CD3 Rat mAb - Anticorpo primario, specific to CD3, Rat IgG2b

Cat. No.: Ab094424

GRADE & PURITY Carrier Free, ExactAb™, Azide Free, Validated, PBS Only, See COA

Application

Flow Cytometry

Host species

consiglio

Reactive species

Mouse

Isotype

Rat IgG2b

Clone Number

17A2

Conjugation

Unconjugated

Panoramica

Rat anti Mouse CD3 Antibody, Monoclonal (17A2), could be used for Flow Cytometry and so on.

Specifications

Product Name

CD3 Rat mAb - Anticorpo primario, specific to CD3, Rat IgG2b

Sinonimi

CD_antigen: CD3e | CD3 antigen, delta subunit | CD3 | CD3d antigen | CD3d antigen, delta polypeptide (TiT3 complex) | CD3d molecule, delta (CD3-TCR complex) | CD3-DELTA | CD3e antigen | CD3e antigen, epsilon polypeptide (TiT3 complex) | CD3e molecule, eps

Specifiche e purezza

Carrier Free,ExactAb™,Azide Free,Validated,PBS Only,See COA

Meccanismi biochimici e fisiologici

The CD3 complex mediates signal transduction.

Specie ospite

consiglio

Specificità

CD3

Immunogeno

γδTCR-positive T-T hybridoma D1

Controllo positivo

Flow Cytometry: Mouse splenocytes.

Coniugazione

Unconjugated

Grado

Azide Free, Carrier Free, ExactAb™, PBS Only, Validated

Isotipo

Rat IgG2b

Tipo di catena leggera

kappa

SDS-PAGE

150 kDa

Metodo di purificazione

Protein G purified

Concentrazione

See COA

Product Line

Prodotti biologici

Stoccaggio e spedizione

Forma

Liquid

Condizioni di conservazione di stoccaggio

Store at 2-8°C

Spedito in

Wet ice

Stabilità e conservazione

Shipped at 4°C. Store at 4°C long term (12 months). Upon receipt, it is recommended to aliquot.

Immagini

Flow Cytometry: CD3 Rat mAb (Ab094424)

CD3 Rat mAb (Ab094424) - Flow Cytometry
Flow Cytometry analysis of mouse splenocytes cells labelling CD3 with CD3 Rat mAb (Ab094424) conjugated with biotin (right panel) at 0.2 μg/mL for 1 hour at 4°C, compared with Rat IgG Isotype Control Antibody (Biotin) (Ab180310) - Isotype Control (left panel) and detected with SA-PE (rp156150).

Flow Cytometry: CD3 Rat mAb (Ab094424)

CD3 Rat mAb (Ab094424) - Flow Cytometry
Flow Cytometry analysis of mouse splenocytes cells labelling CD3 (red) with CD3 Rat mAb (Ab094424) conjugated with biotin at 0.2 μg/mL for 1 hour at 4°C, and detected with SA-PE (rp156150). Blue - Isotype control, Rat IgG Isotype Control Antibody (Biotin) (Ab180310). Black - Unlabelled control, cells without incubation with primary antibody.

Applicazione

Applicazione

Dilution info

Flow Cytometry

1μg/100w cells

Obiettivi associati (umani)

CD3G Tclin T-cell surface glycoprotein CD3 gamma chain (0 Activities)

Discover Target: CD3G

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Domande frequenti

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How should this product be stored?

Store at 2–8 °C. Refrigerated storage is required to maintain the specified shelf life.

How is this product shipped?

This product ships chilled on wet ice. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (CD3 Rat mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.