≥98%(T) for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
Room temperature Ships Normal Check lot-specific COA for exact specifications.
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
Cited in 1 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
product description：
Calcein Blue is a cell-permeable dye that stains viable cells. Upon entering the cell, intracellular esterases cleave the acetoxymethyl (AM) ester group, yielding the membrane-impermeable Calcein Blue fluorescent dye. The staining applications of Calcein blue include tumor tissues, mineralized tissues, bone, amino acids, and many more.
application：
Calcein blue has been used fluorescent labeling of the mineralised sections of bone. Calcein blue AM ester hydrolyzed to calcein blue is suitable as a a viability stain, both microscopically, and in fluorescence activated cell sorting systems. It is also used as a merker of living cells like Schwann cells at neuromuscular junctions.
| Pubchem Sid | 504763817 |
|---|---|
| Pubchem Sid Url | https://pubchem.ncbi.nlm.nih.gov/substance/504763817 |
| Canonical Smiles | CC1=CC(=O)OC2=C1C=CC(=C2CN(CC(=O)O)CC(=O)O)O |
| IUPAC Name | 2-[carboxymethyl-[(7-hydroxy-4-methyl-2-oxochromen-8-yl)methyl]amino]acetic acid |
| InChIKey | RJPSHDMGSVVHFA-UHFFFAOYSA-N |
| INCHI | 1S/C15H15NO7/c1-8-4-14(22)23-15-9(8)2-3-11(17)10(15)5-16(6-12(18)19)7-13(20)21/h2-4,17H,5-7H2,1H3,(H,18,19)(H,20,21) |
| Isomeric SMILES | CC1=CC(=O)OC2=C1C=CC(=C2CN(CC(=O)O)CC(=O)O)O |
| Molecular Weight | 321.29 |
| Reaxy-Rn | 18100934 |
| Reaxys-RN_link_address | https://www.reaxys.com/reaxys/secured/hopinto.do?context=S&query=IDE.XRN=18100934&ln= |
Comprehensive hazard, handling, storage, and regulatory compliance document.
Download SDS →Lot-specific quality data. Enter your lot number to retrieve the exact COA.
Look up COA →Full quality attributes and acceptance criteria for this grade.
View spec sheet →Taxonomy Tree
| Kingdom | Organic compounds |
|---|---|
| Superclass | Phenylpropanoids and polyketides |
| Class | Coumarins and derivatives |
| Subclass | Hydroxycoumarins |
| Intermediate Tree Nodes | Not available |
| Direct Parent | 7-hydroxycoumarins |
| Alternative Parents | Alpha amino acids 1-benzopyrans Pyranones and derivatives Aralkylamines 1-hydroxy-2-unsubstituted benzenoids Dicarboxylic acids and derivatives Heteroaromatic compounds Trialkylamines Lactones Amino acids Oxacyclic compounds Carboxylic acids Organic oxides Hydrocarbon derivatives Carbonyl compounds |
| Molecular Framework | Aromatic heteropolycyclic compounds |
| Substituents | 7-hydroxycoumarin - Alpha-amino acid - Alpha-amino acid or derivatives - Benzopyran - 1-benzopyran - 1-hydroxy-2-unsubstituted benzenoid - Phenol - Pyranone - Aralkylamine - Benzenoid - Dicarboxylic acid or derivatives - Pyran - Heteroaromatic compound - Amino acid or derivatives - Amino acid - Lactone - Tertiary aliphatic amine - Tertiary amine - Oxacycle - Organoheterocyclic compound - Carboxylic acid - Carboxylic acid derivative - Organonitrogen compound - Carbonyl group - Organic oxide - Organooxygen compound - Organic nitrogen compound - Organic oxygen compound - Amine - Hydrocarbon derivative - Aromatic heteropolycyclic compound |
| Description | This compound belongs to the class of organic compounds known as 7-hydroxycoumarins. These are coumarins that contain one or more hydroxyl groups attached to the C7 position the coumarin skeleton. |
| External Descriptors | Not available |
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 F2206110 | Certificate of Analysis | Mar 18, 2026 | C153992 |
| F2206114 | Certificate of Analysis | Mar 18, 2026 | C153992 |
| F2206123 | Certificate of Analysis | Mar 18, 2026 | C153992 |
| F2206309 | Certificate of Analysis | Mar 18, 2026 | C153992 |
| I2122145 | Certificate of Analysis | Jul 10, 2025 | C153992 |
| I2122146 | Certificate of Analysis | Jul 10, 2025 | C153992 |
| I2122147 | Certificate of Analysis | Jul 10, 2025 | C153992 |
| Molecular Weight | 321.280 g/mol |
|---|---|
| XLogP3 | -1.800 |
| Hydrogen Bond Donor Count | 3 |
| Hydrogen Bond Acceptor Count | 8 |
| Rotatable Bond Count | 6 |
| Exact Mass | 321.085 Da |
| Monoisotopic Mass | 321.085 Da |
| Topological Polar Surface Area | 124.000 Ų |
| Heavy Atom Count | 23 |
| Formal Charge | 0 |
| Complexity | 514.000 |
| Isotope Atom Count | 0 |
| Defined Atom Stereocenter Count | 0 |
| Undefined Atom Stereocenter Count | 0 |
| Defined Bond Stereocenter Count | 0 |
| Undefined Bond Stereocenter Count | 0 |
| The total count of all stereochemical bonds | 0 |
| Covalently-Bonded Unit Count | 1 |
| 1. Xiuying Liu, Si Kang, Wen Wang, Lijie Zhu, Wei Zhang, Pingping Wang, Zaixi Shu, Yiwei Tang. (2024) Ratiometric fluorescent test strips based on CB-Ni2+@CDs probes for visual detection of histamine. Food Chemistry-X, [PMID:38883917] [10.1016/j.fochx.2024.101522] |
