Chicken Thyroid Stimulating Hormone (TSH) ELISA Kit

Cat. No.: EJ1515345

GRADE & PURITY BioReagent

Panoramica

This kit employs the competitive enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with Chicken TSH antigen. The sample, standards, a biotin-labeled antibody, and an HRP enzyme conjugate are then added sequentially to the wells. The procedure includes incubation and wash steps between each addition. TMB substrate is used for color development. In the presence of the peroxidase (HRP) enzyme, TMB is catalyzed to produce a blue color, which is then converted into a final stable yellow color upon the addition of an acid stop solution. The intensity of the resulting yellow color is inversely proportional to the concentration of Chicken TSH in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader, and the concentration of the target analyte in the sample is calculated based on the standard curve.

Specifications

Specifiche e purezza

BioReagent

Grado

BioReagent

Applicazione

ELISA

Tipo di campione

Plasma, Siero

Campo di rilevamento

0.312μIU/mL~20μIU/mL

Specie rilevata Oggetto

Chicken

Specificità

Detecting Chicken TSH in samples, with no significant cross-reactivity to other homologs.

Detection instrument

Microplate reader

Detection wavelength

450nm

Repeatability

The intra- and inter-assay coefficients of variation (CV) are both <10%.

Detection duration

3.5 hours, not including equilibration and sample preparation time.

Stoccaggio e spedizione

Condizioni di conservazione di stoccaggio

Store at 2-8°C

Spedito in

Wet ice

Stabilità e conservazione

Store at 2-8℃ for 6 months.

Application Protocols

Tested applications, recommended sample types, dilutions, incubation times, and plate maps are not specified for this item; refer to the kit insert for authoritative instructions.

Note: Only use the protocol supplied with the kit to ensure validated performance. Do not substitute reagents or alter steps unless validated in your laboratory.

Biological Roles

TSH (thyrotropin) is a pituitary glycoprotein hormone that regulates thyroid function. The kit targets chicken TSH for research quantification. The following describes TSH biology in general (literature):

• Structure and biosynthesis

  • Heterodimeric glycoprotein composed of a common α-subunit (shared with LH/FSH) and a hormone-specific β-subunit conferring receptor specificity. Both subunits are glycosylated, influencing secretion, stability, and receptor interaction.

• Mechanism of action

  • Binds the TSH receptor (TSHR), a GPCR on thyroid follicular cells, activating Gs–adenylate cyclase, elevating cAMP, and promoting iodide uptake, thyroglobulin processing, and thyroid hormone biosynthesis.

• Regulation

  • Hypothalamic TRH stimulates TSH synthesis and release; negative feedback by circulating thyroid hormones modulates pituitary output. Circadian and developmental factors can influence levels in avian species.

• Research relevance (non-clinical)

  • Quantifying TSH supports studies of endocrine axis dynamics, nutritional and environmental modulation of thyroid activity, and comparative endocrinology in birds.

Note: This section provides general biological context. Item-specific assay performance parameters and validated sample types are not specified; consult the kit documentation.

Buffer Applications

While buffers are not specified for this particular item, ELISA workflows generally rely on well-characterized buffer systems (literature/general):

• Common buffers

  • Coating/Assay buffer: Carbonate–bicarbonate (pH ~9.4) for passive coating, or PBS/TBS (pH 7.2–7.6) for dilutions and antibody incubations.
  • Blocking buffer: Protein-based (e.g., BSA, casein) or polymeric blockers to minimize nonspecific binding; avoid blockers containing analyte-like proteins that may interfere.
  • Wash buffer: PBS or TBS supplemented with surfactant (e.g., 0.05–0.1% Tween 20) to reduce background.
  • Substrate buffer: Proprietary buffers optimized for HRP or AP substrates; protect from light.

• Practical tips

  • Use freshly prepared buffers with high-purity water; filter if particulate is observed.
  • Maintain consistent ionic strength and surfactant concentration across standards and samples.
  • Verify compatibility of blockers/preservatives with the detection enzyme used by the kit.

Item-specific buffer identities, concentrations, and preparation instructions are not specified for this kit; refer to the kit insert for exact recipes and any concentrates included.

Green Alternatives

ELISA workflows are inherently aqueous and typically avoid hazardous organic solvents, aligning with several green chemistry principles. Opportunities for greener practice (general):

• Reduce
  • Scale reactions to the minimum plate area/volume needed; consolidate washes where validated to cut water consumption.
  • Use refillable wash bottles or automated washers with optimized dispense/aspirate cycles.
• Reuse/Recycle
  • Where allowed by institutional policy, collect compatible buffer waste streams for neutralization prior to disposal; segregate substrate-containing waste.
• Replace
  • Prefer non-azide preservatives in waste-sensitive systems if optional; however, specific preservatives in this kit are not specified.

Comparison (general)

• Aqueous ELISA vs solvent-intensive assays:
  • Solvent use: Minimal vs high for some LC/GC sample preps.
  • Energy: Ambient incubations vs high-temperature analytical runs.
  • Waste toxicity: Mild salt solutions vs halogenated solvent waste.

Item-specific environmental attributes (e.g., preservative identity, substrate chemistry) are not specified; refer to the SDS for component-level guidance and local regulations for disposal.

Pharmaceutical Uses

Not typically applicable. This product is a research-use-only ELISA kit intended for laboratory quantification of chicken TSH in research samples and is not designed for clinical diagnostics, therapeutic manufacturing, or as a pharmaceutical excipient.

• General note (literature)
  • ELISA methodologies are employed in pharmaceutical quality control and biomarker research; however, use of a specific kit for regulated applications requires validation under the appropriate quality systems (e.g., GLP/GMP). No such claims are made for this item.

Any pharmacopeial status, IVD claims, or regulatory certifications are not specified for this item.

Physical Properties

This ELISA kit comprises multiple aqueous reagents and solid supports; conventional single-compound physical constants do not apply.

• Item-specific (from Product Data)
  • Appearance: Not specified for this item; refer to CoA/Spec Sheet.
  • Molecular formula and molecular weight: Not applicable to a multi-component kit.
• Typical properties of ELISA reagents (literature/general)
  • Buffers are commonly aqueous (PBS or TBS) with protein stabilizers and preservatives; pH often near physiological (pH ~7.2–7.4).
  • Coated microplates are usually clear polystyrene, 96-well format, high binding capacity.
  • Enzyme substrates (e.g., TMB for HRP-based detection) are light-sensitive and typically supplied as ready-to-use solutions; stop solutions are commonly dilute mineral acids. Specific compositions for this item are not specified.
• Solubility and handling notes (general)
  • All liquid components are water-based and fully miscible with water. Avoid freezing unless the kit insert explicitly permits.
  • Protect chromogenic substrates from light; maintain recommended temperature (2–8°C) to preserve enzyme activity. Exact conditions for this item beyond 2–8°C storage are not specified.

Quality and Grades

• Item-specific (from Product Data)

  • Grade/Purity: BioReagent.

• What BioReagent grade typically implies (general)

  • BioReagent-grade materials are suitable for routine biochemical and immunoassay workflows, emphasizing low bioburden, consistent performance, and compatibility with enzyme-/antibody-based detection. It is not a pharmacopeial or clinical diagnostic grade.
  • For ELISA kits, quality control typically encompasses lot-to-lot consistency checks with reference samples, stability testing at 2–8°C, and verification that background signal and dynamic range meet internal release criteria. Specific QC metrics for this item are not specified; refer to the kit insert/CoA.

• Practical implications

  • Use only the provided reagents and follow the recommended plate maps and incubation times to reproduce validated performance.
  • Do not interchange components across lots or kits, as titering and concentrations are lot-specific in validated immunoassays.

• Documentation

  • For this item, any acceptance criteria (e.g., control range, standard curve characteristics), stabilizers, and component specifications are not specified; refer to the CoA/Spec Sheet and the kit manual for details.

Reaction and Applications

This kit is designed for quantitative immunoassay measurement of chicken TSH by ELISA. While item-specific format details are not provided, the general ELISA workflow and considerations are as follows (literature/general):

• Typical ELISA architecture

  • Sandwich ELISA is commonly used for protein hormones like TSH: capture antibody immobilized on the plate binds analyte; a detection antibody (often enzyme-conjugated or biotinylated followed by streptavidin–enzyme) provides signal. The exact format for this item is not specified.

• Practical tips

  • Equilibration: Bring all reagents to room temperature before use if recommended; avoid repeated freeze–thaw cycles of protein standards.
  • Plate handling: Tap to remove residual wash buffer; use calibrated multichannel pipettes and avoid touching well bottoms.
  • Standard curve: Prepare serial dilutions freshly; mix thoroughly without vortex foaming.
  • Incubations: Maintain consistent times and temperatures; cover plates to prevent evaporation and light-protect chromogens.
  • Matrix effects: Dilute complex matrices (e.g., serum/plasma extracts) to reduce interference; validate dilution linearity for your sample type.

• Applications in research (non-clinical)

  • Endocrine physiology studies in avian species; modulation of pituitary–thyroid axis in nutrition, development, or environmental exposure models.

Item-specific sensitivity, range, and sample compatibility are not specified; refer to the kit insert for validated performance characteristics.

Reaction Conditions

The term “reaction conditions” does not directly apply to this ELISA kit in a synthetic chemistry sense. For immunoassay performance, follow the kit insert. Item-specific incubation times, temperatures, and wash cycles are not specified here.

• General ELISA conditions (literature/general)
  • Temperature: Many ELISA incubations proceed at room temperature (20–25°C) or 37°C, as validated.
  • Timing: Capture/detection incubations often range from 30–120 minutes; substrate development typically 10–30 minutes, with kinetic monitoring as needed.
  • Buffers: PBS/TBS-based assay and wash buffers with surfactant to control background.
  • Signal: HRP/TMB or AP/pNPP systems are common; protect chromogens from light and stop reactions consistently across the plate.

Because validated conditions are kit- and lot-specific, defer to the official protocol provided with this item.

Safety and Handling

• Item-specific (from Product Data)

  • GHS classification, signal word, H-statements, and pictograms: Not specified for this item; refer to the SDS for each component.
  • Storage: Store at 2–8°C; shipped on wet ice.
  • Research use only.

• General safety guidance for ELISA kits (literature/general)

  • Preservatives: Some kit reagents may contain low concentrations of biocidal preservatives (e.g., isothiazolinones) and mineral acids/bases in stop or wash solutions. Avoid skin/eye contact and inhalation of aerosols.
  • PPE: Wear lab coat, safety glasses, and appropriate gloves. Use in a well-ventilated area or biosafety cabinet when handling biological samples.
  • Incompatibilities: Keep enzyme substrates away from strong oxidizers/reductants; avoid metal contamination for peroxidase-based systems. Do not mix components between lots.
  • First aid (overview; defer to SDS): In case of skin/eye contact, rinse with water for at least 15 minutes; if inhaled, move to fresh air; if ingested, rinse mouth and seek medical advice. Show the SDS to medical personnel.
  • Waste: Collect liquid waste containing chromogenic substrates and preservatives as hazardous chemical waste according to institutional regulations.

Always consult the kit insert and component SDS for authoritative safety information.

Solvent Selection

Not typically applicable. This is an aqueous immunoassay kit configured for use with water-based buffers supplied in the kit or specified in the manual.

• General guidance (literature)
  • Avoid organic solvents (e.g., methanol, DMSO) in sample or reagent diluents unless explicitly permitted; they can denature proteins or suppress enzyme activity.
  • If sample matrices contain organic cosolvents, maintain final solvent content at minimal levels (commonly ≤1–2% v/v) to mitigate effects on binding and color development. Exact tolerances for this item are not specified; consult the kit insert.
  • Use high-purity deionized water (≥18 MΩ·cm) to prepare any Wash/Assay buffers if concentrates are provided.

Storage and Reconstitution

• Item-specific (from Product Data)

  • Storage conditions: Store the kit at 2–8°C.
  • Shipping: Shipped on wet ice to maintain cold chain.

• Stability and handling

  • Avoid repeated temperature cycling; return components to 2–8°C promptly after use. Protect light-sensitive reagents from ambient light.
  • Do not freeze components unless the kit insert explicitly instructs; freeze–thaw can degrade antibodies, standards, and enzymes.

• Reconstitution

  • Any reconstitution steps (e.g., lyophilized standards, buffer concentrates) are not specified for this item; refer to the kit manual/labels for volumes, diluents, and stability after reconstitution.

• Post-opening guidance (general)

  • Record first-use date on each bottle/vial; segregate working aliquots if permitted to minimize contamination.
  • Use clean, calibrated pipettes; avoid cross-contamination by changing tips between standards/samples.

Always consult the component labels, Certificate of Analysis, and kit insert for definitive storage and reconstitution instructions.

Structure and Identity

This product is a multi-component immunoassay kit for quantifying chicken Thyroid Stimulating Hormone (TSH); it is not a single, well-defined small molecule.

• Item-specific (from Product Data)
  • Product: Chicken Thyroid Stimulating Hormone (TSH) ELISA Kit (SKU: EJ1515345)
  • Grade: BioReagent
  • Storage: 2–8°C; shipped on wet ice
  • Intended use: For research use only
• Structural identifiers
  • Molecular formula: Not applicable to a kit (no single chemical entity)
  • Molecular weight: Not applicable to a kit
  • SMILES / InChI / InChIKey: Not applicable to a kit
• Target analyte (derived from product name)
  • Analyte: TSH (thyrotropin) from chicken (Gallus gallus)
• General background (literature)
  • TSH is a glycoprotein hormone composed of a common α-subunit and a hormone-specific β-subunit. It stimulates the thyroid gland via the TSH receptor. As a protein analyte, it is heterogeneous (glycosylation), so no single small-molecule identifier applies.
• Kit component expectations (general)
  • Typical ELISA kits may include pre-coated plates, standards, detection antibodies/conjugates, sample/assay buffers, wash buffers, and substrate/stop solutions. Exact component list is not specified for this item; refer to the kit insert.

Synthetic Utility

Not applicable. This is an immunoassay kit for protein quantification and is not used as a synthetic reagent or building block.

• Guidance
  • For chemical synthesis needs (e.g., peptide standards, conjugation reagents), consult relevant catalog sections. This ELISA kit is optimized for biomolecular detection workflows rather than chemical transformations.

Target Specificity

• Item-specific (from Product Data and product name)
  • Analyte/Antigen: Thyroid Stimulating Hormone (TSH; thyrotropin)
  • Species reactivity: Chicken (Gallus gallus)
  • Assay type: ELISA kit for quantitative measurement (research use only)
  • Clone, isotype, and epitope: Not applicable/not specified for this kit; refer to the kit insert if provided.

No additional specificity data (e.g., cross-reactivity, interference) are specified for this item; consult the kit documentation for any characterization data.