Cre mRNA - BioReagent,Integrity of mRNA ≥85% (CE);1.0 mg/mL

Cat. No.: M1506461

GRADE & PURITY BioReagent, Integrity of mRNA ≥85% (CE);1.0 mg/mL

Storage

Store at -20°C,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Panoramica

Storage buffer: 1 mM Sodium citrate, pH 6.5

Cre recombinase (Cyclization Recombinase) is a protein composed of 343 amino acids derived from bacteriophage P1. It is a member of the tyrosine site-specific recombinase family and can catalyze recombination reactions between specific recognition sequences on DNA. Cre recombinase can bind to loxP sites on DNA and perform precise cutting and ligation operations between these sites, thereby leading to deletion, inversion, or translocation of DNA fragments. 

This product uses a 5'Cap 1 structure with a capping efficiency of ≥95%, and its 3' end is designed with a poly(A) tail of approximately 100nt in length. Meanwhile, Cre mRNA encodes a Cre protein with N-terminal and C-terminal nuclear localization signals (NLS). Introducing two NLS signals into the mRNA can increase the frequency of its delivery to the nucleus, thereby improving DNA editing efficiency.

Product Attributes

Product Concentration:	1mg/mL
Storage Solution:	1 mM Sodium Citrate, pH6.5
Full Length of mRNA Sequence:	1442nt
Integrity:	≥85%
Product Purity:	Single mRNA Electrophoresis Band
Transfection Efficiency:	293T Cell Transfection

Transportation and Storage

This product is transported with dry ice and can be stored at -20℃~-80℃ for two years.

Precautions

• It can be dissolved at room temperature. After dissolution, it should be stored in an ice box or on an ice bath. During use, be careful to prevent RNase contamination and degradation. After use, it should be immediately stored at  -20℃~-80℃.
• This product is for scientific research use only, and shall not be used for clinical diagnosis or treatment, nor for food or drugs.
• For your safety and health, please wear a lab coat and disposable gloves during operation.

Specifications

Specifiche e purezza

BioReagent,Integrity of mRNA ≥85% (CE);1.0 mg/mL

Stabilità e conservazione

Store at -20℃~-80℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Condizioni di conservazione di stoccaggio

Store at -20°C,Avoid repeated freezing and thawing

Spedito in

Ice chest + Ice pads

Questo prodotto richiede spedizione a catena fredda. I servizi di terra e altri servizi economici non sono disponibili.

Grado

BioReagent

Domande frequenti

What does BioReagent mean?

BioReagent indicates that the product has been processed and tested for biological and biochemical applications. Specifications may include endotoxin limits, microbial controls, sterility, low DNase/RNase/protease activity, and verified biological activity where relevant.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.