CD43 Mouse mAb - Anticorpo primario, specific to CD43, Mouse IgG1

Cat. No.: Ab094843

GRADE & PURITY Carrier Free, Validated, ExactAb™, See COA

Application

Flow Cytometry

Host species

Mouse

Reactive species

Umano

Isotype

Mouse IgG1

Clone Number

HI161

Conjugation

Unconjugated

Panoramica

Mouse anti Human CD43 Antibody, Monoclonal (HI161), could be used for Flow and so on.
Application:
Flow:＜0.5 µg/100w cells
Protein Function
One of the major glycoproteins of thymocytes and T lymphocytes. Plays a role in the physicochemical properties of the T-cell surface and in lectin binding. Presents carbohydrate ligands to selectins. Has an extended rodlike structure that could protrude above the glycocalyx of the cell and allow multiple glycan chains to be accessible for binding. Is a counter receptor for SN/Siglec-1 (By similarity). During T-cell activation is actively removed from the T-cell-APC (antigen-presenting cell) contact site thus suggesting a negative regulatory role in adaptive immune response.

Specifications

Product Name

CD43 Mouse mAb - Anticorpo primario, specific to CD43, Mouse IgG1

Sinonimi

Galactoglycoprotein | GALGP | GPL115 | Leukocyte sialoglycoprotein | Sialophorin | LEU-22 | CD43 antigen | CD43 | CD43) | Galactoglycoprotein | GALGP | GPL115 | Leukocyte sialoglycoprotein | Leukosialin | LSN | Ly-48 | sialophorin (gpL115, leukosialin, CD

Specifiche e purezza

Carrier Free, ExactAb™, Validated, See COA

Specie ospite

Mouse

Specificità

CD43

Immunogeno

Human T lymphocytes

Controllo positivo

Flow: Human peripheral blood lymphocytes.

Coniugazione

Unconjugated

Grado

Carrier Free, ExactAb™, Validated

Isotipo

Mouse IgG1

Tipo di catena leggera

kappa

SDS-PAGE

150 kDa

Metodo di purificazione

Protein A purified

Concentrazione

See COA

Stoccaggio e spedizione

Forma

Liquid

Condizioni di conservazione di stoccaggio

Store at 2-8°C

Spedito in

Wet ice

Stabilità e conservazione

Shipped at 4°C. Store at 4°C long term (36 months). Upon receipt, it is recommended to aliquot.

Immagini

Flow Cytometry: CD43 Mouse mAb (Ab094843)

CD43 Mouse mAb (Ab094843) - Flow Cytometry
Flow Cytometry analysis of K562 cells labelling CD43 (red) with CD43 Mouse mAb (Ab094843) at 0.5 μg/mL for 1 hour at 4°C. Goat Anti-Mouse IgG (PE) (Ab179006) at a dilution of 1/1000 was used as the secondary antibody. Blue - Isotype control, mouse IgG (Ab170221). Black - Unlabelled control, cells without incubation with primary antibody.

Flow Cytometry: CD43 Mouse mAb (Ab094843)

CD43 Mouse mAb (Ab094843) - Flow Cytometry
Flow Cytometry analysis of U937 cells labelling CD43 (red) with CD43 Mouse mAb (Ab094843) at 0.05 μg/mL for 1 hour at 4°C. Goat Anti-Mouse IgG (PE) (Ab179006) at a dilution of 1/1000 was used as the secondary antibody. Blue - Isotype control, mouse IgG (Ab170221). Black - Unlabelled control, cells without incubation with primary antibody.

Flow Cytometry: CD43 Mouse mAb (Ab094843)

CD43 Mouse mAb (Ab094843) - Flow Cytometry
Flow Cytometry analysis of Ramos cells labelling CD43 (red) with CD43 Mouse mAb (Ab094843) at 0.5 μg/mL for 1 hour at 4°C. Goat Anti-Mouse IgG (PE) (Ab179006) at a dilution of 1/1000 was used as the secondary antibody. Blue - Isotype control, mouse IgG (Ab170221). Black - Unlabelled control, cells without incubation with primary antibody.

Applicazione

• Applicazione: Dilution info
• Flow:

  < 0.5µg/100w cells;

Obiettivi associati (umani)

SPN Tbio Leukosialin (0 Activities)

Discover Target: SPN

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Domande frequenti

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How should this product be stored?

Store at 2–8 °C. Refrigerated storage is required to maintain the specified shelf life.

How is this product shipped?

This product ships chilled on wet ice. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (CD43 Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.