CD86 Rat mAb (AF700) - Anticorpo primario, specific to CD86

Cat. No.: Ab215325

GRADE & PURITY Validated, ExactAb™, 0.5 mg/mL

Application

Flow Cytometry

Host species

consiglio

Reactive species

Mouse

Isotype

Rat IgG2a

Clone Number

GL1

Conjugation

AF700

Panoramica

Rat anti Mouse CD86 Antibody, Monoclonal (GL1), could be used for Flow Cytometry.

Specifications

Product Name

CD86 Rat mAb (AF700) - Anticorpo primario, specific to CD86

Sinonimi

Activation B7-2 antigen | B70 | B7-2 antigen | B72 | B7-2 | B-lymphocyte activation antigen B7-2 | BU63 | CD28 antigen ligand 2 | CD28LG2B7-2 antigen) | CD86 antigen | CD86 molecule | CD86 | CTLA-4 counter-receptor B7.2 | FUN-1 | LAB72 | MGC34413 | T-lymp

Specifiche e purezza

ExactAb™, Validated, 0.5 mg/mL

Meccanismi biochimici e fisiologici

Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as d

Specie ospite

consiglio

Specificità

CD86

Immunogeno

LPS-activated CBA/Ca mouse splenic B cells

Controllo positivo

Flow: C57BL/6 mouse splenocytes

Coniugazione

AF700

Eccitazione (nm)

Red Laser(633nm)

Emissione (nm)

719nm

Ex/Em(nm)

Ex:696nm;Em:719nm

Grado

ExactAb™, Validated

Tipo di catena leggera

kappa

Metodo di purificazione

Protein G purified

Concentrazione

0.5 mg/mL

Stoccaggio e spedizione

Condizioni di conservazione di stoccaggio

Store at 2-8°C,Protected from light,Do not freeze

Spedito in

Wet ice,Do not freeze

Stabilità e conservazione

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Immagini

Flow Cytometry: CD86 Rat mAb (AF700) (Ab215325)

CD86 Rat mAb (AF700) (Ab215325) - Flow Cytometry
LPS-stimulated (3 days) C57BL/6 mouse splenocytes (right panel) stained with CD86 Rat mAb (AF700) (Ab215325) (red) compared to an isotype control (blue). Blue - Rat IgG (AF700) (Ab190242). Black - Unlabelled control, cells without incubation with primary antibody. Untreated C57BL/6 mouse splenocytes were used as control (left panel).

Flow Cytometry: CD86 Rat mAb (AF700) (Ab215325)

CD86 Rat mAb (AF700) (Ab215325) - Flow Cytometry
LPS-stimulated (3 days) C57BL/6 mouse splenocytes (right panel) stained with CD86 Rat mAb (AF700) (Ab215325) (right panel) at 0.3μg/test for 1 hour at 4°C. Left panel - Rat IgG (AF700) (Ab190242).

Applicazione

• Applicazione: Dilution info
• Flow Cytometry:

  ≤1.0μg/ test

  For flow cytometric staining, the suggested use of this reagent is ≤1.0 μg per million cells in 100 µL staining volume.;

Obiettivi associati (umani)

CD86 Tclin T-lymphocyte activation antigen CD86 (0 Activities)

Discover Target: CD86

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)