Edrecolomab ELISA Kit

Cat. No.: EJ1515744

GRADE & PURITY BioReagent

Panoramica

This kit employs the indirect enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with Human CD326 antigen. The sample or standard is first added to the wells, followed by an HRP (horseradish peroxidase)-labeled secondary antibody. The procedure involves incubation and wash steps between each addition. TMB substrate is then added for color development. Under the catalysis of the peroxidase (HRP), TMB is converted into a blue product, which subsequently turns into a final stable yellow color upon the addition of an acid stop solution. The intensity of the yellow color is directly proportional to the concentration of Edrecolomab in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader, and the concentration of the target analyte in the sample is calculated based on the standard curve.

Specifications

Specifiche e purezza

BioReagent

Grado

BioReagent

Applicazione

ELISA

Tipo di campione

Lisato cellulare, omogenati tissutali, Plasma, Siero, supernatanti di colture cellulari

Campo di rilevamento

0.78ng/mL~50ng/mL

Specie rilevata Oggetto

Human

Specificità

Detecting Human Edrecolomab in samples, with no significant cross-reactivity to other homologs.

Detection instrument

Microplate reader

Detection wavelength

450nm

Repeatability

The intra- and inter-assay coefficients of variation (CV) are both <10%.

Detection duration

3.5 hours, not including equilibration and sample preparation time.

Stoccaggio e spedizione

Condizioni di conservazione di stoccaggio

Store at 2-8°C

Spedito in

Wet ice

Stabilità e conservazione

Store at 2-8℃ for 6 months.