CD47 Rat mAb (PE) - Anticorpo primario, specific to CD47, Rat IgG2a

Cat. No.: Ab190284

GRADE & PURITY Validated, ExactAb™, Ex:565nm, Em:575nm, 0.5 mg/mL

Application

Flow Cytometry

Host species

consiglio

Reactive species

Mouse

Isotype

Rat IgG2a

Clone Number

MIAP301

Conjugation

PE

Panoramica

Rat anti Mouse CD47 Antibody, Monoclonal (MIAP301), could be used for Flow, in vivo CD47 blockade, in vitro CD47 blockade, IF, ICC and so on.

Specifications

Product Name

CD47 Rat mAb (PE) - Anticorpo primario, specific to CD47, Rat IgG2a

Sinonimi

Itgp | 9130415E20Rik | B430305P08Rik | antigen identified by monoclonal 1D8 | Antigenic surface determinant protein OA3 | CD47 antigen (Rh-related antigen, integrin-associated signal transducer) | CD47 antigen | CD47 glycoprotein | CD47 molecule | CD47 |

Specifiche e purezza

ExactAb™, Validated, Ex:565nm, Em:575nm, 0.5 mg/mL

Meccanismi biochimici e fisiologici

Has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. Plays an important role in memory formation and synaptic plasticity in the hippocampus (By similarity). Receptor for SIRPA, bind

Specie ospite

consiglio

Specificità

CD47

Immunogeno

Intact CD47 purified from placenta

Controllo positivo

Flow: C57BL/6 mouse splenocytes.

Coniugazione

PE

Eccitazione (nm)

Blue Laser(488nm), Green Laser(532nm), Yellow-Green Laser(561nm)

Emissione (nm)

575nm

Ex/Em(nm)

Ex:565nm;Em:575nm

Grado

Em:575nm, Ex:565nm, ExactAb™, Validated

Isotipo

Rat IgG2a

Tipo di catena leggera

kappa

Metodo di purificazione

Protein G purified

Concentrazione

0.5 mg/mL

Stoccaggio e spedizione

Condizioni di conservazione di stoccaggio

Store at 2-8°C,Protected from light,Do not freeze

Spedito in

Wet ice,Do not freeze

Stabilità e conservazione

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Immagini

Flow Cytometry: CD47 Rat mAb (PE) (Ab190284)

CD47 Rat mAb (PE) (Ab190284) - Flow Cytometry
Flow cytometry analysis of CD47 expression by C57BL/6 mouse splenocytes (red) compared to an isotype control (blue). Cells were stained with CD47 Rat mAb (PE) (Ab190284) at 0.05μg/test for 1 hour at 4°C. Blue - Rat IgG Isotype Control Antibody (PE) (Ab180312). Black - Unlabelled control, cells without incubation with primary antibody.

Applicazione

• Applicazione: Dilution info
• Flow Cytometry:

  ≤0.3 μg/test, the suggested use of this reagent is ≤0.3 μg per million cells in 100 µL staining volume ;

Obiettivi associati (umani)

CD47 Tchem Leukocyte surface antigen CD47 (0 Activities)

Discover Target: CD47

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Meccanismi d'azione