GRADE & PURITYBioReagent?BioReagent grade — tested suitable for life-science and molecular-biology use. Use for cell culture, assays, and biochemical work needing biological compatibility.for Protein analysis?Protein-analysis grade — low-interference reagents for protein quantitation/characterization. Use in protein assays where purity affects accuracy.for ELISA?ELISA grade — low-background reagents validated for enzyme immunoassays. Use to build sensitive, reproducible ELISA assays.0.5M
BioReagent,for protein analysis,for ELISA,0.5M BioReagent,for ELISA,for Protein analysis for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Room temperature Ships Normal Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
STOP Solution is a proprietary solution used to terminate the peroxidase/TMB reaction for ELISA applications. The TMB substrate reacts with immobilized horseradish peroxidase (HRP) conjugated secondary antibodies to produce a blue solution. Color intensity is an indication of analyte level. After attaining the desired intensity, the reaction is terminated by addition of STOP Solution. Upon addition of STOP Solution the color turns from blue to yellow.
Instructions for Use：
Following TMB incubation, add an amount of stop solution equal to the amount of TMB substrate added to each well of the ELISA plate (typically 100 μL per well). Use at full strength.
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