Calretinin Mouse mAb - Anticorpo primario, specific to CALB2

Cat. No.: Ab092593

GRADE & PURITY ExactAb™, Validated, 1.0 mg/mL

Application

IHC

Host species

Mouse

Reactive species

Umano

Isotype

Mouse IgG1

Clone Number

UMAB306

Conjugation

Unconjugated

Panoramica

Purity

Immunogen affinity purified

Tested applications

Suitable for: WB , ICC/IF , IHC-P more details

Function

Calretinin is a calcium-binding protein which is abundant in auditory neurons.

Specifications

Product Name

Calretinin Mouse mAb - Anticorpo primario, specific to CALB2

Sinonimi

ATP-PFK | PFK-C | PFK-P | PFKF | 1200015H23Rik antibody | 6 phosphofructokinase type C antibody | 6 phosphofructokinase, platelet type antibody | 6-phosphofructokinase antibody | 6-phosphofructokinase type C antibody | 9330125N24Rik antibody | FLJ40226 an

Specifiche e purezza

ExactAb™, Validated, 1.0 mg/mL

Meccanismi biochimici e fisiologici

Calretinin is a calcium-binding protein which is abundant in auditory neurons.

Specie ospite

Mouse

Specificità

CALB2

Immunogeno

Recombinant protein of human Calretinin (AA 1-271).

Controllo positivo

IHC: Human appendix, adrenal gland, mesothelioma tissue.

Coniugazione

Unconjugated

Grado

ExactAb™, Validated

SDS-PAGE

150 kDa

Metodo di purificazione

Protein A/G purified

Concentrazione

1.0 mg/mL

Product Line

Prodotti biologici

Stoccaggio e spedizione

Forma

Liquid

Condizioni di conservazione di stoccaggio

Store at -20°C,Avoid repeated freezing and thawing

Spedito in

Ice chest + Ice pads

Stabilità e conservazione

Store at 2-8℃ short term (1 months). Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Obiettivi associati (umani)

CALB2 Tbio Calretinin (0 Activities)

Discover Target: CALB2

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Application Protocols

Item-specific tested applications and recommended dilutions

• Not specified for this item; refer to CoA/Spec Sheet.

General protocols (literature; to be optimized for this item)

• Western blot (WB): Prepare lysates with RIPA or mild lysis buffer + protease inhibitors. Denature in Laemmli buffer (with DTT or β‑ME), run SDS‑PAGE, transfer to PVDF. Block 5% milk/BSA. Incubate primary antibody overnight at 4°C at an empirically determined dilution (e.g., 1:500–1:5,000 typical range, not item-specific). Wash, incubate with species‑appropriate HRP secondary, develop by chemiluminescence.
• Immunohistochemistry (IHC‑FFPE): Deparaffinize/rehydrate, HIER (citrate pH ~6 or Tris‑EDTA pH ~9), quench endogenous peroxidase if using HRP, block, incubate with primary (optimize dilution), detect with polymer‑HRP and DAB or with fluorescent secondary. Counterstain and mount.
• Immunofluorescence (IF/ICC): Fix cells/tissue with 4% PFA, permeabilize (0.1–0.3% Triton X‑100), block with 3% BSA or serum, incubate with primary (optimize), wash, incubate with Alexa Fluor–type secondary, mount with anti‑fade medium.
• ELISA (indirect): Coat plates with recombinant calretinin (e.g., 1 µg/mL), block with 1% BSA, incubate with serial dilutions of the primary, detect with anti‑mouse HRP, TMB substrate, read at 450 nm.

Controls

• Include no-primary, isotype, and known positive/negative biological controls. Establish a dilution curve to identify the linear detection window for your assay.

Biological Roles

Target overview (literature; general biology of calretinin)

• Protein: Calretinin (gene CALB2) is a 29 kDa EF‑hand calcium-binding protein of the calmodulin superfamily. It contains multiple EF‑hand motifs that buffer and sense intracellular Ca2+.
• Expression: Prominent in specific neuronal subsets (e.g., cortical and hippocampal interneurons, cerebellar granule cells), sensory pathways, and in mesothelial tissues. It is also used to delineate certain neuronal lineages during development.
• Cellular functions: Modulates Ca2+ dynamics by fast buffering and may influence excitability, synaptic plasticity, and neurotransmitter release through its interactions with Ca2+ microdomains. Reported to interact with other signaling proteins and contribute to shaping Ca2+ transients (literature).
• Subcellular localization: Predominantly cytosolic with potential association to submembrane regions and organelles participating in Ca2+ handling.

Research relevance (no clinical claims)

• Neurobiology: Marker to classify interneuron subtypes alongside calbindin and parvalbumin; used in circuit mapping and developmental studies.
• Cell identity: In pathology research settings, calretinin immunoreactivity is leveraged to phenotype mesothelial-derived cells and to study differentiation programs. Any diagnostic or clinical use is outside scope; this product is for research use only.

Note for this item

• Species reactivity, clone, and epitope specificity for Ab092593 are not specified; consult the CoA/Spec Sheet for item-specific biological characterization.

Buffer Applications

Not typically applicable

• This product is an antibody reagent rather than a buffer component. It does not serve as a pH buffer system.

Practical note

• Successful use depends on compatible assay buffers (e.g., PBS/TBS-based diluents with appropriate blockers and detergents). Because the supplied buffer and additives for this item are not specified, validate compatibility with your chosen assay matrix and consider buffer exchange if required (e.g., azide-free for live cells).

Green Alternatives

Context

• Green chemistry considerations center on assay design and waste minimization rather than solvent replacement, since antibodies are aqueous biomolecules.

Strategies for greener antibody workflows (general; not item-specific)

• Reduce hazardous biocides: If sodium azide is present (not specified for this item), consider azide-free formulations for workflows where rapid use and refrigeration suffice. Evaluate microbial control needs versus toxicity and disposal burdens.
• Minimize single-use plastics: Employ reusable staining chambers, low-retention but washable reservoirs, and bulk dispensing to reduce tip/tube waste.
• Energy efficiency: Store at the warmest temperature compatible with stability (here, −20°C specified) and consolidate freezer space to reduce energy use. Use −80°C only if stability data demand it.
• Aqueous blocking and washing: Prefer water-based buffers with biodegradable detergents (e.g., polysorbates) over harsh solvents. Avoid methanol-based fixation unless required by the antigen.
• Waste handling: Segregate azide-containing solutions (if present) and use capture kits for heavy metals from HRP/DAB chromogens to prevent discharge.

Comparison (illustrative)

• Conventional: Azide-preserved antibody, DAB chromogen (hazardous), extensive single-use plastics.
• Greener: Azide-free short-term use, alkaline phosphatase with BCIP/NBT or fluorescent readouts as appropriate, and reduced plastic consumption. Tradeoffs include shelf-life and sensitivity depending on chosen detection chemistry.

Pharmaceutical Uses

Scope

• This reagent is designated “For research use only” and is not intended for diagnostic, therapeutic, or clinical applications.

General formulation roles for antibodies in manufacturing R&D (literature; not item-specific)

• Reference standards and controls: Monoclonal antibodies can serve as in-process controls or analytical reagents in bioprocess development (e.g., ligand binding assays), but such use requires item-specific qualification.
• Assay reagents: Capture/detection components in ELISA or biosensor platforms to support characterization of biologics.

Compliance note

• Pharmacopeial monographs do not generally cover research-grade antibodies like this item. For any GMP-adjacent use (e.g., lot release testing), qualification must include identity, purity, binding specificity, and stability studies specific to the intended method.

For this item

• No excipient role, formulation grade, or regulatory status is provided. Refer to the CoA/Spec Sheet if considering any regulated workflow in a research setting.

Physical Properties

Item-specific facts (from Product Data)

• Physical state/appearance: Not specified for this item; refer to CoA/Spec Sheet.
• Concentration, buffer composition, additives (e.g., BSA, glycerol, azide): Not specified for this item; refer to CoA/Spec Sheet.
• Molecular weight: Not specified for this item; refer to CoA/Spec Sheet.

General/literature values for antibodies (not specifications)

• Typical molecular mass: Intact IgG antibodies are ~150 kDa; Fab fragments ~50 kDa; Fc ~50 kDa (literature, varies with isotype and glycosylation).
• pI: Monoclonal IgGs often exhibit pI in the 6–9 range depending on sequence and glycoform (literature).
• Solubility: Readily soluble in aqueous buffers (e.g., PBS, TBS) at neutral pH; stability often enhanced with carrier proteins (e.g., BSA) and polyols (e.g., 10–50% glycerol) (literature, not item-specific).
• Stability considerations: Freeze–thaw cycles can induce aggregation and activity loss; storage at ≤−20°C or ≤−80°C in aliquots is standard to minimize denaturation (literature).

Notes for practitioners

• Without item-specific buffer and concentration, analytical confirmation (A280 with sequence-appropriate extinction coefficient, SDS-PAGE under reducing/non-reducing, SEC for aggregation) is recommended upon receipt.
• If viscosity or high concentration is encountered, gentle mixing at 4°C and low-shear handling help preserve activity.

Quality and Grades

Item-specific facts (from Product Data)

• Grade/Purity: ExactAb™, Validated.

What this typically signifies (general guidance; not a substitute for this item’s CoA)

• ExactAb™: Denotes a well-characterized monoclonal with defined identity and performance metrics across specified applications. Programs of this type often include sequence or clone traceability, lot-to-lot consistency controls, and orthogonal characterization (e.g., ELISA titer, Western blot signal-to-noise, and purity by SDS‑PAGE). Specific assays and acceptance criteria for this item are not specified—consult the CoA/Spec Sheet.
• Validated: Indicates the antibody has been performance-checked against its target under defined conditions. However, the validated application types and species reactivity are not specified for this item; verify against current documentation.

Recommended quality checks on receipt (best practices)

• Identity/Integrity: SDS‑PAGE (reducing/non-reducing), size‑exclusion chromatography for aggregation profile.
• Binding: Titration ELISA against recombinant calretinin; dot blot with serial dilutions to establish working range.
• Specificity: Western blot of positive vs negative control lysates (see Biological Roles for tissues/cell lines expressing calretinin). Include peptide competition only if using an epitope peptide matched to this clone (not specified here).
• Lot traceability: Record lot number, concentration, storage history, and create single-use aliquots to maintain performance.

Documentation

• For acceptance criteria, stabilizers (e.g., BSA, glycerol, azide), concentration, and validated applications, consult the lot-specific CoA/Spec Sheet.

Reaction and Applications

Applicability

• As a monoclonal antibody, this product is used in immunoassays rather than chemical synthesis. No manufacturer-validated applications are provided for this item.

General application contexts for anti-calretinin antibodies (literature; not item-specific claims)

• Immunohistochemistry (IHC): Detection of calretinin in neural tissues and mesothelial linings. Antigen retrieval protocols (citrate pH ~6 or EDTA pH ~9) are commonly explored; optimal conditions are clone-dependent.
• Immunofluorescence (IF/ICC): Visualization of calretinin-positive neuronal subpopulations in cultured neurons or tissue sections; permeabilization with 0.1–0.3% Triton X‑100 is typical for intracellular targets.
• Western blot (WB): Identification of calretinin (~29 kDa monomer; literature) in tissue or cell lysates; reducing SDS‑PAGE, followed by PVDF transfer.
• ELISA: Quantitative binding to recombinant calretinin or capture/detection in sandwich formats if paired antibodies recognizing non-overlapping epitopes are available (pairing not specified for this item).

Practical considerations

• Positive controls (literature): Brain regions rich in interneurons (e.g., cortex, hippocampus), dorsal root ganglia; calretinin-expressing cell lines when available. Negative controls: Isotype control, no-primary, or known calretinin‑low tissues.
• Titration: Establish a dilution curve to optimize signal-to-noise; start with broad ranges and refine per assay. Since no item-specific dilutions are provided, perform a small-scale matrix screen.

Reaction Conditions

Not applicable in the synthetic chemistry sense

• No chemical reaction conditions (solvents, catalysts, temperatures) apply. This product functions in immunoassays.

General assay conditions for anti-calretinin antibodies (literature; not item-specific)

• IHC: Formalin-fixed, paraffin-embedded (FFPE) sections often require heat-induced epitope retrieval (HIER) using citrate (pH ~6) or Tris‑EDTA (pH ~9). Blocking with serum or protein blockers reduces background.
• IF/ICC: Fix with 4% paraformaldehyde, permeabilize with 0.1–0.3% Triton X‑100 (for intracellular epitopes), block with 1–5% BSA or serum. Incubation 1–16 h at 2–8°C or 1 h at room temperature depending on affinity.
• WB: Load 10–30 µg total protein per lane; transfer to PVDF; block with 5% milk or BSA; primary incubation 1–16 h at 2–8°C.
• ELISA: Coat plates with 0.5–2 µg/mL recombinant antigen; block with casein/BSA; detect with species-appropriate HRP- or AP-conjugated secondary.

Note

• Because validated applications, working dilutions, and incubation parameters for Ab092593 are not specified, these conditions are starting points only and require empirical optimization.

Safety and Handling

Item-specific facts (from Product Data)

• Storage conditions: Store at −20°C. Avoid repeated freezing and thawing.
• Shipping: Ice chest + ice pads (cold-chain).
• GHS classification, signal word, pictograms, H‑statements: Not specified for this item; refer to SDS.

General safety guidance (literature/best practice; defer to SDS)

• PPE: Lab coat, gloves (nitrile), and safety glasses when handling vials and preparing dilutions.
• Handling: Work on ice or at 2–8°C to preserve activity. Avoid vigorous vortexing that can denature proteins; use gentle pipetting.
• Hygiene: Treat as a protein biological reagent; avoid ingestion and inhalation of aerosols. Wash hands after use.
• Spills: Absorb with paper towels or spill pads; decontaminate surfaces with 70% ethanol or appropriate lab disinfectant. Dispose of waste according to institutional biosafety procedures.
• Incompatibilities: Avoid strong oxidizers, extreme pH (<5 or >9) unless specified compatible, and proteases. Sodium azide (if present in buffer; not specified for this item) is toxic—collect azide-containing waste separately and avoid contact with metals in plumbing.
• First aid (overview): In case of skin contact, wash with water and soap. Eye contact—rinse cautiously with water for several minutes. If inhaled as an aerosol, move to fresh air. If ingested, rinse mouth. Seek medical advice if symptoms persist. Refer to SDS for authoritative guidance.
• Special risks: Repeated freeze–thaw cycles promote aggregation and loss of binding activity; aliquot upon first thaw.

Solvent Selection

Applicability

• Solvent selection in the classical synthetic sense is not typically applicable to monoclonal antibodies. These reagents are handled in aqueous buffers rather than organic solvents.

General guidance for antibody buffers (literature; not item-specific)

• Common storage/working buffers: PBS or TBS, pH 7.2–7.6, sometimes with additives such as 0.05–0.1% Tween‑20 (for assay buffers), 0.02–0.05% sodium azide (biocide), 0.1–1% BSA (carrier), and 10–50% glycerol for freeze protection. None of these are specified for this item.
• Avoid: Strongly denaturing solvents (e.g., high % DMSO, DMF, acetone), extremes of pH, and high ionic detergents unless the assay demands it.
• Compatibility: For IHC/IF, antibody diluents with protein blockers (serum, casein, BSA) and mild detergents can reduce background. For ELISA, carbonate-bicarbonate buffer (coating) and PBS/TBS (blocking/dilution) are common.

Practical tips

• If buffer composition is unknown (as here), pretest small aliquots for compatibility with your assay matrix. Dialysis or buffer exchange (spin columns) can adapt the antibody to special conditions (e.g., azide-free for live-cell staining).

Storage and Reconstitution

Item-specific facts (from Product Data)

• Storage conditions: Store at −20°C.
• Handling: Avoid repeated freezing and thawing.
• Shipping: Ice chest + ice pads.
• Reconstitution: Not specified for this item; refer to CoA/Spec Sheet.
• Formulation/buffer components and concentration: Not specified for this item; refer to CoA/Spec Sheet.

General guidance (literature; not item-specific)

• If received lyophilized: Briefly centrifuge to collect contents. Reconstitute with sterile deionized water or buffer (e.g., PBS) to the recommended concentration from the CoA. Allow to dissolve gently on ice; avoid foaming.
• If received in liquid: Upon first thaw, aliquot into low-protein-binding tubes at single-use volumes to prevent freeze–thaw.
• Storage temperatures: For short-term (≤1–2 weeks), 2–8°C is often acceptable. For medium to long-term, −20°C or −80°C is preferred depending on buffer (glycerol allows −20°C storage without freezing). Follow this item’s specified −20°C guidance.
• Protect from microbial contamination: If the formulation is azide-free (unknown here), practice sterile technique or add an appropriate preservative compatible with your application.
• Stability monitoring: Inspect for precipitation or turbidity. Test binding activity periodically by ELISA or a quick dot blot to confirm performance after storage.

Note

• Always refer to the lot-specific CoA/Spec Sheet for exact reconstitution volumes, diluents, stabilizers, and recommended storage durations for Ab092593.

Structure and Identity

Item-specific facts (from Product Data)

• Product: Calretinin Mouse mAb (monoclonal antibody)
• SKU: Ab092593
• Grade/Purity: ExactAb™, Validated
• CAS, CID, InChIKey, SMILES: Not applicable to protein antibodies; not specified for this item.
• Molecular formula and exact molecular weight: Not specified for this item; refer to CoA/Spec Sheet.

General description (literature)

• Molecule type: Mouse monoclonal IgG antibody directed against calretinin (target protein). IgG molecules are Y‑shaped glycoproteins composed of two heavy and two light chains linked by disulfide bonds, with variable (VH/VL) antigen-binding domains and constant (CH/CL) regions.
• Typical architecture: Two Fab arms (antigen binding) and one Fc region (effector). Disulfide bonds stabilize the hinge and intradomain β‑sandwich folds (immunoglobulin domains).
• Approximate size: Intact IgG antibodies are typically ~150 kDa (literature). Isotype and clone for this item are not specified.
• Target identity: Calretinin (CALB2) is an EF‑hand Ca2+‑binding protein of the calmodulin superfamily, predominantly expressed in subsets of neurons and certain mesothelial lineages (literature). Epitope recognized by this clone is not specified for this item.

2D structural features (descriptive)

• Antibody domains: Variable regions (framework + CDR loops) provide specificity to a conformational or linear epitope on calretinin; constant regions form the Fc. No small‑molecule structural identifiers are applicable.

Synthetic Utility

Not applicable

• As a biological affinity reagent, this monoclonal antibody is not used as a substrate, reagent, or catalyst in traditional synthetic organic chemistry.

Related laboratory utility

• Affinity capture: Antibodies can be immobilized on resins or magnetic beads for immunoprecipitation or pull‑down of calretinin and associated complexes from lysates (general literature guidance). For this item, coupling chemistry and binding capacity are not specified; optimization is required.

Target Specificity

Item-specific facts (from Product Data)

• Antigen/Target: Calretinin (CALB2)
• Antibody type: Mouse monoclonal antibody (mAb)
• Clone, isotype, epitope: Not specified for this item; refer to CoA/Spec Sheet.
• Species reactivity (tested): Not specified for this item; refer to CoA/Spec Sheet.
• Cross-reactivity data: Not specified for this item; refer to CoA/Spec Sheet.

General notes (literature)

• Calretinin is conserved across mammals; however, epitope accessibility can vary by fixation, species, and tissue processing. Clone- and epitope-specific validation is essential before critical experiments.