1. Scope of Application
| Application | Category |
|---|
| Selective isolation | Clostridium |
|
2. Principles and Uses
Trypticase (pancreatic digest of casein), peptic digest of meat, and pancreatic digest of heart provide nitrogen sources, growth factors, minerals, amino acids, etc. Yeast extract powder and corn starch supply vitamins, especially B‑group vitamins essential for bacterial growth. Sodium chloride maintains osmotic equilibrium. Agar is the solidifying agent.
3. Formula (g/L)
| Trypticase (pancreatic digest of casein) | 10.0 g | | Peptic digest of meat | 5.0 g | | Pancreatic digest of heart | 3.0 g | | Yeast extract powder | 5.0 g | | Corn starch | 1.0 g | | Sodium chloride | 5.0 g | | Agar | 15.0 g | | Final pH (25°C) | 7.3 ± 0.2 |
|
4. Preparation Method
Weigh 44.0 g of the medium and transfer to 1 L of purified water. Heat to boiling with frequent agitation until completely dissolved. Dispense into suitable containers and autoclave at 121°C for 15 min. Cool to 45–50°C. Under aseptic conditions, add 1 mL of gentamicin sulfate solution (KS0005, 2 mg/mL) per 100 mL of medium, mix well, and pour into Petri dishes for use.
5. Quality Control
| Test Item | Specification |
|---|
| Appearance (dry powder) | Powder | | Color (dry powder) | Yellow | | Color (prepared medium) | Light yellow, transparent solid | | Final pH (25°C) | 7.3 ± 0.2 |
|
6. Microbiological Testing
Tested according to the Chinese Pharmacopoeia using Clostridium sporogenes.
| Quality Control Strain & Number | Growth Performance / Colony Characteristics |
|---|
| Clostridium sporogenes CMCC(B)64941 | Good growth, colorless colonies |
|
7. Storage Conditions
Store at room temperature in a sealed, dry container, protected from light.
Shelf life: 3 years.
8. Reference Standard
Chinese Pharmacopoeia
9. Precautions
1.When weighing the medium, take necessary protective measures to avoid discomfort.
2.Store the medium in a dry place protected from light. After use, immediately tighten the cap to prevent moisture absorption and caking.
3.Used medium should be autoclaved at 121°C for 30 minutes before disposal.
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