Boc-Val-Pro-Arg-MCA hydrochloride - ≥98% , CAS No.70375-24-5

CAS: 70375-24-5 Cat. No.: B649765 Formula: C₃₁H₄₆ClN₇O₇ Peso molecolare: 664.19 PubChem CID: 16219060

GRADE & PURITY ≥98%

Storage

Store at -20°C,Desiccated

Shipped In

Ice chest + Ice pads

Panoramica

Boc-Val-Pro-Arg-MCA hydrochloride is a sensitive fluorogenic substrate for measuring trypsin-like serine proteases activity.

In Vitro

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs). Trypsin activity assay1. Add diluted Trypsin enzyme (100 μL) to 800 μL of 50 mM Tris–HCl buffer (pH 8.0). 2. The reaction is immediately initiated by the addition of 100 μL of 50 μM substrate Boc-Val-Pro-Arg-MCA. 3. Incubate at 55 °C for 10 min. 4. Add 1.5 mL of the stopping agent (methyl alcohol:n-butyl alcohol:distilled water = 35:30:35, v/v/v) to stop the reaction. 5. Measure the fluorescence intensity by a spectrofluorometer (excitation 380 nm, emission 450 nm). MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Form:Solid

Specifications

Specifiche e purezza

≥98%

Meccanismi biochimici e fisiologici

Boc-Val-Pro-Arg-MCA hydrochloride is a sensitive fluorogenic substrate for measuring trypsin-like serine proteases activity.

Condizioni di conservazione di stoccaggio

Store at -20°C,Desiccated

Spedito in

Ice chest + Ice pads

Questo prodotto richiede spedizione a catena fredda. I servizi di terra e altri servizi economici non sono disponibili.

Purezza

≥98%

Nomi e identificatori

Sorrisi canonici

CC1=CC(=O)OC2=C1C=CC(=C2)NC(=O)C(CCCN=C(N)N)NC(=O)C3CCCN3C(=O)C(C(C)C)NC(=O)OC(C)(C)C.Cl

Isomeri SMILES

CC1=CC(=O)OC2=C1C=CC(=C2)NC(=O)C(CCCN=C(N)N)NC(=O)C3CCCN3C(=O)C(C(C)C)NC(=O)OC(C)(C)C.Cl

PubChem CID

16219060

Peso molecolare

664.19