Endo F1

Cat. No.: E489799

GRADE & PURITY Specific Activity ＞16 U/mg;Activity ＞17 U/ml

Bioactivity

>17 U/ml

Overview

Product Description

Endo F1, Endoglycosidase F1, endo-beta-N-acetylglucosaminidase F

Endo F1 cleaves Asparagine-linked high mannose and some hybrid oligosaccharides. Core fucosylation reduces the activity by 50 fold. Endoglycosidase F1 will hydrolyze sulfate containing high-mannose chains. It cleaves between the two N-acetylglucosamine residues in the diacetylchitobiose core of the oligosaccharide, generating a truncated sugar molecule with one N-acetylglucosamine residue remaining on the asparagine. In contrast, PNGase F removes the oligosaccharide intact.

Endo F1 Specifictity Cleaves all asparagine-linked high mannose and some hybrid oligosaccharides

Source recombinant Elizabethkingia miricola (was Chryseobacterium meningosepticum) in E. Coli

EC 3.2.1.96

Specific Activity >16 U/mg

Activity >17 U/ml

Molecular weight 32,000 daltons

Specific Activity

Defined as the amount of enzyme required to catalyze the release of N-linked oligosaccharides from 1 micromole of denatured Ribonuclease B (RNase B) in 1 minute at 37˚C, pH 5.5. Cleavage is monitored by SDS-PAGE (cleaved RNase B migrates faster).

Contents

60 µl aliquot of enzyme (1 U) in 20 mM Tris-HCl, pH 7.5

Included with 20 µL and 60 µL pack sizes:

5x Reaction Buffer – 250 mM sodium phosphate, pH 5.5

Suggested usage

1. Add up to 200 µg of glycoprotein to an Eppendorf tube. Adjust to 38 µl final volume with de-ionized water.

2. Add 10 µl 5x Reaction Buffer 5.5

3. Add 2.0 µl of Endo F1. Incubate 1 hour at 37ˆC.

Storage

Store enzyme at 4˚C.

Stability Stable at least 12 months when stored properly. Several days exposure to ambient tempertures will not reduce activity.

Purity Endoglycosidase F1 is tested for contaminating protease as follows; 10 μg of denatured BSA is incubated for 24 hours at 37oC with 2 μL of enzyme. SDS-PAGE analysis of the treated BSA shows no evidence of degradation.

The production host strain has been extensively tested and does not produce any detectable glycosidases.

Specifications

Product Name

Endo F1

Synonyms

Endo F1 | Endoglycosidase F1 | endo-beta-N-acetylglucosaminidase F1

Specifications & Purity

Specific Activity ＞16 U/mg;Activity ＞17 U/ml

Bioactivity

>17 U/ml

Enzyme Commission Number

3.2.1.96

Molecule Type

Small molecule

Storage and Shipping

Concentration

Specific Activity ＞16 U/mg;Activity ＞17 U/ml

Storage

Store at 2-8°C

Shipped In

Wet ice