Di-4-ANEPPDHQ (Membrane Potential Fluorescent Probe) - BioReagent, Biological Stain, for Fluorescence analysis, for Microscopy, ≥98% , CAS No.797785-10-5

CAS: 797785-10-5 Cat. No.: D1455331 Formula: C₃₂H₄₇Br₂N₃O₂ Peso molecolare: 665.54

GRADE & PURITY BioReagent, Biological Stain, for Fluorescence analysis, for Microscopy, ≥98%

Storage

Protected from light,Store at -20°C,Desiccated

Shipped In

Ice chest + Ice pads

Application

Cell Staining‌, Fluorescence Analysis

Panoramica

Di‑4‑ANEPPDHQ is a dye that rapidly responds to changes in membrane potential. It has no intrinsic fluorescence and emits fluorescence only upon binding to cell membranes. It is sufficiently fast to detect transient (millisecond‑scale) membrane‑potential changes in excitable cells. The magnitude of fluorescence change of Di‑4‑ANEPPDHQ is relatively consistent across various tissues, cells and membrane models, typically exhibiting a 10 % fluorescence shift per 100 mV change.

Procedure

1. Preparation of DMSO stock solution: Prepare 1‑10 mM stock solution with DMSO. Note: Aliquot unused stock solution and store at −20 °C; avoid repeated freeze‑thaw cycles.

2. Preparation of working solution: Dilute the stock solution with an appropriate buffer (e.g., serum‑free medium, PBS) to obtain a working solution of 10‑15 μM. Note: The final working concentration should be optimized according to individual experimental systems. It is recommended to explore the optimal concentration within a 10‑fold range of the suggested concentration.

Precautions

1. Briefly centrifuge the vial to spin down contents to the bottom of the tube before use.

2. Fluorescent dyes are susceptible to photobleaching. Minimize light exposure to slow down photobleaching.

3. For research use only. Not for clinical diagnosis or therapy. Not for food or pharmaceutical applications. Do not store in residential premises.

4. For personal safety and health, wear a lab coat and disposable gloves during handling.

Specifications

Specifiche e purezza

BioReagent, Biological Stain, for Fluorescence analysis, for Microscopy, ≥98%

Stabilità e conservazione

Store at -20℃ long term (12 months). Store in the dark. Desiccated.

Condizioni di conservazione di stoccaggio

Protected from light,Store at -20°C,Desiccated

Spedito in

Ice chest + Ice pads

Questo prodotto richiede spedizione a catena fredda. I servizi di terra e altri servizi economici non sono disponibili.

Grado

Biological Stain, BioReagent, per l'analisi a fluorescenza, per la microscopia

Purezza

≥98%

Nomi e identificatori

• Peso molecolare: 665.54

Proprietà chimiche e fisiche

• Solubilità: Soluble in Ethanol, DMSO and DMF.