Cryptosporidium Oocyst Staining Solution (Auramine-Phenol Method) - BioReagent,Suitable for microbiology,Biological Stain

Cat. No.: S1510269

GRADE & PURITY BioReagent, Suitable for microbiology, Biological Stain

Synonyms

Cryptosporidium Oocyst Stain (Auramine-Phenol Method)

Storage

Protected from light,Room temperature

Shipped In

Normal

Panoramica

Staining methods for the detection of Cryptosporidium oocysts include the auramine-phenol staining method and the modified acid-fast staining method, among others. The Auramine-Phenol Staining Solution is composed of auramine and phenol, and is suitable for staining Cryptosporidium oocysts in fresh fecal specimens or specimens fixed with formalin. This reagent is for research use only and is not suitable for clinical diagnosis or other purposes.

S1510269	Component	3×20 mL	Storage
S1510269A	Auramine-Phenol Staining Solution	20 mL	RT. Store in the dark.
S1510269B	Differentiation Solution	20 mL	RT.
S1510269C	Oxidizing Solution	20 mL	RT. Store in the dark.

Operating Steps (For Reference Only)

1. Prepare a thin fecal smear on a glass slide and allow it to air dry. Fix with methanol for 2–3 minutes.

2. Apply Auramine-Phenol Staining Solution onto the air-dried fecal film and let stand for 10–15 minutes. Rinse with water.

3. Apply Differentiation Solution onto the fecal film and let stand for 1 minute. Rinse with water.

4. Apply Oxidizing Solution onto the fecal film and let stand for 1 minute. Rinse with water.

5. Allow the slide to dry and examine under a fluorescence microscope.

Staining Results

• Under low magnification fluorescence microscopy, oocysts appear as small, round bright spots emitting a milky white fluorescence.

• Under high magnification fluorescence microscopy, oocysts appear milky white or slightly greenish. The oocyst wall is thin, and most oocysts show a deeply stained periphery with a paler center, giving a ring‑like appearance.

Precautions

1. Some specimens may show non‑specific fluorescent particles; careful differentiation is required.

2. For your safety and health, please wear a lab coat and disposable gloves during operation.

3. Please use the reagent as soon as possible after opening to avoid affecting subsequent experimental results.

Specifications

Sinonimi

Cryptosporidium Oocyst Stain (Auramine-Phenol Method)

Specifiche e purezza

BioReagent,Suitable for microbiology,Biological Stain

Stabilità e conservazione

Store at room temperature long term (12 months). Store in the dark.

Condizioni di conservazione di stoccaggio

Protected from light,Room temperature

Spedito in

Normal

Grado

Biological Stain, BioReagent, Suitable for microbiology

Proprietà chimiche e fisiche

• Sensibilità: Light-sensitive

Domande frequenti

How is this product shipped?

This product ships under standard ambient conditions. No temperature-controlled packaging is required.

What does Suitable for microbiology mean?

Suitable for microbiology indicates that the product has been processed and tested for extraction workflows and microbiology. Specifications may include endotoxin limits, microbial controls, sterility, low DNase/RNase/protease activity, and verified biological activity where relevant.

What does BioReagent mean?

BioReagent indicates that the product has been processed and tested for biological and biochemical applications. Specifications may include endotoxin limits, microbial controls, sterility, low DNase/RNase/protease activity, and verified biological activity where relevant.

How should this product be stored?

Store at room temperature, protected from light. The material is light-sensitive — keep it in its original opaque or amber container.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.