method format; reduced sample/reagent consumption.
• Chemical principle (literature/general):
  • Colorimetric complexation: A Na+-selective ligand (often crown-ether–derived or ionophore-based) forms a complex with Na+, producing a proportional absorbance change at a visible wavelength.
  • Alternatively, ion-exchange/indicator displacement strategies may be employed where Na+ competitively displaces a dye from a resin or carrier, altering absorbance.
• Practical tips:
  • Use Na+-free water and plasticware to avoid contamination.
  • Include a reagent blank and a full calibration curve with each run; matrix-match standards when feasible.
  • Minimize potassium and other cation interferences by following any provided conditioning steps or masking protocols in the kit instructions (literature/general).
• Not specified for this item: exact wavelength, linear range, LoD/LoQ, incubation times, and sample volumes; consult the kit insert/CoA.

Reaction Conditions

Item-specific operating parameters are not provided; consult the kit insert. The following reflects general conditions for micro-volume, colorimetric Na+ assays (literature/general):

• Format: 96-well plates or microcuvettes; pathlength ∼0.3–1.0 cm depending on platform and volume.
• Temperature: Ambient (20–25°C) unless otherwise specified by reagent kinetics.
• Wavelength: Visible region typical for the chosen chromophore (e.g., 540–660 nm). Exact λmax depends on the dye system.
• Reaction time: 5–30 minutes to reach stable color; verify linearity over time for your matrix.
• Calibration: ≥5 points spanning expected sample range; include reagent blank and matrix-matched QC(s).
• Sample volume: Micro-methods commonly use 2–50 µL sample with 50–250 µL reagent mix; optimize for signal-to-noise and plate optical path.
• Interference control: Follow any masking/conditioning steps to minimize K+, Ca2+, Mg2+ effects; centrifuge turbid samples.
• Data reduction: Use standard curve fit (linear or 4PL, as appropriate). Report as mmol/L or mEq/L; ensure proper dilution factor application.

Exact incubation times, reagent ratios, and stop steps for this SKU: Not specified for this item; refer to CoA/Spec Sheet.

Safety and Handling

• GHS classification, H-statements, pictograms, signal word: Not specified for this item; refer to SDS.
• General hazards (literature/general): Colorimetric sodium reagents and conditioning solutions may contain surfactants, mild acids/bases, or chelators. Avoid inhalation, ingestion, and skin/eye contact.
• PPE: Use lab coat, safety glasses, and appropriate chemical-resistant gloves. Work in a well-ventilated area or fume hood if aerosols or vapors may form.
• Incompatibilities: Keep acids and bases segregated; avoid contact between oxidizers and organics. Do not mix kit reagents with bleach unless the SDS indicates it is safe; some amines/chelators can generate hazardous byproducts with strong oxidants (general guidance).
• First aid (overview; defer to SDS):
  • Skin/eye contact: Rinse with water for ≥15 min; remove contaminated clothing; seek medical attention as needed.
  • Inhalation: Move to fresh air; seek medical attention if symptoms persist.
  • Ingestion: Rinse mouth; do not induce vomiting unless directed by medical personnel.
• Special risks: Protect from light (Product Data). For microplate/ cuvette work, prevent aerosol generation; treat all biological samples as potentially biohazardous and follow BSL-2 practices where applicable.
• Waste: Collect acidic/basic and dye-containing solutions as hazardous chemical waste (local regulations). Do not discharge into drains.

Solvent Selection

This is an aqueous, micro-volume analytical kit; conventional solvent selection is generally not applicable.

• Typical solvent environment (literature/general):
  • Assay buffer: Water-based buffer (e.g., Tris, HEPES, or proprietary) formulated to maintain ionic strength and pH for reliable Na+ complexation and color development.
  • Standards and samples: Prepared in water, saline, serum/plasma diluent, or matrix-matched solutions as directed in the kit insert.
• Practical notes:
  • Use freshly deionized water (≥18 MΩ·cm) to prepare dilutions to minimize background Na+.
  • Avoid glassware that can leach Na+; prefer high-quality plasticware for standards and blanks (literature/general).
  • If organic modifiers are present in a sample, confirm matrix compatibility and, if needed, perform appropriate dilutions or buffer exchange.

Storage and Reconstitution

• Storage conditions (Product Data): Store at 2–8°C, protected from light.
• Shipping (Product Data): Shipped on wet ice. Inspect upon arrival; if warmed, return to 2–8°C promptly.
• Stability: Item-specific shelf life and post-opening stability are not specified for this item; refer to CoA/Spec Sheet. In general, keep reagents tightly capped, minimize light exposure, and avoid repeated temperature cycling.
• Reconstitution:
  • Some kits include lyophilized components that require reconstitution with water or buffer. For this SKU, exact reconstitution volumes/solvents are not specified; refer to the kit insert.
  • Use Na+-free, high-resistivity water for any reconstitution or dilution to minimize background.
• Working solutions:
  • Prepare only the quantity needed for the day. Protect light-sensitive working reagents with amber tubes or foil.
  • Record preparation date and time; discard per guidance in the kit insert.
• Freeze–thaw guidance: Not specified. Unless directed otherwise, do not freeze reagents formulated for 2–8°C storage, as freezing may precipitate components or affect performance.
• General handling: Equilibrate cold reagents to room temperature before use; mix gently to redissolve any precipitates without foaming.
• Research use: For research use only (Product Data).

Structure and Identity

This product is a multi-component analytical kit for quantitative determination of sodium ions (Na+) in biological samples using a micro-volume format. It is not a single defined chemical entity.

• Item type: Assay Kit (colorimetric/photometric micro method; general description, literature)
• Target analyte: Sodium ion (Na+)
• Components: Typically include a chromogenic or complexing reagent, assay buffer, Na+ standards, and stop/conditioning solutions (general kit composition; not item-specific).
• Molecular formula / weight / SMILES / InChIKey: Not applicable to a kit. Not specified for this item; refer to CoA/Spec Sheet.
• Structural features: The chemistry commonly relies on Na+-selective complexation (e.g., crown-ether–based chromophores) or ion-exchange/color-forming couples that yield a measurable absorbance change upon binding Na+ (literature/general). 2D description is not applicable to a kit.

Synthetic Utility

Not a synthetic building block or reagent. This kit is designed for analytical quantification of Na+ in research samples. For information relevant to chemical synthesis, see Reaction & Applications and Reaction Conditions for analytical workflow guidance.

Target Specificity

• Analyte: Sodium ion (Na+).
• Intended selectivity: High preference for Na+ over common cations (K+, Ca2+, Mg2+, NH4+) using selective complexation or masking strategies (literature/general). Actual selectivity profile for this item is not specified; refer to kit documentation.
• Known potential interferents (literature/general):
  • Elevated K+ can compete in some ionophore systems; selectivity coefficients vary by chemistry.
  • High protein/turbidity can cause optical scatter; lipids may impart background absorbance.
  • Strong chelators (e.g., EDTA) in samples may sequester metal ions and perturb indicator behavior.
• Controls:
  • Use internal QCs and spike-recovery experiments to assess matrix effects.
  • Verify linearity and absence of significant bias across the expected Na+ range for your matrix.

No antibody/epitope/clone/isotype information applies; this is a small-molecule chemistry-based assay kit.