MAb di topo CD4 (APC) - Anticorpo primario, specific to CD4, Mouse IgG2b

Cat. No.: Ab180133

GRADE & PURITY Validated, Ex:650nm, Em:660nm, 5 μL/test

Application

Flow Cytometry

Host species

Mouse

Reactive species

Umano

Isotype

Mouse IgG2b

Clone Number

OKT4

Conjugation

APC

Panoramica

Applicazione:
Flusso: 5 μL/test
Per la colorazione citometrica a flusso, l'uso suggerito di questo reagente è di 5 µL per milione di cellule in 100 µL di volume di colorazione o 5 µL per 100 µL di sangue intero.

Specifications

Product Name

MAb di topo CD4 (APC) - Anticorpo primario, specific to CD4, Mouse IgG2b

Sinonimi

CD_antigene: CD4 | Antigene CD4 (p55) | Antigene CD4 | Molecola CD4 | Recettore CD4 | CD4 | CD4mut | Antigene di superficie delle cellule T4/Leu-3 | Glicoproteina di superficie delle cellule T CD4 | Anticorpo CD 4 | Anticorpo CD4 (L3T4) | Anticorpo CD4 |

Specifiche e purezza

Convalidato, Ex:650nm, Em:660nm, 5 μL/test

Specie ospite

Mouse

Specificità

CD4

Immunogeno

Human peripheral T cells.

Controllo positivo

Flow: Human peripheral blood lymphocytes

Coniugazione

APC

Eccitazione (nm)

Red Laser(633nm)

Emissione (nm)

660nm

Ex/Em(nm)

Ex:650nm;Em:660nm

Grado

Em:660nm, Ex:650nm, Validated

Isotipo

Mouse IgG2b

Metodo di purificazione

Protein G purified

Concentrazione

5 μL/test

Product Line

Prodotti biologici

Stoccaggio e spedizione

Forma

Liquido

Condizioni di conservazione di stoccaggio

Conservare a 2-8°C, al riparo dalla luce, Vietato il congelamento

Spedito in

Ghiaccio umido

Stabilità e conservazione

La soluzione anticorpale deve essere conservata non diluita tra 2°C e 8°C, al riparo da un'esposizione prolungata alla luce. Non congelare.

Immagini

Flow Cytometry: CD4 Mouse mAb (APC) (Ab180133)

CD4 Mouse mAb (APC) (Ab180133) - Flow Cytometry
Flow cytometry analysis of CD4 expression by Human peripheral blood lymphocytes (right panel) compared to an isotype control (left panel). Cells were stained with CD4 Mouse mAb (APC) (Ab180133) at 5μL/test for 1 hour at 4°C. Left panel - Mouse IgG Isotype Control Antibody (APC) (Ab180309).

Flow Cytometry: CD4 Mouse mAb (APC) (Ab180133)

CD4 Mouse mAb (APC) (Ab180133) - Flow Cytometry
Flow cytometry analysis of CD4 expression by Human peripheral blood lymphocytes (red) compared to an isotype control (blue). Cells were stained with CD4 Mouse mAb (APC) (Ab180133) at 5μL/test for 1 hour at 4°C. Blue - Mouse IgG Isotype Control Antibody (APC) (Ab180309). Black - Unlabelled control, cells without incubation with primary antibody.

Applicazione

Applicazione

Dilution info

Flow Cytometry

5μl/test

For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. 

Obiettivi associati (umani)

CD4 Tclin T-cell surface glycoprotein CD4 (0 Activities)

Discover Target: CD4

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Meccanismi d'azione

Domande frequenti

How should this product be stored?

Store at 2–8 °C, protected from light. Do not freeze. Freezing may cause irreversible damage to this material.

What does Validated mean?

Validated indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Ex:650nm indicate?

Ex:650nm is a product attribute used to filter and describe certain reagents in our catalog, rather than a standalone grade with its own specifications. It is most commonly used to identify products suitable for fluorescent labeling and detection.

How is this product shipped?

This product ships chilled on wet ice. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (CD4 Mouse mAb (APC)); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.