CD45 Mouse mAb - Primary antibody, specific to Human CD45

Cat. No.: Ab230687
AVAILABLE TO ORDER
GRADE & PURITY Carrier Free ? Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays. Validated ? Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated. ExactAb™ ? ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications. Azide Free ? Azide-free — without sodium azide preservative. Use in conjugations, cell work, or assays where azide is toxic or inhibitory. High Performance ? High-performance grade with optimized purity and performance characteristics. Use for sensitive analyses where ordinary grades fall short. PBS Only ? PBS-only formulation — supplied in phosphate-buffered saline with no other additives. Use when you need a clean PBS buffer free of stabilizers/carriers. ≥95%(SDS-PAGE) 1.0 mg/mL
Application
ELISA, Flow Cytometry, WB
Host species
Mouse
Reactive species
Human
Isotype
Mouse IgG1
Clone Number
B01/5F1
Conjugation
Unconjugated
Size
USA
Germany (EU)*
Price
Qty
10μl
Ab230687-10μl
Made to order · 8–12 wks
$49.90
50μl
Ab230687-50μl
Made to order · 8–12 wks
$129.90
100μl
Ab230687-100μl
Made to order · 8–12 wks
$199.90
1ml
Ab230687-1ml
Made to order · 8–12 wks
$999.90
Enter a quantity for the sizes you want to add.
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Why this grade

Carrier Free,ExactAb™,Azide Free,Validated,High Performance,PBS Only,≥95%(SDS-PAGE),1.0 mg/mL Azide Free,Carrier Free,ExactAb™,High Performance,PBS Only,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.

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Storage & shipping

Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.

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Quality documents

SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.

📚

Literature proof

Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.

Overview

Mouse anti Human CD45 Antibody, clone B01/5F1, could be used for WB, Flow Cytometry, ELISA and so on.

Specifications

Product Name
CD45 Mouse mAb - Primary antibody, specific to Human CD45
Synonyms
B220 antibody | CD45 antibody | CD45 antigen antibody | CD45R antibody | EC 3.1.3.48 antibody | GP180 antibody | L-CA antibody | LCA antibody | Leukocyte common antigen antibody | Loc antibody | Ly-5 antibody | LY5 antibody | Ly5, homolog of antibody | Ly
Specifications & Purity
Carrier Free, ExactAb™, Azide Free, Validated, High Performance, PBS Only, ≥95%(SDS-PAGE), 1.0 mg/mL
Biochemical and Physiological Mechanisms
Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affec
Host species
Mouse
Specificity
Human CD45
Immunogen
Recombinant protein corresponding to human CD45
Positive Control
WB: Jurkat whole cell lysate Flow Cytometry: Jurkat cells
Conjugation
Unconjugated
Grade
Azide Free, Carrier Free, ExactAb™, High Performance, PBS Only, Validated
Light Chain Type
kappa
SDS-PAGE
150.0 KDa
Purification Method
Protein A purified
Concentration
1.0 mg/mL
Storage and Shipping
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 2-8℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Images

CD45 Mouse mAb (Ab230687) - Western Blot
All lanes: CD45 Mouse mAb (Ab230687) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 148 kDa
Observed band size: 202 kDa
Exposure time: 60.0 s

CD45 Mouse mAb (Ab230687) - Flow Cytometry ​
Overlay histogram showing Jurkat cells stained with CD45 Mouse mAb (Ab230687) (Red) at 1.25 μg/mL for 1 hour at 4°C. Cells were fixed and permeabilized with 100% methanol. The Goat Anti-Mouse IgG H&L (PE) (Ab179006) was used as the secondary antibody. Unlabeled sample (Black) was used as a negative control. Mouse IgG1-kappa, anti-E8L (Ab213557) was used as an isotype control (Blue).​

Application
ApplicationDilution info
WB

1/1000 - 1/2000

Flow Cytometry

1/200 - 1/6250

Documentation

📋 Safety Data Sheet (SDS)

Comprehensive hazard, handling, storage, and regulatory compliance document.

Download SDS →

✅ Certificate of Analysis (COA)

Lot-specific quality data. Enter your lot number to retrieve the exact COA.

Look up COA →

📊 Datasheet

Quick-reference summary of product specifications and applications.

View datasheet →

🔬 Specification Sheet

Full quality attributes and acceptance criteria for this grade.

View spec sheet →

Advanced Data

Associated Targets(Human)
PTPRC Tchem Receptor-type tyrosine-protein phosphatase C (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)
Certificates(CoA,COO,BSE/TSE and Analysis Chart)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:
Solution Calculators
Reviews

Customer Reviews

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