Application:
Flow: ≤0.5μg/test
For flow cytometric staining, the suggested use of this reagent is ≤0.5 μg per million cells in 100 µL staining volume.
CD25 Rat mAb (Biotin) (Ab179014) - Flow Cytometry
BALB/c mouse splenocytes are stained with CD25 Rat mAb (Biotin) (Ab179014) and CD4 Rat mAb (APC) (Ab181112) (right), followed by Recombinant Streptavidin Protein (R-PE) (rp156150). BALB/c mouse splenocytes are stained with CD4 Rat mAb (APC) (Ab181112) and Rat IgG Isotype Control Antibody (Biotin) (Ab180310) (left).
CD25 Rat mAb (Biotin) (Ab179014) - Flow Cytometry
Con A-stimulated (1 day) BALB/c mouse splenocytes (right panel) stained with CD25 Rat mAb (Biotin) (Ab179014) (red) compared to an isotype control (blue). Blue - Rat IgG Isotype Control Antibody (Biotin) (Ab180310). Black - Unlabelled control, cells without incubation with primary antibody. Untreated BALB/c mouse splenocytes were used as control (left panel).
≤0.5μg/test
For flow cytometric staining, the suggested use of this reagent is ≤0.5 μg per million cells in 100 µL staining volume.
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Activity Type | Relation | Activity value | Units | Action Type | Journal | PubMed Id | doi | Assay Aladdin ID |
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Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Data | Oggetto |
|---|---|---|---|
 ZJ24F0404894 | Certificate of Analysis | Aug 03, 2026 | Ab179014 |
| ZJ24F0404895 | Certificate of Analysis | Aug 03, 2026 | Ab179014 |
| ZJ24F0404896 | Certificate of Analysis | Aug 03, 2026 | Ab179014 |
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