CMP-sialic acid synthetase (NmCSS), CAS No.9067-82-7

CAS: 9067-82-7 Cat. No.: C1524613

GRADE & PURITY Bioactive, Recombinant, EnzymoPure™, ≥100 U/mg protein; Protein concentration: See COA

Accession #

P0A0Z8

Protein Tag

C-His

Expression system

E. coli

Bioactivity

≥100 U/mg protein

Panoramica

CMP–sialic acid synthetase (NmCSS), EC 2.7.7.43, derived from Neisseria meningitidis. This enzyme activates sialic acid to produce CMP-sialic acid, which serves as the sialic acid donor for glycosyltransferases, and acts as a tool enzyme for sialylation transfer reactions. This product is recombinantly expressed in Escherichia coli, featuring high purity and excellent compatibility, and is applicable in one-pot multi-enzyme systems.

Reaction Equation:

Neu5Ac + CTP → CMP-Neu5Ac + PPi

Instructions for Use

Add substrates (Neu5Ac and CTP) and 5 mM MgCl₂ to the reaction system; appropriate buffer salts (e.g., Tris-HCl) may be supplemented to adjust the pH to 7.5–8.5, followed by the appropriate amount of NmCSS according to reaction requirements. Addition of inorganic pyrophosphatase to the system can improve conversion efficiency.

Mix thoroughly and incubate the sample at 37 °C for 30–60 min.

Notes

When scaling up the reaction system, incubation time or enzyme dosage shall be adjusted according to practical conditions; low-speed shaking mixing is recommended.

Most common biological buffers such as Tris and PBS are compatible. Buffers outside this pH range (e.g., acetate buffer) may also be usable, but incubation time and enzyme dosage need to be optimized on a case-by-case basis.

Precautions

For your safety and health, please wear a lab coat and disposable gloves during handling. This product is for research use only!

Specifications

Product Name

CMP-sialic acid synthetase (NmCSS), CAS No.9067-82-7

Grado

Bioactive, Recombinant, EnzymoPure™

Specifiche e purezza

Bioactive, Recombinant, EnzymoPure™, ≥100 U/mg protein; Protein concentration: See COA

Bioattività

≥100 U/mg protein

Numero d'ordine

P0A0Z8

Peso molecolare previsto

26 kDa

CAS

9067-82-7

Numero della Commissione Enzimi

EC 2.7.7.43

Tipo di molecola

Enzima

Stoccaggio e spedizione

Concentrazione

≥100 U/mg protein; Protein concentration: See COA

Condizioni di conservazione di stoccaggio

Store at -20°C,Avoid repeated freezing and thawing

Spedito in

Ice chest + Ice pads

Stabilità e conservazione

Store at -20℃ long term (18 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Definizione dell'unità

The amount of enzyme that catalyzes the formation of 1 μmol CMP-sialic acid from sialic acid and CTP per minute at 37 ℃ and pH 8.0.

Domande frequenti

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What are the CAS number, molecular formula and molecular weight?

The CAS Number is 9067-82-7.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

What does Bioactive mean?

Bioactive indicates that the product has been processed and tested for biological and biochemical applications. Specifications may include endotoxin limits, microbial controls, sterility, low DNase/RNase/protease activity, and verified biological activity where relevant.

What does Bioactive mean?

Bioactive indicates that the product has been processed and tested for biological and biochemical applications. Specifications may include endotoxin limits, microbial controls, sterility, low DNase/RNase/protease activity, and verified biological activity where relevant.

What does Recombinant mean?

Recombinant is a defined analytical purity tier suitable for workflows requiring a specific validated property. It specifies a minimum assay percentage, controlled limits on common impurities (water, heavy metals, residue on ignition), and lot-to-lot consistency. Recombinant is one of several purity tiers — choose based on the sensitivity and regulatory requirements of your method.