Dyrk1A-IN-3 - Moligand™, 10 mM in DMSO , CAS No.2493976-27-3

CAS: 2493976-27-3 Cat. No.: D1497306 Formula: C₁₈H₁₆N₆ Peso molecolare: 316.36

GRADE & PURITY Moligand™, 10 mM in DMSO

Storage

Store at -80°C

Shipped In

Dry ice packs + Cold packs

Panoramica

Dyrk1A-IN-3 (Compound 8b), a highly selective dual-specificity tyrosine-regulated kinase 1A ( DYRK1A ) inhibitor, maintains high levels of DYRK1A binding affinity ( IC 50 =76 nM). Dyrk1A-IN-3 can be used for the research of neurodegenerative disorders such as Alzheimer’s Disease, Huntington’s Disease, and Parkinson’s Disease.

Specifications

Specifiche e purezza

Moligand™, 10 mM in DMSO

Condizioni di conservazione di stoccaggio

Store at -80°C

Spedito in

Dry ice packs + Cold packs

Questo prodotto richiede spedizione a catena fredda. I servizi di terra e altri servizi economici non sono disponibili.

Grado

Moligand™

Tipo di azione

INHIBITOR

Nomi e identificatori

Peso molecolare

316.36

Application Protocols

No tested applications, recommended dilutions, or positive controls are provided in the product data for this item.

• Suggested protocols: Not specified for this item; refer to CoA/Spec Sheet.
• Assay types validated by the manufacturer: Not specified for this item; refer to CoA/Spec Sheet.

For internal method development, follow your laboratory’s SOPs for small-molecule additions to biochemical or cell-based assays and validate concentration ranges empirically.

Biological Roles

Item-specific biological mechanism and target binding data are not provided in this listing. The product name suggests use as a small-molecule research ligand; however, no potency, selectivity, or target specificity is claimed here.

General context for small-molecule screening ligands (literature/practice):

• Such compounds are commonly explored as chemical probes to modulate enzymatic pathways or protein–protein interactions in cellular or biochemical assays.
• They can enable target validation, pathway mapping, and tool-compound controls in phenotypic studies when used alongside orthogonal probes and genetic perturbation.
• Best practices include dose–response characterization, time-course evaluation, and orthogonal assay formats (biochemical vs cellular) to triangulate on-target effects vs assay artifacts.

Caveats:

• Without item-specific data (Kd, IC50, kinase panel selectivity, etc.), researchers should perform independent profiling to determine suitability for their system.
• Consider liabilities such as poor solubility, redox activity, photoreactivity, or aggregation, which can confound biological readouts.

Conclusion:

• Treat this item as a research-grade small molecule for exploratory studies. Obtain or generate primary data (binding, activity, ADME proxies) to define its biological role in your experimental context.

Buffer Applications

This product is a small-molecule research ligand and is not itself a buffering agent. Accordingly, classical buffer system guidance (pH ranges, recipes) does not apply.

Practical notes for use in buffered assays (general guidance):

• Prepare concentrated DMSO stocks and dilute into the chosen assay buffer while maintaining a constant final DMSO percentage across control and treated samples.
• Verify solubility and clarity upon dilution into common buffers (e.g., HEPES, PBS, Tris) at the working concentration.
• Filter sterilize aqueous working solutions only if stability permits and if required by your assay; evaluate potential adsorption to membranes.

For buffer selection and formulation specifics, follow your assay’s validated SOPs; this listing provides no item-specific buffering data.

Green Alternatives

As a fixed chemical entity, Dyrk1A-IN-3 itself does not have a direct “green alternative.” However, greener choices can be made in solvent handling and workflow design when using bioactive small molecules.

Greener solvent and process considerations (general guidance):

• Prefer DMSO over more hazardous amide solvents (e.g., DMF, NMP) for stock solutions wherever feasible; limit working concentrations to ≤0.1–0.5% v/v in aqueous assays.
• Minimize solvent volumes by preparing high-concentration stocks and using low-dead-volume dispensing.
• Use amber glass and low-temperature storage to extend shelf life, reducing waste from degraded material.

Comparison (general; not item-specific):

• DMF/NMP vs DMSO: DMF and NMP are often effective solubilizers but carry higher worker-exposure and environmental concerns; DMSO is usually the greener choice at small volumes.
• Ethanol: Renewable and lower toxicity but may provide insufficient solubility for many heteroaromatic ligands and can introduce volatility-related dosing error.

Operational tips:

• Adopt aliquoting to avoid repeated freeze–thaw and premature disposal of partially degraded stocks.
• Plan plate maps to reduce overdispense and leftover volumes.
• Employ validated, low-volume, acoustic or positive-displacement dispensing to limit solvent use and increase accuracy.

Pharmaceutical Uses

No pharmacopeial status, excipient role, or clinical/formulation use is specified for this item. It is supplied strictly for research use only.

General considerations for discovery-stage compounds (context only, not specific to this item):

• Early profiling often includes solubility screening across biorelevant media, preliminary stability (chemical and metabolic) assessments, and permeability proxies; such data are not provided here.
• Any preformulation experimentation (salt screening, solid form screening) should be undertaken only if aligned with your internal research goals and is outside the scope of this listing.

Compliance note:

• This product is not intended for human or veterinary use, diagnostics, or clinical applications. No therapeutic claims are made or implied.

Physical Properties

No compound-specific physicochemical constants are provided in the listing. Where values are required for experimental design, please consult the CoA/Spec Sheet.

• Appearance: Not specified for this item; refer to CoA/Spec Sheet.
• Molecular weight: Not specified for this item; refer to CoA/Spec Sheet.
• Melting point: Not specified for this item; refer to CoA/Spec Sheet.
• Boiling point: Not applicable/rarely reported for solid bioactives; Not specified for this item; refer to CoA/Spec Sheet.
• Density: Not specified for this item; refer to CoA/Spec Sheet.
• LogP/logD, pKa, refractive index: Not specified for this item; refer to CoA/Spec Sheet.
• Solubility: Not specified for this item; refer to CoA/Spec Sheet.

General handling guidance (literature/practice for small-molecule probes):

• Stock solutions are commonly prepared in anhydrous DMSO at 10–50 mM, then diluted into assay buffer or media; determine solubility limits empirically.
• If aqueous use is required, consider co-solvent strategies (e.g., 0.1–1% DMSO v/v) and surfactant-free conditions to minimize aggregation artifacts.
• Avoid prolonged exposure of DMSO stocks to moisture and repeated freeze–thaw cycles; aliquot upon first thaw for stability.

Important: These are general practices, not specifications for this item. Always verify with the item’s CoA and stability guidance from the SDS.

Quality and Grades

Grade/Purity for this item: Moligand™.

What Moligand™ implies (general description):

• Moligand™ denotes a research-grade small-molecule ligand suitable for screening, target validation workflows, and exploratory SAR. It emphasizes identity and fitness for use in discovery assays rather than pharmaceutical cGMP compliance.
• Typical documentation accompanying Moligand-grade items includes identity confirmation (e.g., HPLC/LC–MS) and recommended storage/shipping conditions. Exact acceptance criteria and any stabilizers, if used, should be verified in the CoA/Spec Sheet.

Item-specific notes:

• Purity percentage, stabilizers, residual solvent levels, metal or elemental impurities, and UV cutoffs are Not specified for this item; refer to CoA/Spec Sheet.
• Storage: Store at −80°C (per listing). Shipped on dry ice packs + cold packs to maintain integrity.

How to use grade information in planning experiments:

• For biochemical and cell-based assays, pre-qualify a fresh lot by verifying solubility at intended stock concentration and checking assay background signal at the working DMSO percentage.
• For orthogonal confirmation, consider analytical re-check (HPLC/LC–MS) on arrival if your workflows require trace-level impurity control.
• If working near solubility limits, filter DMSO stocks (0.2 µm PTFE) into low-binding vials to reduce particulate carryover.

Reaction and Applications

This item is supplied as a bioactive screening molecule rather than a synthetic reagent. Consequently, it is not typically used as a reagent or catalyst in preparative organic synthesis.

What is applicable:

• Discovery screening: Suitable for inclusion in small-molecule libraries for target engagement studies, phenotypic screens, and orthogonal validation experiments (general research use statement; no claims on potency or selectivity for this specific item are made here).
• Assay development: Can serve as a reference ligand in establishing assay windows, DMSO tolerance, and signal-to-background performance.

What is not typically applicable:

• Named organic reactions (e.g., Grignard, cross-coupling) or reagent-based transformations involving this compound.

Good practices for research use:

• Verify solubility at intended stock concentration in DMSO and absence of precipitation upon dilution into assay buffers.
• Control for aggregate-based interference by working at concentrations below the solubility/aggregation threshold and by including appropriate counterscreens where relevant.
• Track exposure conditions (light, temperature, pH) during assay setup to minimize degradation artifacts.

Note: No manufacturer application notes are provided in the listing. Any use should be guided by your internal assay design and validated experimentally.

Reaction Conditions

Not typically applicable. Dyrk1A-IN-3 is supplied for use as a bioactive screening molecule rather than as a reagent for chemical transformations.

If you are performing solution preparations (general guidance):

• Dissolution: Start with anhydrous DMSO; mix gently and, if necessary, warm to 25–37°C briefly to aid dissolution. Avoid prolonged heating.
• Working solutions: Dilute into assay buffer or media while maintaining a constant co-solvent percentage; mix thoroughly to avoid local supersaturation and precipitation.
• Stability checks: Monitor for turbidity or color change after dilution; assess activity and integrity after typical assay incubations.

No item-specific kinetic, catalytic, or synthetic reaction conditions are provided. Refer to the CoA/SDS for any stability cautions (light, moisture, pH) specific to this item.

Safety and Handling

Authoritative safety information is provided in the SDS accompanying the product. Product-specific GHS fields are not specified in this listing.

• Signal word: Not specified for this item; refer to SDS.
• GHS classification: Not specified for this item; refer to SDS.
• H/P statements and pictograms: Not specified for this item; refer to SDS.

General laboratory precautions for small-molecule research chemicals:

• PPE: Use a lab coat, nitrile gloves, and splash-resistant safety glasses as a minimum. Handle powders and DMSO solutions in a chemical fume hood.
• Incompatibilities: Avoid strong oxidizers and strong acids/bases unless compatibility is established. Keep away from ignition sources when using organic solvents.
• First aid overview: In case of skin/eye contact, rinse with water for at least 15 minutes; remove contaminated clothing. If inhaled, move to fresh air. If ingested, rinse mouth; do not induce vomiting. Seek medical attention for all exposures. See SDS for details.
• Spill response: Absorb with inert material (e.g., vermiculite), collect for disposal as hazardous waste. Decontaminate surfaces with appropriate solvent/detergent as per SDS.
• Waste: Dispose of solutions and solids as hazardous chemical waste following institutional and local regulations.

Storage risk controls:

• Store at −80°C as specified; protect from light and moisture. Maintain material in tightly sealed containers. Minimize freeze–thaw cycles by aliquoting upon receipt.

Solvent Selection

This product is a bioactive small molecule typically handled as concentrated DMSO stocks and diluted into assay-compatible media.

General solvent guidance for small-molecule ligands (literature/practice):

• Primary solvent: DMSO (high polarity, aprotic; dielectric constant ~47 at 20°C, literature). Widely used for 10–50 mM stocks.
• Alternative co-solvents (as needed): DMF, NMP, or ethanol for initial dissolution; subsequently dilute into buffer/media maintaining ≤0.1–0.5% v/v organic content to protect biological systems.
• Aqueous use: Employ co-solvent strategies and pre-warm solutions to improve dissolution; avoid surfactants unless justified by the assay.

Small comparison (general, not item-specific):

• DMSO vs DMF: DMSO is preferred for bioassays due to lower volatility and established biocompatibility at low percentages; DMF/NMP may enhance solubility but can be more cytotoxic.
• Ethanol: Useful for some hydrophobic scaffolds; volatility and lower water miscibility can complicate accurate dosing.

Practical tips:

• Prepare a small test stock at target concentration to confirm clarity and stability over 24–48 h at −20°C and room temperature.
• Use amber vials to limit photodegradation if the compound contains chromophores (structure not provided; proceed cautiously).
• Always confirm solvent compatibility with your assay components and target system.

Storage and Reconstitution

Storage and shipping (item-specific):

• Storage condition: Store at −80°C.
• Shipped in: Dry ice packs + Cold packs to preserve material integrity.

Reconstitution and handling (general best practices for bioactive small molecules; not specifications):

• Upon receipt, allow the container to equilibrate to room temperature in a desiccator before opening to avoid moisture condensation.
• Prepare concentrated stocks in anhydrous DMSO (common working range 10–50 mM, limited by solubility). Verify clarity and absence of particulates.
• Aliquot stocks into low-binding, amber vials to minimize freeze–thaw cycles and light exposure.
• Short-term storage of DMSO stocks: −20°C to −30°C; long-term storage: −80°C as per item guidance. Avoid repeated thawing; thaw only the aliquot needed for immediate use.
• For aqueous working solutions, prepare fresh immediately before use. Discard if turbidity or precipitation occurs.

Additional notes:

• Appearance, exact solubility, and stability limits are Not specified for this item; refer to CoA/Spec Sheet and SDS.
• Always label aliquots with concentration, solvent, date, and lot number to maintain full traceability.

Structure and Identity

Brief overview: Dyrk1A-IN-3 is supplied as a Moligand-grade small molecule for research screening libraries. Item-specific structural identifiers are not provided in the current listing.

• Product Name: Dyrk1A-IN-3 (research chemical; small-molecule library member)
• CAS: 2493976-27-3
• Molecular formula: Not specified for this item; refer to CoA/Spec Sheet.
• Molecular weight: Not specified for this item; refer to CoA/Spec Sheet.
• SMILES: Not specified for this item; refer to CoA/Spec Sheet.
• InChIKey: Not specified for this item; refer to CoA/Spec Sheet.

Structural features (general, literature-style context):

• Functional class: Bioactive small molecule (screening ligand); exact scaffold and substituents not disclosed here.
• Stereochemistry: Not specified for this item; refer to CoA/Spec Sheet.
• 2D structural description: Not available in the product data; typically, compounds in this category feature heteroaromatic cores and H-bonding motifs, but the exact architecture should be confirmed from the CoA/SDS.

Notes:

• For definitive identity confirmation (e.g., HRMS, NMR, HPLC trace), consult the item’s CoA/Spec Sheet/SDS provided with the shipment or available upon request.

Synthetic Utility

This item is a finished bioactive small molecule intended for screening and is not typically employed as a reagent, catalyst, or building block in synthetic chemistry.

Implications:

• Functional-group reactivity, protecting-group strategies, or incorporation into multistep synthetic routes are not generally relevant to its intended use.
• If derivatization or analog synthesis is pursued for SAR, obtain the full structural information from the CoA/Spec Sheet or literature and plan route scouting accordingly.

General notes (for researchers considering analog work):

• If the scaffold is heteroaromatic (common for kinase-oriented ligands), late-stage diversification via cross-coupling or nucleophilic substitutions may be possible; however, no structure is provided here to guide such tactics.
• Analytical controls (HPLC/LC–MS, NMR) and purity criteria should be established in-house when preparing derivatives for research comparisons.

Conclusion: Treat Dyrk1A-IN-3 as a screening ligand; synthetic utility is limited unless you have structural details and a programmatic need for analog generation.

Target Specificity

Target, epitope, and specificity information are not provided in the product data for this item.

• Target name/class: Not specified for this item; refer to CoA/Spec Sheet.
• Binding parameters (Kd, IC50, EC50), mode of action, and selectivity panels: Not specified for this item; refer to CoA/Spec Sheet.
• Species cross-reactivity or off-target profiling: Not specified for this item; refer to CoA/Spec Sheet.

Researchers should generate or request primary data to establish specificity within their experimental systems.

Domande frequenti

What is Moligand??

Moligand? is one of Aladdin Scientific's premium product lines, formulated for high-purity applications across multiple workflows. Compared to standard grades in the same workflow, Moligand? products typically offer tighter specifications, more rigorous QC, and stronger lot-to-lot consistency.

How should this product be stored?

Store at ?80 °C. Ultra-low temperature storage is required to maintain the specified shelf life.

How is this product shipped?

This product ships frozen with dry ice and cold packs. Unpack on arrival and transfer it to the storage condition stated above; handle dry ice with insulated gloves in a ventilated area.

What are the CAS number, molecular formula and molecular weight?

The CAS Number is 2493976-27-3, the molecular formula is C18H16N6, and the molecular weight is 316.36 g/mol.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.