Cell Freezing Medium (with Serum) - sterile-filtered, BioReagent, endotoxin tested, ready-to-use, for cell culture

Cat. No.: C1491281

GRADE & PURITY Sterile-filtered, BioReagent, Endotoxin tested, Ready-to-use, for Cell culture

Synonyms

Cell Cryopreservation Solution

Storage

Store at -20°C

Shipped In

Ice chest + Ice pads

Application

Cell Culture

Panoramica

This product is a pre-sterilized, ready-to-use cryopreservation solution designed for the low-temperature freezing of cultured cells. After harvesting the cells to be preserved, simply add an appropriate amount of this solution and mix thoroughly—no additional preparation is required. It is highly convenient and suitable for cryopreserving most types of mammalian cells.

Features

• High Quality: Ensures cell resuscitation rates of over 90%.

• Ease of Use: Stable storage at 2–8°C; no freezing required—ready to use directly.

Instructions for Use

Cell Freezing Procedure

1. Harvest adherent or suspension cells in the logarithmic growth phase and perform cell counting.

2. Transfer the cell suspension containing the desired number of cells into a centrifuge tube. Centrifuge to pellet the cells (recommended conditions: 1,000–2,000 rpm, 3–5 min). Carefully remove the supernatant.

3. Add an appropriate amount of cryopreservation solution to the tube and adjust the cell concentration to 1–5×10⁶ cells/mL. Mix gently to prepare a uniform cell suspension.

4. Dispense the cell suspension into clearly labeled cryovials.

5. Place the cryovials in a device capable of controlled cooling (e.g., a programmed freezing container) and transfer to a -80°C freezer, ensuring a cooling rate of approximately 1°C/min. The cooling rate should generally not exceed 0.5°C/min.

6. For long-term storage in liquid nitrogen, transfer cryovials to a liquid nitrogen tank after overnight storage at -80°C.

Cell Thawing Procedure

1. Remove frozen cryovials and immediately thaw in a 37°C water bath with gentle agitation.

2. Once completely thawed, promptly transfer the cell mixture to a centrifuge tube containing 5 mL of cell culture medium. Centrifuge to pellet cells (recommended conditions: 1,000–2,000 rpm, 3–5 min).

3. Carefully aspirate the supernatant. Resuspend the cell pellet in an appropriate amount of fresh culture medium with gentle mixing. Transfer the cell suspension to a pre-prepared culture vessel.

4. After microscopic examination, proceed with routine cell culture according to specific research requirements.

Precautions 

• Ensure the cryopreservation solution is completely thawed and gently mixed before use.

• For long-term cell storage, transfer cryovials to a liquid nitrogen tank.

• When cryopreserving stem cells or primary cells, it is recommended to perform a preliminary test using this product for at least one week to verify performance before formal cryopreservation.

• To minimize the risk of cross-contamination, aliquot the product for repeated use.

• This solution contains DMSO. Wear a lab coat and disposable gloves for safety.

• For research use only.

Specifications

Sinonimi

Cell Cryopreservation Solution

Specifiche e purezza

sterile-filtered, BioReagent, endotoxin tested, ready-to-use, for cell culture

Stabilità e conservazione

Store at 2-8℃ short term (3 months). Store at -20℃ long term (12 months).

Condizioni di conservazione di stoccaggio

Store at -20°C

Spedito in

Ice chest + Ice pads

Questo prodotto richiede spedizione a catena fredda. I servizi di terra e altri servizi economici non sono disponibili.

Grado

BioReagent, Filtrato asetticamente, per la coltura cellulare, Pronto all’uso, Testato per le endotossine

Domande frequenti

What does sterile-filtered mean?

sterile-filtered indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

What does BioReagent mean?

BioReagent indicates that the product has been processed and tested for biological and biochemical applications. Specifications may include endotoxin limits, microbial controls, sterility, low DNase/RNase/protease activity, and verified biological activity where relevant.

How should this product be stored?

Store at ?20 °C. Freezer storage is required to maintain the specified shelf life.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.