CAMTA2 Human Pre-designed siRNA Set A

Cat. No.: C1479745

Storage

Store at -20°C

Shipped In

Ice chest + Ice pads

Panoramica

CAMTA2 Human Pre-designed siRNA Set A contains three designed siRNAs for CAMTA2 gene (Human), as well as a negative control, a positive control, and a FAM-labeled negative control. Components CAMTA2 siRNA-1: 5 nmol (HPLC) CAMTA2 siRNA-2: 5 nmol (HPLC) CAMTA2 siRNA-3: 5 nmol (HPLC) siRNA Negative Control: 5 nmol (HPLC) FAM-labeled siRNA Negative Control: 5 nmol (HPLC) GAPDH siRNA Positive Control:5 nmol (HPLC)

Specifications

Condizioni di conservazione di stoccaggio

Store at -20°C

Spedito in

Ice chest + Ice pads

Questo prodotto richiede spedizione a catena fredda. I servizi di terra e altri servizi economici non sono disponibili.

Product Line

Prodotti biologici

Application Protocols

Item‑specific tested applications, recommended dilutions, and detailed protocols: Not specified for this item; refer to CoA/Spec Sheet and SDS.

Note: As a general practice (not item-specific), follow your transfection reagent’s instructions for complex formation, cell density, dosing, and incubation times when using siRNA in human cells. Validate knockdown by qPCR or immunoblot where possible and include appropriate controls.

Biological Roles

General literature context (not item-specific)

• Target: CAMTA2 encodes Calmodulin‑binding Transcription Activator 2, a nuclear transcription factor that integrates Ca2+/calmodulin signaling with gene expression programs.
• Domain architecture: contains a CG‑1 DNA‑binding domain, ankyrin repeats, IQ motifs for calmodulin interaction, and a transcriptional activation region.
• Cellular roles: participates in calcium‑responsive transcription, stress adaptation, and developmental gene regulation across tissues. CAMTA family members modulate expression of genes downstream of Ca2+ transients.
• Mechanistic note: Binding of Ca2+‑loaded calmodulin can modulate CAMTA2 activity, impacting its interaction with target promoters and co‑regulators.
• Experimental implications: RNAi‑mediated knockdown of CAMTA2 enables cause‑and‑effect studies of calcium‑regulated transcriptional networks, facilitating identification of direct versus indirect targets via time‑resolved transcriptomics and ChIP‑based assays.

Caveats

• Isoforms and redundancy within the CAMTA family may necessitate isoform‑specific assays or combinatorial knockdowns for unambiguous phenotypes.
• All statements above are general biological knowledge and not product‑specific. Refer to primary literature for organism-, cell type-, and context‑specific findings. No clinical or therapeutic claims are made.

Buffer Applications

This product is not a buffering agent. However, buffers are relevant for siRNA handling and storage.

General/literature guidance (not item-specific)

• Storage buffers: RNase‑free water or TE (10 mM Tris‑HCl, 1 mM EDTA, pH 7.5–8.0) are commonly used to stabilize siRNA stocks by chelating divalent cations that can promote nuclease activity.
• Working dilutions: prepare in serum‑free transfection medium immediately prior to delivery; after complex formation with the chosen transfection reagent, apply to cells and replace with complete medium per reagent instructions.
• Electrophoresis: non‑denaturing PAGE or agarose gels in TBE or TAE can assess duplex integrity and size; use RNase‑free conditions throughout.

Item-specific buffer details for this CAMTA2 siRNA set: Not specified for this item; refer to CoA/Spec Sheet.

Green Alternatives

Not applicable in the conventional solvent/chemical sense. This product is a nucleic‑acid reagent used in cell and molecular biology.

General sustainability considerations (not item-specific)

• Minimize plastic consumables by using multi‑use RNase‑free reservoirs and low‑waste pipetting strategies where contamination risk allows.
• Right‑size experiments to reduce reagent waste; prepare aliquots to extend shelf life and prevent discard due to freeze–thaw degradation.
• Dispose of nucleic acid materials following institutional biosafety and chemical waste guidelines to avoid environmental release of synthetic oligonucleotides.

Pharmaceutical Uses

Not applicable. This catalog item is designated for research use only and is not intended for human or veterinary use, diagnostics, or as a pharmaceutical ingredient.

General information (not item-specific)

• Some oligonucleotides can serve as excipients or actives in regulated contexts, but no such use is described or implied for this product. Any GMP status, pharmacopeial compliance, or clinical formulation relevance: Not specified for this item; refer to CoA/Spec Sheet.

Physical Properties

Item-specific properties

• Appearance: Not specified for this item; refer to CoA/Spec Sheet.
• Molecular weight / exact mass: Not specified for this item; refer to CoA/Spec Sheet.
• Solubility: Expected to be water‑soluble; item-specific solubility data not specified.
• pKa, logP, refractive index, BP/MP, density: Not applicable/Not specified for this polyanionic biopolymer; refer to CoA/Spec Sheet.

General/literature values (not item-specific)

• Typical siRNA length: ~19–27 nucleotides per strand; duplex mass commonly ~13–18 kDa depending on length and modifications.
• Solubility: readily soluble in RNase‑free water and low‑salt buffers (e.g., 10 mM Tris, 1 mM EDTA, pH ~7–8) at ≥10–100 µM.
• UV absorbance: strong at 260 nm; extinction coefficient depends on sequence and length (calculate by nearest‑neighbor method).

Practical notes

• Reconstitution concentrations commonly 10–100 µM in RNase‑free water or TE; aliquot to avoid freeze–thaw.
• Avoid divalent cations without chelator (EDTA) to minimize nuclease activity; handle with RNase‑free tools and gloves.

Quality and Grades

Item-specific quality

• Grade/purity: Not specified for this item; refer to CoA/Spec Sheet.
• Stabilizers/modifications: Not specified for this item; refer to CoA/Spec Sheet.

General context for siRNA quality (not item-specific)

• Common purity levels: desalted (crude), cartridge‑purified, HPLC‑purified (>85–95% by analytical HPLC/PAGE). Higher purity reduces off‑target effects and improves knockdown consistency.
• Identity & QC: sequence verification by mass spectrometry, analytical HPLC, or capillary electrophoresis; duplex annealing confirmation by non‑denaturing PAGE or melting profile.
• Endotoxin and bioburden: for cell culture applications, low endotoxin (<0.1 EU/µg) is desirable; RNase‑free status is critical.
• Annealed duplex vs. single strands: some products ship pre‑annealed; others provide separate sense/antisense strands for end‑user annealing.
• Formulation: lyophilized sodium salt, or solution in RNase‑free water/TE; presence of sodium counterions and residual salts impacts A260 quantitation.

Recommendation

• Consult the item’s CoA/Specification Sheet for actual purity, sequence, duplex state, and QC metrics specific to this CAMTA2 Human Pre‑designed siRNA Set A.

Reaction and Applications

This is a gene‑silencing reagent; chemical reaction applications (e.g., synthesis, catalysis) are not applicable.

General/literature applications for pre‑designed CAMTA2 siRNA (not item-specific)

• RNAi knockdown: transient suppression of CAMTA2 mRNA in human cells to study calcium/calmodulin‑responsive transcriptional programs and downstream gene networks.
• Pathway analysis: dissect roles of CAMTA2 in stress response and developmental transcription by coupling knockdown with RNA‑seq, qPCR, or reporter assays.
• Phenotypic screening: evaluate cell proliferation, differentiation, or signaling phenotypes upon CAMTA2 depletion; use multiple siRNA sequences and appropriate controls to confirm on‑target effects.
• Combination perturbations: co‑transfect with siRNA against pathway components to map genetic interactions.

Practical tips (general, not item-specific)

• Transfection optimization: titrate siRNA (e.g., 1–50 nM final) and reagent ratios; assess knockdown at 24–72 h by qPCR and at 48–96 h by Western blot if antibodies are available.
• Controls: include non‑targeting siRNA, positive control siRNA (against a housekeeping transcript), and mock transfection.
• Off‑target minimization: pool multiple low‑dose siRNAs or use independent sequences; validate with rescue experiments using siRNA‑resistant CAMTA2 cDNA.

Reaction Conditions

Not applicable to chemical synthesis.

General experimental conditions for siRNA use (literature; not item-specific)

• Cell transfection: many adherent human cell lines respond to 5–30 nM final siRNA; suspension lines may require optimization and electroporation.
• Timing: assess mRNA knockdown typically at 24–72 h; protein reduction may peak at 48–96 h depending on protein half‑life.
• Media: serum‑free medium during complex formation; reintroduce serum post‑transfection as recommended for the chosen reagent.
• Controls: include non‑targeting and positive‑control siRNA; perform dose–response to minimize cytotoxicity.

Item‑specific recommended conditions for this CAMTA2 set: Not specified for this item; refer to CoA/Spec Sheet and the transfection reagent manufacturer’s protocol.

Safety and Handling

Item-specific safety

• GHS classification, signal word, pictograms, H‑statements: Not specified for this item; refer to SDS.
• Research use only (per Product Data).

General safe handling for siRNA (not item-specific)

• PPE: lab coat, nitrile gloves, safety glasses. Use RNase‑free practices to prevent degradation (dedicated pipette tips, RNase‑free tubes, clean surfaces).
• Incompatibilities: RNases (ubiquitous on skin and benchtops), nucleases in non-sterile water; strong acids/bases and elevated temperatures can hydrolyze phosphodiester bonds.
• First aid (general): in case of contact with eyes/skin, rinse with water; if inhaled (lyophilized dust), move to fresh air; if ingested, rinse mouth. Seek medical attention as needed. Defer to SDS for authoritative guidance.
• Environmental: dispose of unused material and contaminated consumables as laboratory biological/chemical waste per institutional policy.
• Special risks: aerosolization of dry powders; use caution when opening lyophilized vials. Avoid repeated freeze–thaw that can lead to strand scission.

Defer to the official SDS for definitive hazard and response information for this catalog item.

Solvent Selection

This product is an siRNA reagent, not a small‑molecule solvent; conventional “solvent selection” is not applicable.

General guidance for reconstitution (literature; not item-specific)

• Use RNase‑free water or TE buffer (10 mM Tris‑HCl, 1 mM EDTA, pH 7.5–8.0) for stock solutions. TE can improve stability by chelating divalent cations.
• Avoid organic solvents (e.g., DMSO) unless validated for a specific delivery system; many transfection protocols require aqueous stocks.
• Typical working solutions: prepare 10–100 µM stocks; dilute into serum‑free transfection medium immediately before use per the chosen transfection reagent protocol.

For item‑specific solvent/buffer requirements, refer to the product’s CoA/Spec Sheet.

Storage and Reconstitution

Item-specific storage and shipping (from Product Data)

• Storage conditions: Store at −20°C.
• Shipping: Ice chest + ice pads.

Item-specific reconstitution details: Not specified for this item; refer to CoA/Spec Sheet.

General/literature guidance (not item-specific)

• Upon receipt: Centrifuge briefly to collect contents. Handle in an RNase‑free environment.
• Reconstitution: Dissolve in RNase‑free water or TE buffer (10 mM Tris‑HCl, 1 mM EDTA, pH 7.5–8.0) to 10–100 µM. Mix gently; avoid vigorous vortexing to limit shearing.
• Aliquoting: Prepare small aliquots to avoid repeated freeze–thaw cycles; store working stocks at −20°C (short/medium term). For extended storage, −80°C is commonly used (general practice).
• Stability: Protect from repeated freeze–thaw and nuclease contamination. Thaw on ice, keep cold during setup, and return unused aliquots to −20°C promptly.
• Quantitation: Measure A260 in RNase‑free buffer; calculate concentration using extinction coefficients derived from the sequence (if provided).

Structure and Identity

Item-specific facts (from Product Data)

• Product type: Pre-designed siRNA set targeting human CAMTA2 (SKU: C1479745)
• Target gene: CAMTA2 (Human)
• CAS: Not specified for this item; refer to CoA/Spec Sheet.
• Molecular formula: Not specified for this item; refer to CoA/Spec Sheet.
• Molecular weight: Not specified for this item; refer to CoA/Spec Sheet.
• SMILES / InChIKey: Not applicable to heterogeneous siRNA mixtures; not specified for this item.

General/literature description (not item-specific)

• siRNA are short double‑stranded RNAs typically ~19–21 bp with 2‑nt 3′ overhangs, phosphate backbone, ribose sugars, and standard or chemically modified bases.
• Structural features: two antiparallel strands (guide/passenger), 5′ phosphate (often), 3′ hydroxyl or modified termini; may include stabilizing modifications (e.g., 2′‑O‑Me, 2′‑F, phosphorothioates) depending on design.
• 2D description in words: linear polyanionic duplex composed of ribonucleotides linked via 3′→5′ phosphodiester bonds, forming an A‑form helical segment that is loaded into RISC to guide sequence‑specific mRNA cleavage.

Notes

• Specific sequences, length, strand orientation, and any modifications for this CAMTA2 set: Not specified for this item; refer to CoA/Spec Sheet.

Synthetic Utility

Not applicable. As a biologic oligonucleotide reagent, this product is not used as a building block or reagent in synthetic organic chemistry.

For experimental planning, please refer instead to the sections on Reaction & Applications, Biological Roles, and Storage & Reconstitution.

Target Specificity

Only product‑data facts

• Target: CAMTA2 (Calmodulin‑binding transcription activator 2)
• Species: Human

Additional details such as target transcript IDs, exon positioning, or sequence(s) of the siRNA set: Not specified for this item; refer to CoA/Spec Sheet.

Domande frequenti

How should this product be stored?

Store at ?20 °C. Freezer storage is required to maintain the specified shelf life.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.