CD16/CD32 Rat mAb (FITC) - Primary antibody, specific to CD16/CD32, Rat IgG2b

Cat. No.: Ab179682
AVAILABLE TO ORDER
GRADE & PURITY Validated ? Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated. ExactAb™ ? ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications. Azide Free ? Azide-free — without sodium azide preservative. Use in conjugations, cell work, or assays where azide is toxic or inhibitory. Ex:498nm ? Fluorescence excitation maximum at 498 nm — the wavelength of light that best excites this fluorophore. Match it to your instrument's laser or filter set for the strongest signal. Em:517nm ? Fluorescence emission maximum at 517 nm — the wavelength this fluorophore emits after excitation. Use it to pick the right detection filter and avoid channel cross-talk. 0.5 mg/mL
Application
Flow Cytometry
Host species
Rat
Reactive species
Mouse
Isotype
Rat IgG2b
Clone Number
2.4G2
Conjugation
FITC
 ·  off list, applied to all prices below.
Size
Status
Price
Qty
10μg
Ab179682-10μg
10
$59.90
25μg
Ab179682-25μg
3
$99.90
100μg
Ab179682-100μg
1
$299.90
Enter a quantity for the sizes you want to add.
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Why this grade

ExactAb™, Azide Free, Validated, Ex:498nm, Em:517nm, 0.5 mg/mL Azide Free,Em:517nm,Ex:498nm,ExactAb™,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.

🌡

Storage & shipping

Store at 2-8°C,Protected from light,Do not freeze Ships Wet ice,Do not freeze Check lot-specific COA for exact specifications.

📋

Quality documents

SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.

📚

Literature proof

Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.

Overview

Application:
Flow: ≤0.5μg/test
For flow cytometric staining, the suggested use of this reagent is ≤0.5 μg per million cells in 100 µL staining volume or 100 µL of whole blood.

Specifications

Product Name
CD16/CD32 Rat mAb (FITC) - Primary antibody, specific to CD16/CD32, Rat IgG2b
Synonyms
CD16 | CD16A | CD16-II | FCG3 | FCGR3 | FCGR3A | FCGRIII | FcGRIIIA | FCR-10 | FCRIII | FCRIIIA | IGFR3 | IMD20 | Low affinity immunoglobulin gamma Fc region receptor III-A | CD32/CD16 | Fc gamma RII/RIII | CD16a antibody | CD16b antibody | CD32 antibody
Specifications & Purity
ExactAb™, Azide Free, Validated, Ex:498nm, Em:517nm, 0.5 mg/mL
Host species
Rat
Specificity
CD16/CD32
Immunogen
Mouse BALB/c Macrophage J774
Positive Control
Flow: Murine splenocytes.
Conjugation
FITC
Excitation (nm)
Blue Laser(488nm)
Emission(nm)
517nm
Ex/Em(nm)
Ex:498nm;Em:517nm
Grade
Azide Free, Em:517nm, Ex:498nm, ExactAb™, Validated
Isotype
Rat IgG2b
Purification Method
Protein G purified
Concentration
0.5 mg/mL
Storage and Shipping
Shape
Liquid
Storage
Store at 2-8°C,Protected from light,Do not freeze
Shipped In
Wet ice,Do not freeze
Stability And Storage
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Images

CD16/CD32 Rat mAb (FITC) (Ab179682) - Flow Cytometry
Flow cytometry analysis of CD16/CD32 expression by mouse splenocytes cells (right panel) compared to an isotype control (left panel). Cells were stained with CD16/CD32 Rat mAb (FITC) (Ab179682) at 0.1μg/test for 1 hour at 4°C. Left panel - Rat IgG Isotype Control Antibody (FITC) (Ab180311).

CD16/CD32 Rat mAb (FITC) (Ab179682) - Flow Cytometry
Flow cytometry analysis of CD16/CD32 expression by mouse splenocytes cells (red) compared to an isotype control (blue). Cells were stained with CD16/CD32 Rat mAb (FITC) (Ab179682) at 0.1μg/test for 1 hour at 4°C. Blue - Rat IgG Isotype Control Antibody (FITC) (Ab180311). Black - Unlabelled control, cells without incubation with primary antibody.

Application
ApplicationDilution info
Flow Cytometry

≤0.5μg/test

For flow cytometric staining, the suggested use of this reagent is ≤0.5 μg per million cells in 100 µL staining volume.

Documentation

📋 Safety Data Sheet (SDS)

Comprehensive hazard, handling, storage, and regulatory compliance document.

Download SDS →

✅ Certificate of Analysis (COA)

Lot-specific quality data. Enter your lot number to retrieve the exact COA.

Look up COA →

📊 Datasheet

Quick-reference summary of product specifications and applications.

View datasheet →

🔬 Specification Sheet

Full quality attributes and acceptance criteria for this grade.

View spec sheet →

Advanced Data

Certificates(CoA,COO,BSE/TSE and Analysis Chart)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

4 results found

Lot NumberCertificate TypeDateItem
ZJ24F0303860Certificate of AnalysisJul 13, 2026 Ab179682
ZJ24F0303861Certificate of AnalysisJul 13, 2026 Ab179682
ZJ24F0303872Certificate of AnalysisJul 13, 2026 Ab179682
ZJ24R0300306Certificate of AnalysisMar 02, 2024 Ab179682
Documents & Articles
Solution Calculators
Reviews

Customer Reviews

Need help choosing the grade?

Our grade selection guide covers purity, stabilizer status, and application suitability for all variants in our catalog.

View Validated grade guide → View ExactAb™ grade guide → View Azide Free grade guide → View Ex:498nm grade guide → View Em:517nm grade guide →

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