ExactAb™, Validated, Carrier Free, 1.0 mg/mL Carrier Free,ExactAb™,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Mouse Monoclonal (2G1D5) to CHK1, could be used for WB and ELISA.
Application:
WB: 1/500 - 1/2000
ELISA: 1/10000
Protein Function:
CHK1, also known as CHEK1, it is a kinase that phosphorylates cdc25, an important phosphatase in cell cycle control, particularly for entry into mitosis. Cdc25, when phosphorylated on serine 216 by chk1 becomes bound by an adaptor protein in the cytoplasm. Therefore it is inhibited from removing the inhibiting phosphate from MPF (mitotic/maturation promoting factor) added by Wee1. Consequently, a cell is prevented from entering mitosis. Chk1 can also phosphorylate p53 at Ser20 in vitro.
Chk1 Mouse mAb (Ab096310) - Western Blot
All lanes: Chk1 Mouse mAb (Ab096310) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution
Predicted band size: 56 kDa
Observed band size: 56 kDa
Chk1 Mouse mAb (Ab096310) - Western Blot
All lanes: Chk1 Mouse mAb (Ab096310) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution
Predicted band size: 56 kDa
Observed band size: 56 kDa
| Applicazione | Dilution info |
|---|---|
| WB | 1/500-1/2000 |
| ELISA |
1/10000
|
Comprehensive hazard, handling, storage, and regulatory compliance document.
Download SDS →Lot-specific quality data. Enter your lot number to retrieve the exact COA.
Look up COA →Full quality attributes and acceptance criteria for this grade.
View spec sheet →| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Data | Oggetto |
|---|---|---|---|
 ZJ23F0700740 | Certificate of Analysis | May 19, 2026 | Ab096310 |
| ZJ23F0700741 | Certificate of Analysis | May 19, 2026 | Ab096310 |
| ZJ26F0737181 | Certificate of Analysis | May 19, 2026 | Ab096310 |
| ZJ24F0303042 | Certificate of Analysis | Mar 08, 2024 | Ab096310 |
Our grade selection guide covers purity, stabilizer status, and application suitability for all variants in our catalog.
View Carrier Free grade guide → View Validated grade guide → View ExactAb™ grade guide →
