DPD Medium (without Agar, 77.6g/L) - BioReagent, Suitable for plant cell culture

Cat. No.: D1524304

GRADE & PURITY BioReagent, Suitable for plant cell culture

Synonyms

DPD Medium Base | DPD Medium for Plant Tissue Culture

Storage

Protected from light,Room temperature

Shipped In

Normal

Application

917

Panoramica

NLN Medium (Calcium Nitrate Free) excludes calcium nitrate from its formulation while maintaining complete proportions of all other nutritional components. Calcium nitrate can be supplemented as required during preparation to effectively prevent precipitation. Featuring flexible formulation adjustment, it is commonly applied for in vitro tissue culture of oil crops and Brassicaceae plants.

Product Composition：

Composition	(mg/L)
Ammonium nitrate	270
Potassium sulfate	1480
Magnesium sulfate	340
Calcium chloride	569.9
Potassium dihydrogen phosphate	80
Ferrous sulfate	27.79
Disodium EDTA	37.3
Manganese sulfate	5
Sodium molybdate	0.1
Boric acid	2
Zinc sulfate	2
Copper sulfate	0.015
Cobalt chloride	0.01
Potassium iodide	0.25
Nicotinic acid	4
Pyridoxine hydrochloride	0.7
Thiamine hydrochloride	4
Myo-inositol	100
Folic acid	0.4
Glycine	1.4
Biotin	0.04
Sucrose	20000
Mannitol	54651
2,4-Dichlorophenoxyacetic acid	1
Kinetin	0.5

Instructions for Use：

1. Dissolution: Accurately weigh 77.6 g of this product, add it into 1 L of distilled or deionized water, and stir thoroughly until completely dissolved to prepare a homogeneous medium solution.

2. pH Adjustment (Optional): If pH adjustment is required, finely tune the pH with  dilute NaOH or dilute HCl solution before sterilization. The commonly applicable pH range for plant tissue culture is 5.5～5.8, subject to specific experimental protocols. Add the solution dropwise while stirring and monitor the pH in real time to avoid over-adjustment.

3. Sterilization: Dispense the prepared medium into suitable culture vessels and perform high-pressure steam sterilization at 116 ℃ for 30 minutes.

4. Cooling and Application: After sterilization, cool the medium naturally to room temperature in a clean and sterile environment. Use it for plant tissue culture experiments with strict aseptic operation.

Precautions：

1. This product is a full inorganic medium for plant tissue culture. It is recommended to dissolve the powder at room temperature before high-pressure sterilization; heating dissolution is not advised. A small amount of precipitate after sterilization is normal and will not affect the experimental effect.

2. Store the product in a sealed, room temperature and dark environment. The shelf life is 3 years.

3. This product is for research use only. Do not use it for medical treatment, clinical diagnosis, food, cosmetics or other commercial purposes. Do not store it in residential areas.

4. The company shall not be liable for any accidents or losses caused by abnormal or improper use of this product.

Specifications

Sinonimi

DPD Medium Base | DPD Medium for Plant Tissue Culture

Specifiche e purezza

BioReagent, Suitable for plant cell culture

Stabilità e conservazione

Store at room temperature long term (36months). Store in the dark.

Condizioni di conservazione di stoccaggio

Protected from light,Room temperature

Spedito in

Normal

Grado

Adatto alla coltura di cellule vegetali, BioReagent

Proprietà chimiche e fisiche

• Sensibilità: Light-sensitive

Domande frequenti

How should this product be stored?

Store at room temperature, protected from light. The material is light-sensitive — keep it in its original opaque or amber container.

How is this product shipped?

This product ships under standard ambient conditions. No temperature-controlled packaging is required.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

What does suitable for plant cell culture mean?

suitable for plant cell culture indicates that the product has been processed and tested for cell culture and tissue engineering. Specifications may include endotoxin limits, microbial controls, sterility, low DNase/RNase/protease activity, and verified biological activity where relevant.

What does suitable for plant cell culture mean?

suitable for plant cell culture indicates that the product has been processed and tested for cell culture and tissue engineering. Specifications may include endotoxin limits, microbial controls, sterility, low DNase/RNase/protease activity, and verified biological activity where relevant.

What does BioReagent mean?

BioReagent indicates that the product has been processed and tested for biological and biochemical applications. Specifications may include endotoxin limits, microbial controls, sterility, low DNase/RNase/protease activity, and verified biological activity where relevant.