CD33 Mouse mAb (FITC) - Anticorpo primario, specific to CD33, Mouse IgG1

Cat. No.: Ab181122

QUALITÀ & PUREZZA Validated, Ex:498nm, Em:517nm, 5 μL/test

Application

Flow Cytometry

Host species

Mouse

Reactive species

Umano

Isotype

Mouse IgG1

Clone Number

HIM3-4

Conjugation

FITC

Panoramica

Application:
Flow: 5 μL/test
For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood.

Specifiche

Product Name

CD33 Mouse mAb (FITC) - Anticorpo primario, specific to CD33, Mouse IgG1

Sinonimi

CD33 antigen (gp67) | CD33 antigen | CD33 molecule | CD33 | FLJ00391 | gp67 | myeloid cell surface antigen CD33 | p67 | sialic acid binding Ig-like lectin 3 | Sialic acid-binding Ig-like lectin 3 | Siglec3 | Siglec-3 | SIGLEC3gp67 | CD 33 antibody | CD33

Specifiche e purezza

Validated, Ex:498nm, Em:517nm, 5 μL/test

Specie ospite

Mouse

Specificità

CD33

Immunogeno

NFMY-9s human cell line.

Controllo positivo

Flow: Human peripheral blood monocytes

Coniugazione

FITC

Eccitazione (nm)

Blue Laser(488nm)

Emissione (nm)

517nm

Ex/Em(nm)

Ex:498nm;Em:517nm

Grado

Em:517nm, Ex:498nm, Validated

Isotipo

Mouse IgG1

Tipo di catena leggera

kappa

Metodo di purificazione

Protein A purified

Concentrazione

5 μL/test

Product Line

Prodotti biologici

Stoccaggio e spedizione

Forma

Liquid

Condizioni di conservazione di stoccaggio

Store at 2-8°C,Protected from light,Do not freeze

Spedito in

Wet ice,Do not freeze

Stabilità e conservazione

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Immagini

Flow Cytometry: CD33 Mouse mAb (FITC) (Ab181122)

CD33 Mouse mAb (FITC) (Ab181122) - Flow Cytometry
Flow cytometry analysis of CD33 expression by Human peripheral blood (right panel) compared to an isotype control (left panel). Cells were stained with CD33 Mouse mAb (FITC) (Ab181122) at 5μL/test for 1 hour at 4°C. Left panel - Mouse IgG Isotype Control Antibody (FITC) (Ab180307).

Flow Cytometry: CD33 Mouse mAb (FITC) (Ab181122)

CD33 Mouse mAb (FITC) (Ab181122) - Flow Cytometry
Flow cytometry analysis of CD33 expression by Human peripheral blood monocytes (red) compared to an isotype control (blue). Cells were stained with CD33 Mouse mAb (FITC) (Ab181122) at 5μL/test for 1 hour at 4°C. Blue - Mouse IgG Isotype Control Antibody (FITC) (Ab180307). Black - Unlabelled control, cells without incubation with primary antibody.

Applicazione

Applicazione

Dilution info

Flow Cytometry

5μl/test

For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. 

Obiettivi associati (umani)

CD33 Tclin Myeloid cell surface antigen CD33 (0 Activities)

Discover Target: CD33

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Meccanismi d'azione

Domande frequenti

How should this product be stored?

Store at 2–8 °C, protected from light. Do not freeze. Freezing may cause irreversible damage to this material.

What does Validated mean?

Validated indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Ex:498nm indicate?

Ex:498nm is a product attribute used to filter and describe certain reagents in our catalog, rather than a standalone grade with its own specifications. It is most commonly used to identify products suitable for fluorescent labeling and detection.

How is this product shipped?

This product ships chilled on wet ice. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (CD33 Mouse mAb (FITC)); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.