DAF-2 - ≥95%(HPLC) , CAS No.205391-01-1

CAS: 205391-01-1 Cat. No.: D131457 Formula: C₂₀H₁₄N₂O₅ Peso molecolare: 362.34 Numero EC: 634-985-9

GRADE & PURITY ≥95%(HPLC)

Synonyms

4,5-Diaminofluorescein | 2-(3,6-Dihydroxy-9H-xanthen-9-yl)-4,5-diamino-benzoic acid | DTXSID60443252 | AS-78359 | 5,6-Diamino-3',6'-dihydroxy-3H-spiro[isobenzofuran-1,9'-xanthen]-3-one | DAF-2-CAS 205391-01-1-Calbiochem | Spiro[isobenzofuran-1(3H),9'-[9H]

Storage

Store at -20°C

Shipped In

Ice chest + Ice pads

Panoramica

DAF-2 is a sensitive and specific fluorescent indicator for the direct detection of nitric oxide (NO) in vitro. In the presence of oxygen, the relatively non-fluorescent DAF-2 reacts rapidly with NO to yield the highly fluorescent triazolofluorescein compound. DAF-2 can detect NO under neutral conditions (detection limit = 5 nM).
A sensitive and specific fluorescent indicator for the direct detection of nitric oxide (NO) in vitro.

Specifications

Sinonimi

4,5-Diaminofluorescein | 2-(3,6-Dihydroxy-9H-xanthen-9-yl)-4,5-diamino-benzoic acid | DTXSID60443252 | AS-78359 | 5,6-Diamino-3',6'-dihydroxy-3H-spiro[isobenzofuran-1,9'-xanthen]-3-one | DAF-2-CAS 205391-01-1-Calbiochem | Spiro[isobenzofuran-1(3H),9'-[9H]

Specifiche e purezza

≥95%(HPLC)

Meccanismi biochimici e fisiologici

Highly sensitive, specific fluorescent detector for nitric oxide (NO) . Essentially nonfluorescent until it reacts with NO to yield the highly fluorescent triazolofluorescein (DAF-2T).

Condizioni di conservazione di stoccaggio

Store at -20°C

Spedito in

Ice chest + Ice pads

Questo prodotto richiede spedizione a catena fredda. I servizi di terra e altri servizi economici non sono disponibili.

Nota

Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour. Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.

Purezza

≥95%(HPLC)

Nomi e identificatori

Pubchem Sid

504765573

Pubchem Sid Url

https://pubchem.ncbi.nlm.nih.gov/substance/504765573

Sorrisi canonici

C1=CC2=C(C=C1O)OC3=C(C24C5=CC(=C(C=C5C(=O)O4)N)N)C=CC(=C3)O

IUPAC Name

5,6-diamino-3',6'-dihydroxyspiro[2-benzofuran-3,9'-xanthene]-1-one

InChIKey

LTYUPYUWXRTNFQ-UHFFFAOYSA-N

INCHI

1S/C20H14N2O5/c21-15-7-11-14(8-16(15)22)20(27-19(11)25)12-3-1-9(23)5-17(12)26-18-6-10(24)2-4-13(18)20/h1-8,23-24H,21-22H2

Isomeri SMILES

C1=CC2=C(C=C1O)OC3=C(C24C5=CC(=C(C=C5C(=O)O4)N)N)C=CC(=C3)O

Peso molecolare

362.34

Reaxy-Rn

8014486

Reaxys-RN_link_address

https://www.reaxys.com/reaxys/secured/hopinto.do?context=S&query=IDE.XRN=8014486&ln=

Taxonomic Classification

Taxonomy Tree

• Kingdom Organic compounds
  • Superclass Organoheterocyclic compounds
    • Classe Benzopyrans
      • Subclass 1-benzopyrans
        • Level5 Dibenzopyrans
          • Level6 Xanthenes

• Kingdom: Organic compounds
• Superclass: Organoheterocyclic compounds
• Classe: Benzopyrans
• Subclass: 1-benzopyrans
• Intermediate Tree Nodes: Dibenzopyrans
• Direct Parent: Xanthenes
• Alternative Parents: Diarylethers Phthalides Benzofuranones Isobenzofurans 1-hydroxy-2-unsubstituted benzenoids Lactones Carboxylic acid esters Amino acids and derivatives Oxacyclic compounds Primary amines Organic oxides Hydrocarbon derivatives
• Molecular Framework: Aromatic heteropolycyclic compounds
• Substituents: Xanthene - Diaryl ether - Benzofuranone - Phthalide - Isobenzofuranone - Isocoumaran - Isobenzofuran - Phenol - 1-hydroxy-2-unsubstituted benzenoid - Benzenoid - Carboxylic acid ester - Lactone - Amino acid or derivatives - Ether - Carboxylic acid derivative - Oxacycle - Hydrocarbon derivative - Organooxygen compound - Organonitrogen compound - Organic oxide - Organic nitrogen compound - Organic oxygen compound - Amine - Primary amine - Aromatic heteropolycyclic compound
• Descrizione: This compound belongs to the class of organic compounds known as xanthenes. These are polycyclic aromatic compounds containing a xanthene moiety, which consists of two benzene rings joined to each other by a pyran ring.
• External Descriptors: Not available

Data sources

1. Djoumbou Feunang Y, Eisner R, Knox C, Chepelev L, Hastings J, Owen G, Fahy E, Steinbeck C, Subramanian S, Bolton E, Greiner R, and Wishart DS. ClassyFire: Automated Chemical Classification With A Comprehensive, Computable Taxonomy. Journal of Cheminformatics, 2016, 8:61.

DOI: 10.1186/s13321-016-0174-y

Proprietà chimiche e fisiche

• Peso molecolare: 362.300 g/mol
• XLogP3: 2.100
• Hydrogen Bond Donor Count: 4
• Hydrogen Bond Acceptor Count: 7
• Rotatable Bond Count: 0
• Exact Mass: 362.09 Da
• Monoisotopic Mass: 362.09 Da
• Topological Polar Surface Area: 128.000 Ų
• Heavy Atom Count: 27
• Formal Charge: 0
• Complexity: 589.000
• Isotope Atom Count: 0
• Defined Atom Stereocenter Count: 0
• Undefined Atom Stereocenter Count: 0
• Defined Bond Stereocenter Count: 0
• Undefined Bond Stereocenter Count: 0
• The total count of all stereochemical bonds: 0
• Covalently-Bonded Unit Count: 1