CD44 Rat mAb (PE-Cy5) - Anticorpo primario, specific to CD44

Cat. No.: Ab190176

GRADE & PURITY ExactAb™, Validated, 0.5 mg/mL

Application

Flow Cytometry

Host species

consiglio

Reactive species

Mouse

Isotype

Rat IgG2b

Clone Number

IM7

Conjugation

PE-Cy5

Panoramica

Rat anti Mouse CD44 Antibody, Monoclonal (IM7), could be used for Flow Cytometry.

Specifications

Product Name

CD44 Rat mAb (PE-Cy5) - Anticorpo primario, specific to CD44

Sinonimi

Ly-24 | Pgp-1 | HERMES | CD44 antigen | CD44 molecule (Indian blood group) | CD44 | CD44R | CDw44 | cell surface glycoprotein CD44 | chondroitin sulfate proteoglycan 8 | CSPG8 | ECMR-III | epican | Extracellular matrix receptor III | GP90 lymphocyte homin

Specifiche e purezza

ExactAb™, Validated, 0.5 mg/mL

Meccanismi biochimici e fisiologici

Receptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA

Specie ospite

consiglio

Specificità

CD44

Immunogeno

Dexamethasone-induced myeloid leukemia M1 cells

Controllo positivo

Flow Cytometry: C57BL/6 mouse splenocytes

Coniugazione

PE-Cy5

Grado

ExactAb™, Validated

Tipo di catena leggera

kappa

Metodo di purificazione

Protein G purified

Concentrazione

0.5 mg/mL

Product Line

Prodotti biologici

Stoccaggio e spedizione

Condizioni di conservazione di stoccaggio

Store at 2-8°C,Protected from light,Do not freeze

Spedito in

Wet ice,Do not freeze

Stabilità e conservazione

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Immagini

Flow Cytometry: CD44 Rat mAb (PE-Cy5) (Ab190176)

CD44 Rat mAb (PE-Cy5) (Ab190176) - Flow Cytometry
Flow cytometry analysis of CD44 expression by C57BL/6 mouse splenocytes (right panel) compared to an isotype control (left panel). Cells were stained with CD44 Rat mAb (PE-Cy5) (Ab190176) at 0.04μg/test for 1 hour at 4°C. Left panel - Rat IgG (PE-Cy5) (Ab190072).

Flow Cytometry: CD44 Rat mAb (PE-Cy5) (Ab190176)

CD44 Rat mAb (PE-Cy5) (Ab190176) - Flow Cytometry
Flow cytometry analysis of CD44 expression by C57BL/6 mouse splenocytes (red) compared to an isotype control (blue). Cells were stained with CD44 Rat mAb (PE-Cy5) (Ab190176) at 0.04μg/test for 1 hour at 4°C. Blue - Rat IgG (PE-Cy5) (Ab190072). Black - Unlabelled control, cells without incubation with primary antibody.

Applicazione

Applicazione

Dilution info

Flow Cytometry

≤0.3μg/test

For flow cytometric staining, the suggested use of this reagent is ≤0.3 μg per million cells in 100 µL staining volume.

Obiettivi associati (umani)

CD44 Tbio CD44 antigen (0 Activities)

Discover Target: CD44

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Domande frequenti

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Validated mean?

Validated indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

How is this product shipped?

This product ships chilled on wet ice. Unpack on arrival and transfer it to the storage condition stated above.

How should this product be stored?

Store at 2–8 °C, protected from light. Do not freeze. Freezing may cause irreversible damage to this material.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (CD44 Rat mAb (PE-Cy5)); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.