DGKB Mouse mAb - Anticorpo primario, specific to DGKB

Cat. No.: Ab099751

QUALITÀ & PUREZZA See COA

Application

WB

Host species

Mouse

Reactive species

consiglio, Mouse, Scimmia, Tuttavia, Umano

Isotype

Mouse IgG1

Clone Number

OTI1F9

Conjugation

Unconjugated

Panoramica

Mouse anti Human DGKB Antibody, Monoclonal (OTI1F9), could be used for WB and so on.

Specifiche

Product Name

DGKB Mouse mAb - Anticorpo primario, specific to DGKB

Sinonimi

90 kDa diacylglycerol kinase antibody | DAG kinase beta antibody | DAGK2 antibody | DGK antibody | DGK B antibody | DGK beta antibody | DGK-beta antibody | Dgkb antibody | DGKB_HUMAN antibody | Diacylglycerol kinase beta (90kD) antibody | Diacylglycerol k

Specifiche e purezza

See COA

Meccanismi biochimici e fisiologici

Diacylglycerol kinase that converts diacylglycerol/DAG into phosphatidic acid/phosphatidate/PA and regulates the respective levels of these two bioactive lipids. Thereby, acts as a central switch between the signaling pathways activated by these second me

Specie ospite

Mouse

Specificità

DGKB

Immunogeno

Recombinant full length protein of human DGKB produced in HEK293T cell.

Controllo positivo

WB: HEK293T transfected with pCMV6-ENTRY DGKB cDNA for 48 hrs; HepG2, HeLa, HT29, A549, COS7, Jurkat, MDCK, PC12, MCF7 lysates.

Coniugazione

Unconjugated

SDS-PAGE

150 kDa

Concentrazione

See COA

Product Line

Prodotti biologici

Stoccaggio e spedizione

Forma

Liquid

Condizioni di conservazione di stoccaggio

Store at -20°C,Avoid repeated freezing and thawing

Spedito in

Ice chest + Ice pads

Stabilità e conservazione

Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Applicazione

Applicazione

Dilution info

WB

1/500-1/2000

Obiettivi associati (umani)

DGKB Tbio Diacylglycerol kinase beta (0 Activities)

Discover Target: DGKB

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Application Protocols

Tested applications, recommended dilutions, and positive control samples are not specified for this item; refer to the CoA/Spec Sheet.

General workflow guidance (literature/general, not item-specific):

• Western blot: Prepare lysates with appropriate detergents, load equal protein amounts, transfer to PVDF/nitrocellulose, block (e.g., 5% BSA), incubate with primary antibody, wash, incubate with species-matched secondary, develop via chemiluminescence or fluorescence.
• Immunocytochemistry/Immunofluorescence: Fix/permeabilize cells (e.g., PFA + Triton X-100 as appropriate), block, incubate with primary, wash, incubate with fluorophore-labeled secondary, mount with antifade medium.
• Immunohistochemistry (FFPE): Perform antigen retrieval (buffer and temperature optimized per tissue/epitope), block endogenous enzymes/biotin if needed, apply primary, detect with HRP/AP polymer systems, counterstain.
• ELISA: Coat antigen, block, apply primary antibody, wash, detect with enzyme-conjugated secondary and substrate; or use capture/detection pairs in sandwich formats.

Important: Optimize antibody concentration, incubation time, and buffer composition empirically. Always confirm item-specific recommendations and tested applications on the product CoA/Spec Sheet.

Biological Roles

DGKB (diacylglycerol kinase beta) is an enzyme that phosphorylates diacylglycerol (DAG) to generate phosphatidic acid (PA), regulating lipid second messenger balance.

• Functional context (literature/general):

  • DGK family enzymes terminate DAG signaling (e.g., PKC activation) and produce PA, which can act as a signaling lipid and a precursor in glycerophospholipid biosynthesis.
  • DGKB is expressed in neural tissues and other cell types, where it contributes to synaptic signaling, cytoskeletal dynamics, and membrane trafficking via lipid signaling pathways.
  • Subcellular localization has been reported at the plasma membrane and cytosol; localization can be stimulus-dependent (literature reports vary by cell type and isoform).

• Pathway relevance (literature/general):

  • DAG–PA interconversion influences PLC/PKC pathways, mTOR signaling (via PA), and membrane curvature/stress responses.
  • Alterations in DGK activity can modulate receptor signaling thresholds and downstream transcriptional outputs.

Use of DGKB Mouse mAb:

• This antibody targets DGKB and can be incorporated into research workflows that probe DGKB abundance, localization, or interaction networks. Item-specific validation data, reactive species, and application suitability (e.g., WB, IHC, IF, ELISA) are not specified for this product; verify with CoA/Spec Sheet and perform application-specific optimization.

Buffer Applications

As a monoclonal antibody reagent, DGKB Mouse mAb is commonly used in standard immunoassay buffers rather than serving as a buffering component itself.

General buffer systems for antibody workflows (literature/general):

• Sample and dilution buffers: PBS or TBS at neutral pH (7.2–7.6) with optional protein carriers (e.g., 0.1–1% BSA) and mild detergents (e.g., 0.05% Tween-20) to reduce nonspecific interactions.
• Blocking buffers: 3–5% BSA, casein, or serum (species-compatible) depending on application (WB, IHC, IF); milk-based blockers can contain biotin and phosphoproteins that interfere with some assays.
• Wash buffers: PBS-T or TBS-T to balance stringency and epitope preservation.
• Antigen retrieval (IHC, if applicable): Citrate (pH ~6) or Tris-EDTA (pH ~9) buffers may be used depending on fixation; optimization is target- and tissue-dependent.

Item-specific buffer compatibility, recommended blockers, and preservative content are not specified for this item; consult the CoA/Spec Sheet and validate in your assay context.

Green Alternatives

While “green alternatives” are typically discussed for organic solvents and small-molecule reagents, sustainability considerations for antibodies focus on formulation and logistics.

• Formulation and handling (general):

  • Use concentrated stocks and small, application-appropriate aliquots to reduce waste from freeze–thaw degradation.
  • Choose azide-free formulations when necessary to facilitate environmentally safer disposal (where compatible with your assays and bioburden control).

• Logistics and packaging:

  • Consolidate shipments and select slower, cold-chain-stable transport when project timelines allow to reduce expedited-shipping footprint.
  • Reuse or recycle cold packs and insulated shippers where institutional policies permit.

• Alternative reagent strategies (general):

  • Consider recombinant antibodies or recombinant binding scaffolds (e.g., scFv, nanobodies) that can be produced in microbial systems, potentially lowering animal use and environmental impact.

Item-specific eco-profile (e.g., preservative type, concentration, packaging details) is not specified for this item; consult the CoA/Spec Sheet if such details are critical to your sustainability program.

Pharmaceutical Uses

This product is labeled for research use only (RUO) and is not intended for diagnostic, therapeutic, or manufacturing use in humans or animals.

• Excipient/formulation status: Not specified for this item; no pharmacopeial status is claimed.
• Potential roles in pharma R&D (literature/general):
  • Tool antibody for target validation, mechanism-of-action studies, and biomarker discovery related to DGKB signaling in preclinical research settings.
  • Reagent in immunoassays that support discovery workflows (e.g., screening changes in DGKB expression in cell-based models).

Any use in GMP environments, regulated bioanalytical assays, or clinical manufacturing would require additional qualification and is outside the scope of this product as provided.

Physical Properties

• Appearance/formulation: Not specified for this item; refer to CoA/Spec Sheet.
• Concentration: Not specified for this item; refer to CoA/Spec Sheet.
• Molecular weight: Not specified for this item; for a generic mouse IgG, a literature-typical value is ~150 kDa (heavy chain ~50 kDa, light chain ~25 kDa each).
• Isoelectric point (pI): Not specified for this item; IgG pI commonly ranges ~6–9 (literature, clone- and isotype-dependent).
• Extinction coefficient (A280): Not specified for this item; typical IgG E(1%, 280 nm) ≈ 13–14 (literature) but varies with sequence and glycosylation.
• Solubility: Antibodies are water-soluble proteins; high solubility in buffered aqueous solutions (e.g., PBS, TBS) with stabilizers (literature/general). Specific solubility and buffer composition are not specified for this item.
• Storage buffer components (e.g., carrier protein, preservative): Not specified for this item; refer to CoA/Spec Sheet.

Notes:

• All item-specific physical property values (buffer composition, concentration, excipients) are not provided and should be confirmed on the product label or CoA. Literature values above are provided for context and must not be used as specifications.

Quality and Grades

• Grade/Purity: Not specified for this item; refer to CoA/Spec Sheet.
• Classification: Supplied as a research-use-only (RUO) monoclonal antibody.

What RUO implies (general):

• Manufactured and QC-tested for laboratory research applications, not for in vitro diagnostic or clinical use.
• Typical QC for mAbs may include SDS-PAGE purity, binding specificity by ELISA or immunoassay, and functional performance in selected applications—however, specific tests for this item are not specified here.

Stabilizers and preservatives (item-specific status):

• Presence/absence of carrier proteins (e.g., BSA), cryoprotectants (e.g., 10–50% glycerol), or preservatives (e.g., 0.02–0.05% sodium azide) is not specified for this item; consult the product label/CoA as these impact storage, enzyme conjugation, and certain assays.

Batch documentation:

• For clone designation, isotype, epitope mapping, species reactivity, and validated applications, refer to the lot-specific Certificate of Analysis (CoA) or technical data sheet.

Practical note:

• If low background and consistent performance are critical (e.g., quantitative immunoassays), request lot-specific data and consider performing application-specific titration upon receipt.

Reaction and Applications

This section typically covers chemical reaction use-cases, which do not apply to monoclonal antibodies. DGKB Mouse mAb is intended for immunoassay and protein-detection workflows rather than as a reagent in synthetic chemistry.

• Relevant content for this product is provided under Biological Roles, Target Specificity, Application Protocols, and Storage & Reconstitution.
• If you require antibody conjugation (e.g., HRP, biotin, fluorophores), standard protein conjugation chemistries (e.g., NHS–ester labeling of lysines, maleimide–thiol coupling) may be applicable in principle; however, conjugation protocols and performance are not specified for this item and should be validated in-house.

Reaction Conditions

Not applicable to chemical synthesis. For immunoassay workflows using antibodies, operational conditions are assay-specific and must be optimized experimentally.

General operational ranges (literature/general, not item-specific):

• Incubation: Commonly at room temperature or 4°C; times vary from 1 h to overnight depending on assay (WB, IHC, IF, ELISA) and antibody affinity.
• Buffers: Neutral pH (7.2–7.6) PBS/TBS, with or without mild detergents; blocking proteins to reduce background as needed.
• Antigen retrieval (IHC): Heat-induced protocols in citrate or Tris-EDTA buffers when formalin-fixed paraffin-embedded tissues are used; conditions must be titrated for DGKB epitopes.

This product’s validated applications, optimal temperatures, incubation times, and dilutions are not specified; consult CoA/Spec Sheet and perform empirical optimization.

Safety and Handling

• Research use: For research use only (RUO); not for diagnostic or therapeutic use.
• GHS classification, signal word, hazard statements, pictograms: Not specified for this item; consult the SDS for authoritative safety information.

General guidance for handling protein antibodies (literature/general):

• PPE: Wear lab coat, gloves, and safety glasses; avoid aerosol generation and ingestion. Handle as a biological protein solution using good microbiological practices.
• Incompatibilities: Avoid strong oxidizers and extreme pH or temperatures that can denature proteins. Azide-containing buffers can be incompatible with HRP-based assays; check formulation before use.
• First aid (general): If skin/eye contact occurs, rinse with water. If inhaled as aerosol, move to fresh air. If swallowed, rinse mouth. Seek medical attention if symptoms persist. Always follow institutional biosafety procedures.
• Spill/cleanup: Absorb with disposable towels, wash area with suitable detergent; dispose of waste per local regulations.
• Special risks: Repeated freeze–thaw cycles can cause aggregation and loss of activity. Maintain cold chain on ice during handling, and aliquot upon first thaw to minimize freeze–thaw.

Storage and shipping (item-specific):

• Storage Conditions: Store at -20°C. Avoid repeated freezing and thawing.
• Shipped In: Ice chest + Ice pads.

Solvent Selection

This product is a protein antibody and is not used as a reaction solvent. The relevant consideration is the choice of aqueous buffer for dilution and storage.

• Suitable diluents (general/literature):

  • PBS (phosphate-buffered saline, pH ~7.2–7.4) or TBS (Tris-buffered saline, pH ~7.4–8.0) are common for immunoassays.
  • Additives such as 0.05–0.1% Tween-20 can reduce nonspecific binding in wash buffers; bovine serum albumin (BSA) or casein can serve as blocking/stabilizing proteins where compatible.
  • For long-term storage and freeze protection, 10–50% glycerol is often used (check enzyme conjugation compatibility and azide content as needed).

• When to choose PBS vs TBS (general):

  • PBS favors phospho-sensitive targets only if compatible; some phospho-epitopes may prefer TBS to reduce ionic interference. TBS is often preferred for alkaline phosphatase-based detection to avoid phosphate.

• Incompatibilities:

  • Sodium azide inhibits HRP and many microbial oxidases; avoid azide in HRP-mediated detection. Tris can interfere with certain crosslinking chemistries. High detergent levels may disrupt antigen–antibody interactions.

Item-specific buffer composition and recommended diluents are not specified for this item; refer to CoA/Spec Sheet.

Storage and Reconstitution

• Storage (item-specific): Store at -20°C. Avoid repeated freezing and thawing. Shipped with ice chest + ice pads to maintain cold chain.

• Form/Buffer: Not specified for this item; refer to CoA/Spec Sheet for formulation details (e.g., buffer composition, carrier proteins, glycerol, preservatives).

• Aliquoting and handling (general/literature):

  • Upon first receipt, keep cold, briefly spin to collect contents, and prepare single-use aliquots to prevent multiple freeze–thaw cycles.
  • Thaw on ice; mix gently by inversion—avoid vigorous vortexing to limit aggregation.
  • For long-term storage, many antibodies are stable at -20°C or -80°C, often with 10–50% glycerol; confirm compatibility with downstream assays and the product’s actual formulation before adding any excipient.

• Reconstitution: If supplied lyophilized (status not specified), reconstitute with sterile, antibody-grade water or buffer as directed on the vial label to the desired concentration, allow to equilibrate on ice, and gently mix until fully dissolved. If supplied in liquid form, do not dilute stock unnecessarily; prepare working solutions fresh.

• Freeze–thaw guidance: Minimize freeze–thaw by aliquoting; avoid frost-free freezers. Track aliquot freeze dates and discard if performance declines.

Always consult the item’s CoA/Spec Sheet for exact storage format, buffer composition, and any stabilizers or preservatives present.

Structure and Identity

DGKB Mouse mAb is a mouse-derived monoclonal antibody raised against DGKB (diacylglycerol kinase beta). As a protein biologic, it is not represented by small-molecule identifiers.

• Item-specific identifiers:

  • Target antigen: DGKB (diacylglycerol kinase beta)
  • Host: Mouse (monoclonal antibody)
  • Clone, isotype, light chain type: Not specified for this item; refer to CoA/Spec Sheet.
  • CAS, CID, InChIKey, SMILES: Not applicable to antibody biologics.

• General antibody structural features (literature/general):

  • Typical IgG architecture: two heavy and two light chains (H2L2), each with variable (V) and constant (C) domains connected by disulfide bonds.
  • Approximate molecular mass: ~150 kDa for an intact IgG (literature typical value; not item-specific).
  • Antigen-binding sites are located in the complementarity-determining regions (CDRs) of the Fab fragments; Fc region mediates effector interactions and dictates isotype-specific properties.

• 2D description in words (general):

  • Y-shaped heterotetrameric glycoprotein with two identical Fab arms (antigen binding) and one Fc stem (effector domain); N-linked glycans on Fc (and sometimes Fab) depending on expression system.

Synthetic Utility

Not applicable. DGKB Mouse mAb is a protein reagent for immunoassays and target detection, not a synthetic building block or catalyst.

• For conjugation chemistry (general/literature): If end-user conjugation is desired (e.g., biotinylation, fluorophore coupling), lysine-directed NHS-ester labeling or cysteine-selective maleimide chemistry are commonly employed. Buffer exchange into amine-free buffers (e.g., PBS without Tris, pH ~7.2–7.5) and removal of azide are typical prerequisites. Such procedures and performance outcomes are not specified for this item and must be validated by the user.

Target Specificity

• Antigen: DGKB (diacylglycerol kinase beta)
• Epitope: Not specified for this item; refer to CoA/Spec Sheet.
• Clone: Not specified for this item; refer to CoA/Spec Sheet.
• Isotype/Light chain: Not specified for this item; refer to CoA/Spec Sheet.
• Species reactivity (tested/expected): Not specified for this item; refer to CoA/Spec Sheet.

Literature background on DGKB:

• Gene/protein: DGKB is a member of the diacylglycerol kinase family that converts DAG to PA, modulating lipid signaling.
• Cellular localization: Cytosol and plasma membrane; stimulus- and cell type–dependent redistribution has been reported (literature).
• Orthology: Conserved across mammals; homology may enable cross-reactivity depending on epitope conservation, but cross-reactivity for this item is not specified and must be empirically determined.

Recommendation:

• Verify species compatibility, epitope accessibility (native vs denatured), and application suitability (WB/IHC/IF/ELISA) from the item’s CoA/Spec Sheet before experimental use.