GRADE & PURITYBioReagent?BioReagent grade — tested suitable for life-science and molecular-biology use. Use for cell culture, assays, and biochemical work needing biological compatibility.Suitable for mass spectrometry (MS)?MS grade — low non-volatile and ionic residues compatible with mass spectrometry. Use to minimize background and ion suppression in MS workflows.≥99%(HPLC)
BioReagent,suitable for mass spectrometry (MS),≥99%(HPLC) Adatto alla spettrometria di massa (MS),BioReagent for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Panoramica
Protein denaturation and sample preparation for enzymatic digestion are the most critical experimental steps in peptide mapping, glycan analysis of biopharmaceutical antibodies, and proteomics research.
Native proteins exist in a three-dimensional structure. To expose digestion sites within the higher-order structure and enable efficient interaction with proteases, proteins must be fully denatured, reduced, alkylated, and subjected to necessary enzymatic reactions.
Traditional denaturants such as urea, guanidine hydrochloride, or SDS can cause certain chemical modifications to proteins after denaturation, such as carbamylation, leading to interference and errors in polypeptide identification.
The use of BT Surfactant avoids such modification issues.
Reconstitution:
Reconstitute in pH 8.0 buffer or ultrapure water.
Add 500 μL buffer to 5 mg BT Surfactant to obtain a 1% solution.
pH Range:
pH 7–9 for protein denaturation or enzymatic reactions.
BT Surfactant degrades and precipitates in solution at pH 2–4.
Recommended Usage:
Add 1–2% BT Surfactant to samples for protein denaturation.
Perform proteolytic digestion in 0.1% BT solution after denaturation.
Effect of BT Surfactant Concentration on Protease Activity:
BT concentration <2%: no effect on trypsin activity.
BT concentration <1%: no effect on Lys-C activity.
BT concentration <1%: no effect on rPNGase F glycosidase activity.
Effect of Temperature:
Incubation at 37 °C for 4 h: BT degradation peak <1% in MS.
Incubation at 95 °C for 10 min: BT degradation peak <5% in MS.
Specifications
Specifiche e purezza
BioReagent,suitable for mass spectrometry (MS),≥99%(HPLC)
Condizioni di conservazione di stoccaggio
Store at -20°C
Spedito in
Ice chest + Ice pads
Questo prodotto richiede spedizione a catena fredda. I servizi di terra e altri servizi economici non sono disponibili.
Grado
Adatto alla spettrometria di massa (MS), BioReagent
Purezza
≥99%(HPLC)
Documentazione
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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