E. coli Genomic DNA Extraction Kit (CTAB Precipitation Method)

Cat. No.: E1527100

GRADE & PURITY BioReagent

Panoramica

Escherichia coli (E. coli) is a Gram-negative, short rod-shaped bacterium, approximately 0.5 µm × (1–3 µm) in size. It is peritrichously flagellated, motile, non-spore-forming, and capable of fermenting various sugars to produce acid and gas. It is a normal commensal inhabitant of the intestinal tract of humans and animals. After birth, E. coli colonizes the infant gut through feeding and remains throughout life, accounting for approximately one-third of the fecal dry weight. DNA obtained from E. coli is of relatively high yield but moderate purity, which is generally sufficient for most molecular biology experiments. CTAB (cetyltrimethylammonium bromide) is a cationic detergent. In solutions of high ionic strength, CTAB forms complexes with proteins and polysaccharides (with the exception of most acidic polysaccharides), but does not bind to nucleic acids. By adjusting the salt concentration, nucleic acids can be released from CTAB complexes and precipitated, enabling DNA purification from polysaccharide-rich organisms (such as plants and certain Gram-negative bacteria). This product is a convenient CTAB-based genomic DNA extraction kit for E. coli. The principle is as follows: proteins are digested by proteinase K; polysaccharides are precipitated by CTAB; proteins are denatured and separated from DNA by heating; and finally, genomic DNA is obtained by ethanol precipitation. The resulting DNA can be used in downstream applications such as restriction enzyme digestion and PCR. This kit is intended for research use only and is not suitable for clinical diagnosis or other applications.

Specifications

Sinonimi

Escherichia coli genomic DNA extraction kit

Specifiche e purezza

BioReagent

Grado

BioReagent

Applicazione

DNA Extraction

Sensibilità

Light Sensitive

Stoccaggio e spedizione

Condizioni di conservazione di stoccaggio

Store at 2-8°C,Protected from light,Room temperature,Store at -20°C

Spedito in

Normal,Wet ice,Ice chest + Ice pads

Stabilità e conservazione

Each component has a shelf life of 12 months under corresponding storage conditions.

Contents & Storage

Storage Unit No.	Storage Unit Name	Component No.	Component Name	Appearance	50T	Storage
E1527100NT	E. coli Genomic DNA Extraction Kit (CTAB Precipitation Method)(RT Components)	E1527100A	E. coli Lysis Buffer	Liquid	1.5ml	Room temperature
		E1527100B	CTAB Precipitation Solution	Liquid	10ml	Room temperature
		E1527100C	E. coli Wash Buffer	Liquid	100ml	Room temperature
		E1527100D	TE Buffer	Liquid	50ml	Room temperature
E1527100WT	E. coli Genomic DNA Extraction Kit (CTAB Precipitation Method)(2-8℃ Components)	E1527100E	Protein Removal Solution	Liquid	50ml	2-8℃.Store in the dark.
E1527100DT	E. coli Genomic DNA Extraction Kit (CTAB Precipitation Method)(-20℃ Components)	E1527100F	Proteinase K Solution	Liquid	0.5ml	-20℃

Note: Each component is individually packaged according to its storage temperature (Room temperature/2‑8 °C / -20 °C) upon shipment, with differentiated temperature‑controlled transportation to guarantee product performance. Orders containing materials requiring multiple temperature zones may be delivered in separate batches; please refer to the actual received goods. Upon receipt, store components separately at the temperatures specified in the component list. The shelf‑life is 12 months from the date of manufacture.