DMEM High Glucose Medium (2×, without HEPES) - BioReagent

Cat. No.: D1525858
Disponibile su ordine
GRADE & PURITY BioReagent ? BioReagent grade — tested suitable for life-science and molecular-biology use. Use for cell culture, assays, and biochemical work needing biological compatibility.
Storage
Store at 2-8°C
Shipped In
Wet ice
★
Size
Germania (EU)
USA*
Price
Qty
250ml
D1525858-250ml
Su ordinazione · 8–12 settimane
15,53€
500ml
D1525858-500ml
Su ordinazione · 8–12 settimane
19,00€
Enter a quantity for the sizes you want to add.
🧪
Why this grade

BioReagent BioReagent for sensitive chromatographic and analytical workflows requiring minimal baseline interference.

🌡

Storage & shipping

Store at 2-8°C Ships Wet ice Check lot-specific COA for exact specifications.

📋

Quality documents

SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.

📚

Literature proof

Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.

Panoramica

DMEM (Dulbecco’s Modified Eagle Medium) is developed on the basis of MEM medium. Compared with MEM, it contains 2‑fold higher amino acids and 4‑fold higher vitamins, and is additionally supplemented with non‑essential amino acids, trace iron ions, and sodium pyruvate. DMEM was originally formulated with a glucose concentration of 1000 mg/L (low‑glucose type); later, a high‑glucose version containing 4500 mg/L glucose was also developed, and the medium is now widely used for various cell cultures. High‑glucose DMEM is commonly applied for fast‑growing, low‑adherent cells, hybridoma and myeloma cells, clonal cells, transformed cells for DNA transfection, various primary virus host cells, single‑cell cultures, and vaccine production – for example, in CHO cells for EPO expression and hepatitis B vaccine manufacturing. This product contains multiple components required for cell culture, including amino acids, vitamins, inorganic salts, etc.; however, it contains no proteins, lipids, or any growth factors. Therefore, this product must be used with serum or serum‑free supplements. This product contains L‑glutamine (1168 mg/L), sodium pyruvate (220 mg/L), phenol red (30 mg/L), D‑glucose (9000 mg/L), and NaHCO₃ (3700 mg/L), and does not contain HEPES. Precautions 1. This product has been sterilized by filtration. Aseptic techniques must be observed during handling to prevent contamination. 2. To maintain optimal performance, avoid freeze‑thaw cycles. 3. This product is intended for research or further study purposes only; it is not for diagnostic or therapeutic use.

Specifications

Specifiche e purezza
BioReagent
Condizioni di conservazione di stoccaggio
Store at 2-8°C
Spedito in
Wet ice
Questo prodotto richiede spedizione a catena fredda. I servizi di terra e altri servizi economici non sono disponibili.
Grado
BioReagent
Product Line
Prodotti biologici

Documentazione

📋 Safety Data Sheet (SDS)

Comprehensive hazard, handling, storage, and regulatory compliance document.

Download SDS →

✅ Certificate of Analysis (COA)

Lot-specific quality data. Enter your lot number to retrieve the exact COA.

Look up COA →

📊 Datasheet

Quick-reference summary of product specifications and applications.

View datasheet →

🔬 Specification Sheet

Full quality attributes and acceptance criteria for this grade.

View spec sheet →

Advanced Data

Certificati (CoA, COO, BSE/TSE e tabella di analisi)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:
Calcolatori di soluzioni
Recensioni

Recensioni dei clienti

Need help choosing the grade?

Our grade selection guide covers purity, stabilizer status, and application suitability for all variants in our catalog.

View BioReagent grade guide →

Shall we send you a message when we have discounts available?

Remind me later

Thank you! Please check your email inbox to confirm.

Oops! Notifications are disabled.