CD70 mAb di ratto - Anticorpo primario, specific to CD70, Rat IgG2b

Cat. No.: Ab095232

QUALITÀ & PUREZZA Carrier Free, Azide Free, Validated, PBS Only, ≥95%(SDS-PAGE), See COA

Application

Animal Model, Blocking, Flow Cytometry, Functional Assay

Host species

consiglio

Reactive species

Mouse

Isotype

Rat IgG2b

Clone Number

FR70

Conjugation

Unconjugated

Panoramica

L'anticorpo anti CD70 di topo, monoclonale (FR70), può essere utilizzato per il flusso, il blocco CD70 in vivo, il blocco CD70 in vitro e così via.
Applicazione:
Flusso: utilizzare una concentrazione dipendente dal dosaggio.
blocco CD70 in vivo: utilizzare a una concentrazione dipendente dal dosaggio.
blocco CD70 in vitro: utilizzare a una concentrazione dipendente dal dosaggio.
Funzione della proteina
Citochina che si lega al CD27. Svolge un ruolo nell'attivazione delle cellule T. Induce la proliferazione delle cellule T costimolate e aumenta la generazione di cellule T citolitiche

Specifiche

Product Name

CD70 mAb di ratto - Anticorpo primario, specific to CD70, Rat IgG2b

Sinonimi

Cd27l | Tnfsf7 | Tnlg8a | ligando CD27 | CD27-L | CD27LCD27 ligando | CD27LG | molecola CD70 | CD70 | antigene Ki-24 | antigene di superficie CD70 | TNFSF7 | TNFSF7CD70 antigene | fattore di necrosi tumorale (ligando) superfamiglia, membro 7 | fattore di

Specifiche e purezza

Privo di carrier, privo di azide, convalidato, ≥95% (SDS-PAGE), Vedere COA

Specie ospite

consiglio

Specificità

CD70

Immunogeno

BALB/c mouse B lymphoma A20.2J

Controllo positivo

IHC-Fr: Human tonsil, and human kidney tissue. Flow Cyt: Raji cells.

Coniugazione

Unconjugated

Grado

Azide Free, Carrier Free, PBS Only, Validated

Isotipo

Rat IgG2b

Tipo di catena leggera

kappa

SDS-PAGE

150 kDa

Metodo di purificazione

Protein G purified

Concentrazione

See COA

Product Line

Prodotti biologici

Stoccaggio e spedizione

Forma

Liquido

Condizioni di conservazione di stoccaggio

Conservare a 2-8°C, vietato congelare

Spedito in

Ghiaccio umido

Immagini

Flow Cytometry: CD70 Rat mAb (Ab095232)

CD70 Rat mAb (Ab095232) - Flow Cytometry
Flow Cytometry analysis of 10 μg/mL LPS stimulated 3 days mouse splenocytes cells labelling CD70 with CD70 Rat mAb (Ab095232) conjugated with biotin (right panel) at 0.5 μg/mL for 1 hour at 4°C, compared with Rat IgG Isotype Control Antibody (Biotin) (Ab180310) (left panel) and detected with SA-PE (rp156150).

Flow Cytometry: CD70 Rat mAb (Ab095232)

CD70 Rat mAb (Ab095232) - Flow Cytometry
Flow Cytometry analysis of mouse splenocytes cells labelling CD70 (red) with CD70 Rat mAb (Ab095232) conjugated with biotin at 0.5 μg/mL for 1 hour at 4°C, and detected with SA-PE (rp156150). Blue - Rat IgG Isotype Control Antibody (Biotin) (Ab180310). Black - Unlabelled control, cells without incubation with primary antibody.

Applicazione

Applicazione

Dilution info

Flow Cytometry

Use at an assay dependent concentration.

in vivo CD70 blockade

Use at an assay dependent concentration.

in vitro CD70 blockade

Use at an assay dependent concentration.

Obiettivi associati (umani)

CD70 Tbio CD70 antigen (0 Activities)

Discover Target: CD70

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Meccanismi d'azione

Domande frequenti

What is the purity of this product, and how is it determined?

This product is supplied at ≥95% purity, determined by SDS-PAGE. Lot-specific values are stated on the Certificate of Analysis.

What does Azide Free mean?

Azide Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How is this product shipped?

This product ships chilled on wet ice. Unpack on arrival and transfer it to the storage condition stated above.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How should this product be stored?

Store at 2–8 °C. Do not freeze. Freezing may cause irreversible damage to this material.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (CD70 Rat mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.