Aladdin Blue Latex Beads are produced through a proprietary deep-dyeing technique applied to polystyrene latex, resulting in vibrant color saturation. The dye is uniformly distributed within the microspheres, with minimal adsorption on the surface or release into the dispersion medium. This product is widely applicable in lateral flow assays and latex agglutination tests.
Product Name
| Catalog Number
| Surface Group
| Particle Size
| Concentration
|
Blue Latex Beads
| B1506731-A200nm
| Carboxyl (COOH)
| 200nm | 4% (40mg/mL) |
Blue Latex Beads
| B1506731-A300nm
| Carboxyl (COOH)
| 300nm
| 4% (40mg/mL) |
Blue Latex Beads
| B1506731-A400nm
| Carboxyl (COOH)
| 400nm
| 4% (40mg/mL) |
Blue Latex Beads
| B1506731-B200nm
| Streptavidin (SA)
| 200nm
| 1% (10mg/mL) |
Blue Latex Beads
| B1506731-B300nm
| Streptavidin (SA)
| 300nm
| 1% (10mg/mL) |
Blue Latex Beads
| B1506731-B400nm
| Streptavidin (SA)
| 400nm
| 1% (10mg/mL) |
Product Features
Proprietary deep-dyeing technology ensures deep, vibrant colors.
Dye is incorporated internally, resulting in a clean surface and a dispersion medium free of dye particles.
Highly uniform particle size with CV ≤ 3% and low batch-to-batch variation.
Instructions for Use
Example Antibody Conjugation Protocol (Using Carboxyl-functionalized Microspheres)
1. Recommended Buffers
Name
| Details
| Preparation Method
|
MES Buffer
| 50mM MES, pH 6.0
| 1.06g MES monohydrate (CAS: 145224-94-8) dissolved in 80mL purified water, adjust to pH 6.0 and adjust to 100mL.
|
sulfo-NHS Solution
| 10mg/mL in MES
| 100mg sulfo-NHS (CAS: 145224-94-8) dissolved in 10mL MES buffer.
|
EDC Solution
| 10mg/mL in MES
| 100mg EDC (CAS: 25952-53-8) dissolved in 10mL MES buffer.
|
Blocking Buffer
| 50mM HEPES, 1% BSA, pH 8.0
| 1.19g HEPES (CAS: 7365-45-9) dissolved in 80mL purified water, adjust to pH 8.0 and adjust to 100mL. Dissolve 1.0g BSA (CAS: 9048-46-8) in the buffer. |
2. Experimental Procedure
(1) Washing: Take 120 μL of Blue Latex Beads (4% solid content). Disperse by ultrasonication using an ultrasonic cell disruptor. Centrifuge (RCF > 15,000 × g) for 5 minutes and remove the supernatant. Wash once by centrifugation with MES Buffer.
(2) Activation: Add 300 μL of MES Buffer, pre-cooled on crushed ice. Add 40 μL of sulfo-NHS Solution, mix well. Then add 20 μL of EDC Solution, mix well, and react at room temperature for 20 minutes. Centrifuge (RCF > 15,000 × g) for 5 minutes, remove the supernatant, and wash once by centrifugation with MES Buffer.
(3) Antibody Conjugation: Add 480 μL of MES Buffer, disperse by ultrasonication. Add 160 μg of antibody, mix well, and react at room temperature for 4 hours. Centrifuge (RCF > 15,000 × g) for 5 minutes and remove the supernatant.
(4) Blocking: Add 480 μL of Blocking Buffer, disperse by ultrasonication, and react at room temperature for 1 hour. Centrifuge (RCF > 15,000 × g) for 5 minutes, remove the supernatant, and wash once by centrifugation with Blocking Buffer.
(5) Storage: Add 480 μL of Storage Buffer (same as Blocking Buffer; small amounts of stabilizers may be added), disperse by ultrasonication, and store at 2–8 °C.
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