Brefeldin A Solution (1000 ×) - sterile-filtered,BioReagent,1000 ×

Cat. No.: B1508371

GRADE & PURITY Sterile-filtered, BioReagent, 1000 ×

Synonyms

BFA

Storage

Store at -20°C

Shipped In

Ice chest + Ice pads

Application

Flow Cytometry

Overview

Brefeldin A (BFA) is an intracellular protein transport inhibitor. Adding BFA during in vitro cell activation blocks protein transport to the Golgi Complex, causing accumulation in the Endoplasmic Reticulum, thereby enhancing the detection signal for intracellular cytokine staining. This method is particularly useful and effective for staining most cytokines in mouse and human cells. Optimal conditions, including the duration of treatment, are cell type and assay-dependent. Typically, BFA is added for the last few hours up to 4-24 hours prior to cell harvest. Researchers are advised to evaluate the performance of BFA, along with other transport inhibitors like monensin, in their specific assay system. Brefeldin A Solution is commonly supplied as a 1,000X concentrate and should be diluted to a 1X working concentration in cell culture medium.

Usage Protocol

Dilute the 1000X solution to 1X in the cell culture medium. 

Precautions

It is recommended that cells are cultured with BFA for ≤ 24 hours, as this can become toxic for cell viability.

Specifications

Synonyms

BFA

Specifications & Purity

sterile-filtered, BioReagent, 1000 ×

Stability And Storage

Store at -20℃ long term (12 months) or store at 4℃ short term (6 months).

Storage

Store at -20°C

Shipped In

Ice chest + Ice pads

This product requires cold chain shipping. Ground and other economy services are not available.

Grade

BioReagent, Sterile-filtered

Application

• Application: Dilution info
• Flow Cytometry:

  1/1000;