CD47 mAb di topo (biotina) - Anticorpo primario, specific to CD47, Mouse IgG1

Cat. No.: Ab185151

GRADE & PURITY ExactAb™, Validated, 0.5 mg/mL

Application

Flow Cytometry

Host species

Mouse

Reactive species

Umano

Isotype

Mouse IgG1

Clone Number

CC2C6

Conjugation

Biotin

Panoramica

Applicazione:
Flusso: ≤1,0 μg/test
Per la colorazione citometrica a flusso, l'uso suggerito di questo reagente è ≤1,0 μg per milione di cellule in 100 µL di volume di colorazione o 100 µL di sangue intero

Specifications

Product Name

CD47 mAb di topo (biotina) - Anticorpo primario, specific to CD47, Mouse IgG1

Sinonimi

antigene identificato dal monoclonale 1D8 | Proteina determinante della superficie antigenica OA3 | Antigene CD47 (antigene Rh-correlato, trasduttore di segnale associato all'integrina) | Antigene CD47 | Glicoproteina CD47 | Molecola CD47 | CD47 | IAP | P

Specifiche e purezza

ExactAb™, convalidato, 0,5 mg/mL

Specie ospite

Mouse

Specificità

CD47

Immunogeno

CCRF-CEM T-cell line.

Controllo positivo

Flow: MCF-7

Coniugazione

Biotin

Grado

ExactAb™, Validated

Isotipo

Mouse IgG1

Tipo di catena leggera

kappa

Metodo di purificazione

Protein G purified

Concentrazione

0.5 mg/mL

Product Line

Prodotti biologici

Stoccaggio e spedizione

Condizioni di conservazione di stoccaggio

Conservare a 2-8°C, vietato congelare

Spedito in

Ghiaccio umido

Stabilità e conservazione

La soluzione anticorpale deve essere conservata non diluita tra 2°C e 8°C, al riparo da un'esposizione prolungata alla luce. Non congelare.

Immagini

Flow Cytometry: CD47 Mouse mAb (Biotin) (Ab185151)

CD47 Mouse mAb (Biotin) (Ab185151) - Flow Cytometry
Flow cytometry analysis of CD47 expression by MCF-7 cells (red) compared to an isotype control (blue). Cells were stained with CD47 Mouse mAb (Biotin) (Ab185151) at 0.1μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark. Blue - Mouse IgG Isotype Control Antibody (Biotin) (Ab180306). Black - Unlabelled control, cells without incubation with primary antibody.

Applicazione

Applicazione

Dilution info

Flow Cytometry

≤1.0μg/test

For flow cytometric staining, the suggested use of this reagent is ≤1.0 μg per million cells in 100 µL staining volume. 

Obiettivi associati (umani)

CD47 Tchem Leukocyte surface antigen CD47 (0 Activities)

Discover Target: CD47

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Meccanismi d'azione

Domande frequenti

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Validated mean?

Validated indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

How should this product be stored?

Store at 2–8 °C. Do not freeze. Freezing may cause irreversible damage to this material.

How is this product shipped?

This product ships chilled on wet ice. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (CD47 Mouse mAb (Biotin)); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.